Abstract
We have shown previously that activin A increases the number of immunoreactive follicle-stimulating hormone (FSH) cells. To further investigate the action of activin A, we examined its effects on anterior pituitary cells fractionated by centrifugal elutriation. Before activin A treatment, FSH cells were widely distributed among various fractions; a higher proportion of FSH cells was found in larger cell fractions (fractions 5-9), and a lower proportion in smaller cell fractions (fractions 2-4). After culture of the cells in each fraction with activin A (10 ng/ml) for 72 h, the number of FSH cells in fraction 4 only was significantly (P<0.05) higher by 225% than that in cells cultured without activin A. The amount of FSH secreted into the medium was minimal or undetectable in fractions 1-4. However, FSH secretion tended to be, or was significantly (P
| Original language | English |
|---|---|
| Pages (from-to) | 167-173 |
| Number of pages | 7 |
| Journal | Molecular and Cellular Endocrinology |
| Volume | 77 |
| Issue number | 1-3 |
| DOIs | |
| Publication status | Published - 1991 |
| Externally published | Yes |
Keywords
- Activin A
- Anterior pituitary
- Centrifugal elutriation
- Follicle-stimulating hormone
ASJC Scopus subject areas
- Endocrinology
- Endocrinology, Diabetes and Metabolism
