Effects of activin A on anterior pituitary cells fractionated by centrifugal elutriation

Tetsuro Katayama, Kunio Shiota, Michio Takahashi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have shown previously that activin A increases the number of immunoreactive follicle-stimulating hormone (FSH) cells. To further investigate the action of activin A, we examined its effects on anterior pituitary cells fractionated by centrifugal elutriation. Before activin A treatment, FSH cells were widely distributed among various fractions; a higher proportion of FSH cells was found in larger cell fractions (fractions 5-9), and a lower proportion in smaller cell fractions (fractions 2-4). After culture of the cells in each fraction with activin A (10 ng/ml) for 72 h, the number of FSH cells in fraction 4 only was significantly (P<0.05) higher by 225% than that in cells cultured without activin A. The amount of FSH secreted into the medium was minimal or undetectable in fractions 1-4. However, FSH secretion tended to be, or was significantly (P

Original languageEnglish
Pages (from-to)167-173
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume77
Issue number1-3
DOIs
Publication statusPublished - 1991
Externally publishedYes

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Follicle Stimulating Hormone
activin A
Cultured Cells
Cell Culture Techniques

Keywords

  • Activin A
  • Anterior pituitary
  • Centrifugal elutriation
  • Follicle-stimulating hormone

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Effects of activin A on anterior pituitary cells fractionated by centrifugal elutriation. / Katayama, Tetsuro; Shiota, Kunio; Takahashi, Michio.

In: Molecular and Cellular Endocrinology, Vol. 77, No. 1-3, 1991, p. 167-173.

Research output: Contribution to journalArticle

Katayama, Tetsuro ; Shiota, Kunio ; Takahashi, Michio. / Effects of activin A on anterior pituitary cells fractionated by centrifugal elutriation. In: Molecular and Cellular Endocrinology. 1991 ; Vol. 77, No. 1-3. pp. 167-173.
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