Effects of astaxanthin on lipopolysaccharide-induced inflammation in vitro and in vivo

Kazuhiro Ohgami, Kenji Shiratori, Satoshi Kotake, Tomomi Nishida, Nobuhisa Mizuki, Kazunaga Yazawa, Shigeaki Ohno

Research output: Contribution to journalArticle

190 Citations (Scopus)

Abstract

PURPOSE. Astaxanthin (AST) is a carotenoid that is found in marine animals and vegetables. Several previous studies have demonstrated that AST exhibits a wide variety of biological activities including antioxidant, antitumor, and anti-Helicobacter pylori effects. In this study, attention was focused on the antioxidant effect of AST. The object of the present study was to investigate the efficacy of AST in endotoxin-induced uveitis (EIU) in rats. In addition, the effect of AST on endotoxininduced nitric oxide (NO), prostaglandin E2 (PGE2), and tumor necrosis factor (TNF)-α production in a mouse macrophage cell line (RAW 264.7) was studied in vitro. METHODS. EIU was induced in male Lewis rats by a footpad injection of lipopolysaccharide (LPS). AST or prednisolone was administered intravenously at 30 minutes before, at the same time as, or at 30 minutes after LPS treatment. The number of infiltrating cells and protein concentration in the aqueous humor collected at 24 hours after LPS treatment was determined. RAW 264.7 cells were pretreated with various concentrations of AST for 24 hours and subsequently stimulated with 10 μg/mL of LPS for 24 hours. The levels of PGE2, TNF-α, and NO production were determined in vivo and in vitro. RESULTS. AST suppressed the development of EIU in a dosedependent fashion. The anti-inflammatory effect of 100 mg/kg AST was as strong as that of 10 mg/kg prednisolone. AST also decreased production of NO, activity of inducible nitric oxide synthase (NOS), and production of PGE2 and TNF-α in RAW264.7 cells in vitro in a dose-dependent manner. CONCLUSIONS. This study suggests that AST has a dose-dependent ocular anti-inflammatory effect, by the suppression of NO, PGE2, and TNF-α production, through directly blocking NOS enzyme activity.

Original languageEnglish
Pages (from-to)2694-2701
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume44
Issue number6
DOIs
Publication statusPublished - 2003 Jun 1
Externally publishedYes

Fingerprint

Lipopolysaccharides
Inflammation
Dinoprostone
Uveitis
Nitric Oxide
Tumor Necrosis Factor-alpha
Endotoxins
Prednisolone
In Vitro Techniques
astaxanthine
Anti-Inflammatory Agents
Antioxidants
Aqueous Humor
Nitric Oxide Synthase Type II
Carotenoids
Helicobacter pylori
Nitric Oxide Synthase
Vegetables
Cell Count
Macrophages

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Ohgami, K., Shiratori, K., Kotake, S., Nishida, T., Mizuki, N., Yazawa, K., & Ohno, S. (2003). Effects of astaxanthin on lipopolysaccharide-induced inflammation in vitro and in vivo. Investigative Ophthalmology and Visual Science, 44(6), 2694-2701. https://doi.org/10.1167/iovs.02-0822

Effects of astaxanthin on lipopolysaccharide-induced inflammation in vitro and in vivo. / Ohgami, Kazuhiro; Shiratori, Kenji; Kotake, Satoshi; Nishida, Tomomi; Mizuki, Nobuhisa; Yazawa, Kazunaga; Ohno, Shigeaki.

In: Investigative Ophthalmology and Visual Science, Vol. 44, No. 6, 01.06.2003, p. 2694-2701.

Research output: Contribution to journalArticle

Ohgami, K, Shiratori, K, Kotake, S, Nishida, T, Mizuki, N, Yazawa, K & Ohno, S 2003, 'Effects of astaxanthin on lipopolysaccharide-induced inflammation in vitro and in vivo', Investigative Ophthalmology and Visual Science, vol. 44, no. 6, pp. 2694-2701. https://doi.org/10.1167/iovs.02-0822
Ohgami, Kazuhiro ; Shiratori, Kenji ; Kotake, Satoshi ; Nishida, Tomomi ; Mizuki, Nobuhisa ; Yazawa, Kazunaga ; Ohno, Shigeaki. / Effects of astaxanthin on lipopolysaccharide-induced inflammation in vitro and in vivo. In: Investigative Ophthalmology and Visual Science. 2003 ; Vol. 44, No. 6. pp. 2694-2701.
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AU - Mizuki, Nobuhisa

AU - Yazawa, Kazunaga

AU - Ohno, Shigeaki

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