Effects of caffeine on circadian phase, amplitude and period evaluated in cells in vitro and peripheral organs in vivo in PER2::LUCIFERASE mice

Seira Narishige, Mari Kuwahara, Ayako Shinozaki, Satoshi Okada, Yuko Ikeda, Mayo Kamagata, Yu Tahara, Shigenobu Shibata

Research output: Contribution to journalArticle

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Abstract

Background and Purpose Caffeine is one of the most commonly used psychoactive substances. Circadian rhythms consist of the main suprachiasmatic nucleus (SCN) clocks and peripheral clocks. Although caffeine lengthens circadian rhythms and modifies phase changes in SCN-operated rhythms, the effects on caffeine on the phase, period and amplitude of peripheral organ clocks are not known. In addition, the role of cAMP/Ca2+ signalling in effects of caffeine on rhythm has not been fully elucidated. Experimental Approach We examined whether chronic or transient application of caffeine affects circadian period/amplitude and phase by evaluating bioluminescence rhythm in PER2::LUCIFERASE knock-in mice. Circadian rhythms were monitored in vitro using fibroblasts and ex vivo and in vivo for monitoring of peripheral clocks. Key Results Chronic application of caffeine (0.1-10 mM) increased period and amplitude in vitro. Transient application of caffeine (10 mM) near the bottom of the decreasing phase of bioluminescence rhythm caused phase advance in vitro. Caffeine (0.1%) intake caused a phase delay under light-dark or constant dark conditions, suggesting a period-lengthening effect in vivo. Caffeine (20 mg·kg-1) at daytime or at late night-time caused phase advance or delay in bioluminescence rhythm in the liver and kidney respectively. The complicated roles of cAMP/Ca2+ signalling may be involved in the caffeine-induced increase of period and amplitude in vitro. Conclusions and Implications Caffeine affects circadian rhythm in mice by lengthening the period and causing a phase shift of peripheral clocks. These results suggest that caffeine intake with food/drink may help with food-induced resetting of peripheral circadian clocks.

Original languageEnglish
Pages (from-to)5858-5869
Number of pages12
JournalBritish Journal of Pharmacology
Volume171
Issue number24
DOIs
Publication statusPublished - 2014

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Caffeine
Circadian Rhythm
Suprachiasmatic Nucleus
In Vitro Techniques
Circadian Clocks
Fibroblasts
Eating
Kidney
Light
Food

ASJC Scopus subject areas

  • Pharmacology

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Effects of caffeine on circadian phase, amplitude and period evaluated in cells in vitro and peripheral organs in vivo in PER2::LUCIFERASE mice. / Narishige, Seira; Kuwahara, Mari; Shinozaki, Ayako; Okada, Satoshi; Ikeda, Yuko; Kamagata, Mayo; Tahara, Yu; Shibata, Shigenobu.

In: British Journal of Pharmacology, Vol. 171, No. 24, 2014, p. 5858-5869.

Research output: Contribution to journalArticle

Narishige, Seira ; Kuwahara, Mari ; Shinozaki, Ayako ; Okada, Satoshi ; Ikeda, Yuko ; Kamagata, Mayo ; Tahara, Yu ; Shibata, Shigenobu. / Effects of caffeine on circadian phase, amplitude and period evaluated in cells in vitro and peripheral organs in vivo in PER2::LUCIFERASE mice. In: British Journal of Pharmacology. 2014 ; Vol. 171, No. 24. pp. 5858-5869.
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AU - Okada, Satoshi

AU - Ikeda, Yuko

AU - Kamagata, Mayo

AU - Tahara, Yu

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