Effects of caloric restriction on gene expression in the arcuate nucleus

Isao Shimokawa*, Tamaki Fukuyama, Kurumi Yanagihara-Outa, Masato Tomita, Toshimitsu Komatsu, Yoshikazu Higami, Tomoshi Tuchiya, Takuya Chiba, Yoshiharu Yamaza

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


Neuroendocrine alterations that repress energy-costly physiologic processes such as reproduction and growth and induce stress responses, might underlie the antiaging effect of caloric restriction (CR). Neurons in the arcuate nucleus of the hypothalamus (ARH) might have a pivotal role in these neuroendocrine alterations. We investigated the effects of CR on gene expression of neuropeptide Y (NPY), proopiomelanocortin (POMC), growth hormone-releasing hormone (GHRH), somatostatin (SRIH), and cyclophilin (CP) in the ARH in male F344 rats at 6 months of age. Rats were fed ad libitum or a 30% caloric restricted diet with a modified alternate-days feeding regimen from 6 weeks of age. Reverse transcription-polymerase chain reaction (RT-PCR) methods were used to quantify mRNA levels over multiple time points during the 12-h/12-h dark/light cycle over a 2-days feeding cycle. The present study demonstrated that CR increased NPY-mRNA levels, but decreased POMC, GHRH, and CP mRNA levels differentially over the feeding cycle. The SRIH level was not significantly affected by CR. The present results support the neuroendocrine hypothesis of CR.

Original languageEnglish
Pages (from-to)117-123
Number of pages7
JournalNeurobiology of Aging
Issue number1
Publication statusPublished - 2003 Jan
Externally publishedYes


  • Arcuate nucleus
  • Caloric restriction
  • Circadian rhythm
  • Gene expression
  • Growth hormone-releasing hormone
  • Hypothalamus
  • Neuroendocrine
  • Neuropeptide Y
  • Proopiomelanocortin
  • Somatostatin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Ageing
  • Clinical Neurology
  • Developmental Biology
  • Geriatrics and Gerontology


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