Effects of caloric restriction on gene expression in the arcuate nucleus

Isao Shimokawa, Tamaki Fukuyama, Kurumi Yanagihara-Outa, Masato Tomita, Toshimitsu Komatsu, Yoshikazu Higami, Tomoshi Tuchiya, Takuya Chiba, Yoshiharu Yamaza

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Neuroendocrine alterations that repress energy-costly physiologic processes such as reproduction and growth and induce stress responses, might underlie the antiaging effect of caloric restriction (CR). Neurons in the arcuate nucleus of the hypothalamus (ARH) might have a pivotal role in these neuroendocrine alterations. We investigated the effects of CR on gene expression of neuropeptide Y (NPY), proopiomelanocortin (POMC), growth hormone-releasing hormone (GHRH), somatostatin (SRIH), and cyclophilin (CP) in the ARH in male F344 rats at 6 months of age. Rats were fed ad libitum or a 30% caloric restricted diet with a modified alternate-days feeding regimen from 6 weeks of age. Reverse transcription-polymerase chain reaction (RT-PCR) methods were used to quantify mRNA levels over multiple time points during the 12-h/12-h dark/light cycle over a 2-days feeding cycle. The present study demonstrated that CR increased NPY-mRNA levels, but decreased POMC, GHRH, and CP mRNA levels differentially over the feeding cycle. The SRIH level was not significantly affected by CR. The present results support the neuroendocrine hypothesis of CR.

Original languageEnglish
Pages (from-to)117-123
Number of pages7
JournalNeurobiology of Aging
Volume24
Issue number1
DOIs
Publication statusPublished - 2003 Jan
Externally publishedYes

Fingerprint

Caloric Restriction
Arcuate Nucleus of Hypothalamus
Gene Expression
Cyclophilins
Growth Hormone-Releasing Hormone
Pro-Opiomelanocortin
Neuropeptide Y
Messenger RNA
Inbred F344 Rats
Photoperiod
Somatostatin
Reverse Transcription
Reproduction
Diet
Neurons
Polymerase Chain Reaction
Growth

Keywords

  • Arcuate nucleus
  • Caloric restriction
  • Circadian rhythm
  • Gene expression
  • Growth hormone-releasing hormone
  • Hypothalamus
  • Neuroendocrine
  • Neuropeptide Y
  • Proopiomelanocortin
  • Somatostatin

ASJC Scopus subject areas

  • Clinical Neurology
  • Biological Psychiatry
  • Developmental Neuroscience
  • Neurology
  • Psychology(all)

Cite this

Shimokawa, I., Fukuyama, T., Yanagihara-Outa, K., Tomita, M., Komatsu, T., Higami, Y., ... Yamaza, Y. (2003). Effects of caloric restriction on gene expression in the arcuate nucleus. Neurobiology of Aging, 24(1), 117-123. https://doi.org/10.1016/S0197-4580(02)00046-5

Effects of caloric restriction on gene expression in the arcuate nucleus. / Shimokawa, Isao; Fukuyama, Tamaki; Yanagihara-Outa, Kurumi; Tomita, Masato; Komatsu, Toshimitsu; Higami, Yoshikazu; Tuchiya, Tomoshi; Chiba, Takuya; Yamaza, Yoshiharu.

In: Neurobiology of Aging, Vol. 24, No. 1, 01.2003, p. 117-123.

Research output: Contribution to journalArticle

Shimokawa, I, Fukuyama, T, Yanagihara-Outa, K, Tomita, M, Komatsu, T, Higami, Y, Tuchiya, T, Chiba, T & Yamaza, Y 2003, 'Effects of caloric restriction on gene expression in the arcuate nucleus', Neurobiology of Aging, vol. 24, no. 1, pp. 117-123. https://doi.org/10.1016/S0197-4580(02)00046-5
Shimokawa I, Fukuyama T, Yanagihara-Outa K, Tomita M, Komatsu T, Higami Y et al. Effects of caloric restriction on gene expression in the arcuate nucleus. Neurobiology of Aging. 2003 Jan;24(1):117-123. https://doi.org/10.1016/S0197-4580(02)00046-5
Shimokawa, Isao ; Fukuyama, Tamaki ; Yanagihara-Outa, Kurumi ; Tomita, Masato ; Komatsu, Toshimitsu ; Higami, Yoshikazu ; Tuchiya, Tomoshi ; Chiba, Takuya ; Yamaza, Yoshiharu. / Effects of caloric restriction on gene expression in the arcuate nucleus. In: Neurobiology of Aging. 2003 ; Vol. 24, No. 1. pp. 117-123.
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