Abstract
Carbocisteine is a mucoregulatory drug regulating fucose and sialic acid contents in mucus glycoprotein. To investigate the mechanism of carbocisteine action, we evaluated the effects of carbocisteine on the activity of fucosidase, sialidase, fucosyltransferase and sialyltransferase, and on the expression of Muc5ac mRNA in the airway epithelium of SO2-exposed rats. Wistar rats were repeatedly exposed to a 300-ppm SO2 gas for 44 days. Carbocisteine (125 and 250 mg/kg ×2/day) was administered for 25 days after 20 days of SO2 gas exposure. These enzyme activities were measured by fluorogenic substrate or glycoproteinic exogenous acceptor method. The expression levels of Muc5ac mRNA and protein were determined with real-time reverse transcriptase-polymerase chain reaction (real-time RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Carbocisteine (250 mg/kg ×2/day) inhibited all the changes in these enzyme activities and the expressions of Muc5ac mRNA and protein in the lung after repeated SO 2 exposure. These findings suggest that carbocisteine may normalize fucose and sialic acid contents in mucin glycoprotein through regulation of these enzyme activities, and inhibition of both Muc5ac mRNA and protein expressions in SO2-exposed rats.
| Original language | English |
|---|---|
| Pages (from-to) | 7-15 |
| Number of pages | 9 |
| Journal | European Journal of Pharmacology |
| Volume | 487 |
| Issue number | 1-3 |
| DOIs | |
| Publication status | Published - 2004 Mar 8 |
| Externally published | Yes |
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Keywords
- Carbocisteine
- Fucosidase
- Fucosyltransferase
- Muc5ac
- Sialidase
- Sialyltransferase
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience
- Pharmacology
Cite this
Effects of carbocisteine on altered activities of glycosidase and glycosyltransferase and expression of Muc5ac in SO2-exposed rats. / Ishibashi, Yuji; Kobayashi, Fumiyoshi; Idesawa, Akira; Taniguchi, Akiyoshi; Matsuzawa, Shigeki.
In: European Journal of Pharmacology, Vol. 487, No. 1-3, 08.03.2004, p. 7-15.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of carbocisteine on altered activities of glycosidase and glycosyltransferase and expression of Muc5ac in SO2-exposed rats
AU - Ishibashi, Yuji
AU - Kobayashi, Fumiyoshi
AU - Idesawa, Akira
AU - Taniguchi, Akiyoshi
AU - Matsuzawa, Shigeki
PY - 2004/3/8
Y1 - 2004/3/8
N2 - Carbocisteine is a mucoregulatory drug regulating fucose and sialic acid contents in mucus glycoprotein. To investigate the mechanism of carbocisteine action, we evaluated the effects of carbocisteine on the activity of fucosidase, sialidase, fucosyltransferase and sialyltransferase, and on the expression of Muc5ac mRNA in the airway epithelium of SO2-exposed rats. Wistar rats were repeatedly exposed to a 300-ppm SO2 gas for 44 days. Carbocisteine (125 and 250 mg/kg ×2/day) was administered for 25 days after 20 days of SO2 gas exposure. These enzyme activities were measured by fluorogenic substrate or glycoproteinic exogenous acceptor method. The expression levels of Muc5ac mRNA and protein were determined with real-time reverse transcriptase-polymerase chain reaction (real-time RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Carbocisteine (250 mg/kg ×2/day) inhibited all the changes in these enzyme activities and the expressions of Muc5ac mRNA and protein in the lung after repeated SO 2 exposure. These findings suggest that carbocisteine may normalize fucose and sialic acid contents in mucin glycoprotein through regulation of these enzyme activities, and inhibition of both Muc5ac mRNA and protein expressions in SO2-exposed rats.
AB - Carbocisteine is a mucoregulatory drug regulating fucose and sialic acid contents in mucus glycoprotein. To investigate the mechanism of carbocisteine action, we evaluated the effects of carbocisteine on the activity of fucosidase, sialidase, fucosyltransferase and sialyltransferase, and on the expression of Muc5ac mRNA in the airway epithelium of SO2-exposed rats. Wistar rats were repeatedly exposed to a 300-ppm SO2 gas for 44 days. Carbocisteine (125 and 250 mg/kg ×2/day) was administered for 25 days after 20 days of SO2 gas exposure. These enzyme activities were measured by fluorogenic substrate or glycoproteinic exogenous acceptor method. The expression levels of Muc5ac mRNA and protein were determined with real-time reverse transcriptase-polymerase chain reaction (real-time RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Carbocisteine (250 mg/kg ×2/day) inhibited all the changes in these enzyme activities and the expressions of Muc5ac mRNA and protein in the lung after repeated SO 2 exposure. These findings suggest that carbocisteine may normalize fucose and sialic acid contents in mucin glycoprotein through regulation of these enzyme activities, and inhibition of both Muc5ac mRNA and protein expressions in SO2-exposed rats.
KW - Carbocisteine
KW - Fucosidase
KW - Fucosyltransferase
KW - Muc5ac
KW - Sialidase
KW - Sialyltransferase
UR - http://www.scopus.com/inward/record.url?scp=1642277078&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1642277078&partnerID=8YFLogxK
U2 - 10.1016/j.ejphar.2003.12.038
DO - 10.1016/j.ejphar.2003.12.038
M3 - Article
C2 - 15033371
AN - SCOPUS:1642277078
VL - 487
SP - 7
EP - 15
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 1-3
ER -