Effects of CdSe/ZnS quantum dots covered multi-walled carbon nanotubes on murine embryonicstem cells

Daxiang Cui, Hong Zhang, Jie Sheng, Zheng Wang, Toru Asahi, Rong He, Tetsuya Osaka, Feng Gao, Hoon Sung Cho, Chris Huth, Hengyao Hu, Giovanni M. Pauletti, Donglu Shi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Stem cells nanotechnology has emerged as a new exciting area, and holds great potential for research and development of stem cells as novel therapeutic platforms for genetic, traumatic, and degenerative medicine. Vital to the success of this technology are approaches that reproducibly facilitate in vivo cell tracking, expansion, differentiation, and transplantation. Herein we reported the effects of CdSe/ZnS quantum dots covered multi-walled carbon nanotubes (FMNTs) on mice embryonic stem cell line CCE cells. The FMNTs were prepared by plasma surface treatment and characterized by high resolution transmission electron microscopy (HR-TEM), and incubated with murine ES CCE cells for 1 to 28 day.These ES cells were observed by confocal laser scanning microscopy, and were analyzed by real time reverse transcription-polymerase chain reaction (RTPCR), flow cytometry (FCM) and MTT method. Results showed that prepared FMNTs exhibited green fluorescent signal, could enter into ES cells in time-dependent means, more than 20 μg ml-1 FMNTs induced ES cells become smaller and smaller as the incubation time increased, and inhibited cell growth in dose-and time-dependent means, induced apoptosis of ES cells; conversely, 5 μg ml-1 FMNTs could markedly stimulate the expression of Sox1 and Hsp27, and inhibit expression of OCT4 in ES cells, FCM analysis showed that differentiation marker Flk-1 exhibited higher expression compared with control ES cells. In conclusion, high dose of FMNTs can inhibit proliferation of ES cells, low dose of FMNTs can improve the differentiation of ES cells, FMNTs can have potential applications in in vivo tracking, imaging and regulation of the proliferation and differentiation of ES cells.

Original languageEnglish
Pages (from-to)236-244
Number of pages9
JournalNano Biomedicine and Engineering
Volume2
Issue number4
Publication statusPublished - 2010

Fingerprint

Stem cells
Semiconductor quantum dots
Carbon nanotubes
Flow cytometry
Polymerase chain reaction
Cell growth
Cell death
Transcription
High resolution transmission electron microscopy
Nanotechnology
Medicine
Surface treatment
Microscopic examination
Scanning
Plasmas
Imaging techniques
Lasers

Keywords

  • Apoptosis
  • Cdse/zns quantum dots
  • Differentiation
  • Multi-walled carbon nanotubes
  • Murine embryonic stem cells
  • Proliferation

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Effects of CdSe/ZnS quantum dots covered multi-walled carbon nanotubes on murine embryonicstem cells. / Cui, Daxiang; Zhang, Hong; Sheng, Jie; Wang, Zheng; Asahi, Toru; He, Rong; Osaka, Tetsuya; Gao, Feng; Cho, Hoon Sung; Huth, Chris; Hu, Hengyao; Pauletti, Giovanni M.; Shi, Donglu.

In: Nano Biomedicine and Engineering, Vol. 2, No. 4, 2010, p. 236-244.

Research output: Contribution to journalArticle

Cui, D, Zhang, H, Sheng, J, Wang, Z, Asahi, T, He, R, Osaka, T, Gao, F, Cho, HS, Huth, C, Hu, H, Pauletti, GM & Shi, D 2010, 'Effects of CdSe/ZnS quantum dots covered multi-walled carbon nanotubes on murine embryonicstem cells', Nano Biomedicine and Engineering, vol. 2, no. 4, pp. 236-244.
Cui, Daxiang ; Zhang, Hong ; Sheng, Jie ; Wang, Zheng ; Asahi, Toru ; He, Rong ; Osaka, Tetsuya ; Gao, Feng ; Cho, Hoon Sung ; Huth, Chris ; Hu, Hengyao ; Pauletti, Giovanni M. ; Shi, Donglu. / Effects of CdSe/ZnS quantum dots covered multi-walled carbon nanotubes on murine embryonicstem cells. In: Nano Biomedicine and Engineering. 2010 ; Vol. 2, No. 4. pp. 236-244.
@article{d53f3eb3d56745b39b029ec9b10be560,
title = "Effects of CdSe/ZnS quantum dots covered multi-walled carbon nanotubes on murine embryonicstem cells",
abstract = "Stem cells nanotechnology has emerged as a new exciting area, and holds great potential for research and development of stem cells as novel therapeutic platforms for genetic, traumatic, and degenerative medicine. Vital to the success of this technology are approaches that reproducibly facilitate in vivo cell tracking, expansion, differentiation, and transplantation. Herein we reported the effects of CdSe/ZnS quantum dots covered multi-walled carbon nanotubes (FMNTs) on mice embryonic stem cell line CCE cells. The FMNTs were prepared by plasma surface treatment and characterized by high resolution transmission electron microscopy (HR-TEM), and incubated with murine ES CCE cells for 1 to 28 day.These ES cells were observed by confocal laser scanning microscopy, and were analyzed by real time reverse transcription-polymerase chain reaction (RTPCR), flow cytometry (FCM) and MTT method. Results showed that prepared FMNTs exhibited green fluorescent signal, could enter into ES cells in time-dependent means, more than 20 μg ml-1 FMNTs induced ES cells become smaller and smaller as the incubation time increased, and inhibited cell growth in dose-and time-dependent means, induced apoptosis of ES cells; conversely, 5 μg ml-1 FMNTs could markedly stimulate the expression of Sox1 and Hsp27, and inhibit expression of OCT4 in ES cells, FCM analysis showed that differentiation marker Flk-1 exhibited higher expression compared with control ES cells. In conclusion, high dose of FMNTs can inhibit proliferation of ES cells, low dose of FMNTs can improve the differentiation of ES cells, FMNTs can have potential applications in in vivo tracking, imaging and regulation of the proliferation and differentiation of ES cells.",
keywords = "Apoptosis, Cdse/zns quantum dots, Differentiation, Multi-walled carbon nanotubes, Murine embryonic stem cells, Proliferation",
author = "Daxiang Cui and Hong Zhang and Jie Sheng and Zheng Wang and Toru Asahi and Rong He and Tetsuya Osaka and Feng Gao and Cho, {Hoon Sung} and Chris Huth and Hengyao Hu and Pauletti, {Giovanni M.} and Donglu Shi",
year = "2010",
language = "English",
volume = "2",
pages = "236--244",
journal = "Nano Biomedicine and Engineering",
issn = "2150-5578",
publisher = "Open Access House of Science and Technology (OAHOST)",
number = "4",

}

TY - JOUR

T1 - Effects of CdSe/ZnS quantum dots covered multi-walled carbon nanotubes on murine embryonicstem cells

AU - Cui, Daxiang

AU - Zhang, Hong

AU - Sheng, Jie

AU - Wang, Zheng

AU - Asahi, Toru

AU - He, Rong

AU - Osaka, Tetsuya

AU - Gao, Feng

AU - Cho, Hoon Sung

AU - Huth, Chris

AU - Hu, Hengyao

AU - Pauletti, Giovanni M.

AU - Shi, Donglu

PY - 2010

Y1 - 2010

N2 - Stem cells nanotechnology has emerged as a new exciting area, and holds great potential for research and development of stem cells as novel therapeutic platforms for genetic, traumatic, and degenerative medicine. Vital to the success of this technology are approaches that reproducibly facilitate in vivo cell tracking, expansion, differentiation, and transplantation. Herein we reported the effects of CdSe/ZnS quantum dots covered multi-walled carbon nanotubes (FMNTs) on mice embryonic stem cell line CCE cells. The FMNTs were prepared by plasma surface treatment and characterized by high resolution transmission electron microscopy (HR-TEM), and incubated with murine ES CCE cells for 1 to 28 day.These ES cells were observed by confocal laser scanning microscopy, and were analyzed by real time reverse transcription-polymerase chain reaction (RTPCR), flow cytometry (FCM) and MTT method. Results showed that prepared FMNTs exhibited green fluorescent signal, could enter into ES cells in time-dependent means, more than 20 μg ml-1 FMNTs induced ES cells become smaller and smaller as the incubation time increased, and inhibited cell growth in dose-and time-dependent means, induced apoptosis of ES cells; conversely, 5 μg ml-1 FMNTs could markedly stimulate the expression of Sox1 and Hsp27, and inhibit expression of OCT4 in ES cells, FCM analysis showed that differentiation marker Flk-1 exhibited higher expression compared with control ES cells. In conclusion, high dose of FMNTs can inhibit proliferation of ES cells, low dose of FMNTs can improve the differentiation of ES cells, FMNTs can have potential applications in in vivo tracking, imaging and regulation of the proliferation and differentiation of ES cells.

AB - Stem cells nanotechnology has emerged as a new exciting area, and holds great potential for research and development of stem cells as novel therapeutic platforms for genetic, traumatic, and degenerative medicine. Vital to the success of this technology are approaches that reproducibly facilitate in vivo cell tracking, expansion, differentiation, and transplantation. Herein we reported the effects of CdSe/ZnS quantum dots covered multi-walled carbon nanotubes (FMNTs) on mice embryonic stem cell line CCE cells. The FMNTs were prepared by plasma surface treatment and characterized by high resolution transmission electron microscopy (HR-TEM), and incubated with murine ES CCE cells for 1 to 28 day.These ES cells were observed by confocal laser scanning microscopy, and were analyzed by real time reverse transcription-polymerase chain reaction (RTPCR), flow cytometry (FCM) and MTT method. Results showed that prepared FMNTs exhibited green fluorescent signal, could enter into ES cells in time-dependent means, more than 20 μg ml-1 FMNTs induced ES cells become smaller and smaller as the incubation time increased, and inhibited cell growth in dose-and time-dependent means, induced apoptosis of ES cells; conversely, 5 μg ml-1 FMNTs could markedly stimulate the expression of Sox1 and Hsp27, and inhibit expression of OCT4 in ES cells, FCM analysis showed that differentiation marker Flk-1 exhibited higher expression compared with control ES cells. In conclusion, high dose of FMNTs can inhibit proliferation of ES cells, low dose of FMNTs can improve the differentiation of ES cells, FMNTs can have potential applications in in vivo tracking, imaging and regulation of the proliferation and differentiation of ES cells.

KW - Apoptosis

KW - Cdse/zns quantum dots

KW - Differentiation

KW - Multi-walled carbon nanotubes

KW - Murine embryonic stem cells

KW - Proliferation

UR - http://www.scopus.com/inward/record.url?scp=80053234676&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80053234676&partnerID=8YFLogxK

M3 - Article

VL - 2

SP - 236

EP - 244

JO - Nano Biomedicine and Engineering

JF - Nano Biomedicine and Engineering

SN - 2150-5578

IS - 4

ER -