Effects of dendrimer-functionalized multi-walled carbon nanotubes on murine embryonic stem cells

D. Cui, H. Zhang, Z. Wang, Toru Asahi, Tetsuya Osaka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

14 Citations (Scopus)

Abstract

Herein we firstly reported the effects of generation 5 polyamidoamine dendrimerfunctionalized fluorescent multi-walled carbon nanotubes(dMNTs) on mice embryonic stem cell line CCE. The dMNTs were prepared and characterized, and incubated with murine ES CCE cells for 1 to 5day. These ES cells were observed by fluorescent microscopy, and were analyzed by flow cytometer and MTT. Results showed that the dendrimer-functionalized fluorescent multi-walled carbon nanotubes were successfully synthesized, could enter into ES cells quickly, more than 20μg/ml dose caused ES cells become smaller and smaller as the incubation time increased, and inhibited cell growth in dose- and time-dependent means, less than 5 μg/ml dose improves ES differentiation. These results demonstrate that dMNTs are toxic to ES cells at large dose, can induce ES cells' differentiation at small dose. The prepared dMNTs may be a highly efficient gene delivery system for ES cells, have potential applications in ES research.

Original languageEnglish
Title of host publicationECS Transactions
Pages111-116
Number of pages6
Volume13
Edition14
DOIs
Publication statusPublished - 2008
EventFullerenes, Nanotubes, and Carbon Nanostructures - 213th ECS Meeting - Phoenix, AZ, United States
Duration: 2008 May 182008 May 23

Other

OtherFullerenes, Nanotubes, and Carbon Nanostructures - 213th ECS Meeting
CountryUnited States
CityPhoenix, AZ
Period08/5/1808/5/23

Fingerprint

Dendrimers
Stem cells
Carbon nanotubes
Cell growth
Microscopic examination
Genes

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Effects of dendrimer-functionalized multi-walled carbon nanotubes on murine embryonic stem cells. / Cui, D.; Zhang, H.; Wang, Z.; Asahi, Toru; Osaka, Tetsuya.

ECS Transactions. Vol. 13 14. ed. 2008. p. 111-116.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Cui, D, Zhang, H, Wang, Z, Asahi, T & Osaka, T 2008, Effects of dendrimer-functionalized multi-walled carbon nanotubes on murine embryonic stem cells. in ECS Transactions. 14 edn, vol. 13, pp. 111-116, Fullerenes, Nanotubes, and Carbon Nanostructures - 213th ECS Meeting, Phoenix, AZ, United States, 08/5/18. https://doi.org/10.1149/1.2998536
Cui, D. ; Zhang, H. ; Wang, Z. ; Asahi, Toru ; Osaka, Tetsuya. / Effects of dendrimer-functionalized multi-walled carbon nanotubes on murine embryonic stem cells. ECS Transactions. Vol. 13 14. ed. 2008. pp. 111-116
@inproceedings{866d5f85ac3e4a26836b0abb1ef94646,
title = "Effects of dendrimer-functionalized multi-walled carbon nanotubes on murine embryonic stem cells",
abstract = "Herein we firstly reported the effects of generation 5 polyamidoamine dendrimerfunctionalized fluorescent multi-walled carbon nanotubes(dMNTs) on mice embryonic stem cell line CCE. The dMNTs were prepared and characterized, and incubated with murine ES CCE cells for 1 to 5day. These ES cells were observed by fluorescent microscopy, and were analyzed by flow cytometer and MTT. Results showed that the dendrimer-functionalized fluorescent multi-walled carbon nanotubes were successfully synthesized, could enter into ES cells quickly, more than 20μg/ml dose caused ES cells become smaller and smaller as the incubation time increased, and inhibited cell growth in dose- and time-dependent means, less than 5 μg/ml dose improves ES differentiation. These results demonstrate that dMNTs are toxic to ES cells at large dose, can induce ES cells' differentiation at small dose. The prepared dMNTs may be a highly efficient gene delivery system for ES cells, have potential applications in ES research.",
author = "D. Cui and H. Zhang and Z. Wang and Toru Asahi and Tetsuya Osaka",
year = "2008",
doi = "10.1149/1.2998536",
language = "English",
volume = "13",
pages = "111--116",
booktitle = "ECS Transactions",
edition = "14",

}

TY - GEN

T1 - Effects of dendrimer-functionalized multi-walled carbon nanotubes on murine embryonic stem cells

AU - Cui, D.

AU - Zhang, H.

AU - Wang, Z.

AU - Asahi, Toru

AU - Osaka, Tetsuya

PY - 2008

Y1 - 2008

N2 - Herein we firstly reported the effects of generation 5 polyamidoamine dendrimerfunctionalized fluorescent multi-walled carbon nanotubes(dMNTs) on mice embryonic stem cell line CCE. The dMNTs were prepared and characterized, and incubated with murine ES CCE cells for 1 to 5day. These ES cells were observed by fluorescent microscopy, and were analyzed by flow cytometer and MTT. Results showed that the dendrimer-functionalized fluorescent multi-walled carbon nanotubes were successfully synthesized, could enter into ES cells quickly, more than 20μg/ml dose caused ES cells become smaller and smaller as the incubation time increased, and inhibited cell growth in dose- and time-dependent means, less than 5 μg/ml dose improves ES differentiation. These results demonstrate that dMNTs are toxic to ES cells at large dose, can induce ES cells' differentiation at small dose. The prepared dMNTs may be a highly efficient gene delivery system for ES cells, have potential applications in ES research.

AB - Herein we firstly reported the effects of generation 5 polyamidoamine dendrimerfunctionalized fluorescent multi-walled carbon nanotubes(dMNTs) on mice embryonic stem cell line CCE. The dMNTs were prepared and characterized, and incubated with murine ES CCE cells for 1 to 5day. These ES cells were observed by fluorescent microscopy, and were analyzed by flow cytometer and MTT. Results showed that the dendrimer-functionalized fluorescent multi-walled carbon nanotubes were successfully synthesized, could enter into ES cells quickly, more than 20μg/ml dose caused ES cells become smaller and smaller as the incubation time increased, and inhibited cell growth in dose- and time-dependent means, less than 5 μg/ml dose improves ES differentiation. These results demonstrate that dMNTs are toxic to ES cells at large dose, can induce ES cells' differentiation at small dose. The prepared dMNTs may be a highly efficient gene delivery system for ES cells, have potential applications in ES research.

UR - http://www.scopus.com/inward/record.url?scp=65549159833&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65549159833&partnerID=8YFLogxK

U2 - 10.1149/1.2998536

DO - 10.1149/1.2998536

M3 - Conference contribution

VL - 13

SP - 111

EP - 116

BT - ECS Transactions

ER -