Effects of dendrimer-functionalized multi-walled carbon nanotubes on murine embryonic stem cells

D. Cui, H. Zhang, Z. Wang, A. Toru, O. Tetsuya

Research output: Contribution to journalConference article

15 Citations (Scopus)

Abstract

Herein we firstly reported the effects of generation 5 polyamidoamine dendrimerfunctionalized fluorescent multi-walled carbon nanotubes(dMNTs) on mice embryonic stem cell line CCE. The dMNTs were prepared and characterized, and incubated with murine ES CCE cells for 1 to 5day. These ES cells were observed by fluorescent microscopy, and were analyzed by flow cytometer and MTT. Results showed that the dendrimer-functionalized fluorescent multi-walled carbon nanotubes were successfully synthesized, could enter into ES cells quickly, more than 20μg/ml dose caused ES cells become smaller and smaller as the incubation time increased, and inhibited cell growth in dose- and time-dependent means, less than 5 μg/ml dose improves ES differentiation. These results demonstrate that dMNTs are toxic to ES cells at large dose, can induce ES cells' differentiation at small dose. The prepared dMNTs may be a highly efficient gene delivery system for ES cells, have potential applications in ES research.

Original languageEnglish
Pages (from-to)111-116
Number of pages6
JournalECS Transactions
Volume13
Issue number14
DOIs
Publication statusPublished - 2008 Dec 1
EventFullerenes, Nanotubes, and Carbon Nanostructures - 213th ECS Meeting - Phoenix, AZ, United States
Duration: 2008 May 182008 May 23

ASJC Scopus subject areas

  • Engineering(all)

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