Abstract
Herein we firstly reported the effects of generation 5 polyamidoamine dendrimerfunctionalized fluorescent multi-walled carbon nanotubes(dMNTs) on mice embryonic stem cell line CCE. The dMNTs were prepared and characterized, and incubated with murine ES CCE cells for 1 to 5day. These ES cells were observed by fluorescent microscopy, and were analyzed by flow cytometer and MTT. Results showed that the dendrimer-functionalized fluorescent multi-walled carbon nanotubes were successfully synthesized, could enter into ES cells quickly, more than 20μg/ml dose caused ES cells become smaller and smaller as the incubation time increased, and inhibited cell growth in dose- and time-dependent means, less than 5 μg/ml dose improves ES differentiation. These results demonstrate that dMNTs are toxic to ES cells at large dose, can induce ES cells' differentiation at small dose. The prepared dMNTs may be a highly efficient gene delivery system for ES cells, have potential applications in ES research.
| Original language | English |
|---|---|
| Title of host publication | ECS Transactions |
| Pages | 111-116 |
| Number of pages | 6 |
| Volume | 13 |
| Edition | 14 |
| DOIs | |
| Publication status | Published - 2008 |
| Event | Fullerenes, Nanotubes, and Carbon Nanostructures - 213th ECS Meeting - Phoenix, AZ, United States Duration: 2008 May 18 → 2008 May 23 |
Other
| Other | Fullerenes, Nanotubes, and Carbon Nanostructures - 213th ECS Meeting |
|---|---|
| Country | United States |
| City | Phoenix, AZ |
| Period | 08/5/18 → 08/5/23 |
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ASJC Scopus subject areas
- Engineering(all)
Cite this
Effects of dendrimer-functionalized multi-walled carbon nanotubes on murine embryonic stem cells. / Cui, D.; Zhang, H.; Wang, Z.; Asahi, Toru; Osaka, Tetsuya.
ECS Transactions. Vol. 13 14. ed. 2008. p. 111-116.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Effects of dendrimer-functionalized multi-walled carbon nanotubes on murine embryonic stem cells
AU - Cui, D.
AU - Zhang, H.
AU - Wang, Z.
AU - Asahi, Toru
AU - Osaka, Tetsuya
PY - 2008
Y1 - 2008
N2 - Herein we firstly reported the effects of generation 5 polyamidoamine dendrimerfunctionalized fluorescent multi-walled carbon nanotubes(dMNTs) on mice embryonic stem cell line CCE. The dMNTs were prepared and characterized, and incubated with murine ES CCE cells for 1 to 5day. These ES cells were observed by fluorescent microscopy, and were analyzed by flow cytometer and MTT. Results showed that the dendrimer-functionalized fluorescent multi-walled carbon nanotubes were successfully synthesized, could enter into ES cells quickly, more than 20μg/ml dose caused ES cells become smaller and smaller as the incubation time increased, and inhibited cell growth in dose- and time-dependent means, less than 5 μg/ml dose improves ES differentiation. These results demonstrate that dMNTs are toxic to ES cells at large dose, can induce ES cells' differentiation at small dose. The prepared dMNTs may be a highly efficient gene delivery system for ES cells, have potential applications in ES research.
AB - Herein we firstly reported the effects of generation 5 polyamidoamine dendrimerfunctionalized fluorescent multi-walled carbon nanotubes(dMNTs) on mice embryonic stem cell line CCE. The dMNTs were prepared and characterized, and incubated with murine ES CCE cells for 1 to 5day. These ES cells were observed by fluorescent microscopy, and were analyzed by flow cytometer and MTT. Results showed that the dendrimer-functionalized fluorescent multi-walled carbon nanotubes were successfully synthesized, could enter into ES cells quickly, more than 20μg/ml dose caused ES cells become smaller and smaller as the incubation time increased, and inhibited cell growth in dose- and time-dependent means, less than 5 μg/ml dose improves ES differentiation. These results demonstrate that dMNTs are toxic to ES cells at large dose, can induce ES cells' differentiation at small dose. The prepared dMNTs may be a highly efficient gene delivery system for ES cells, have potential applications in ES research.
UR - http://www.scopus.com/inward/record.url?scp=65549159833&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65549159833&partnerID=8YFLogxK
U2 - 10.1149/1.2998536
DO - 10.1149/1.2998536
M3 - Conference contribution
VL - 13
SP - 111
EP - 116
BT - ECS Transactions
ER -