Effects of diamond-FET-based RNA aptamer sensing for detection of real sample of HIV-1 Tat protein

A. Rahim Ruslinda, Kyosuke Tanabe, Shoji Ibori, Xianfen Wang, Hiroshi Kawarada

    Research output: Contribution to journalArticle

    42 Citations (Scopus)

    Abstract

    Diamond is a promising material for merging solid-state and biological systems owing to its chemical stability, low background current, wide potential window and biocompatibility. The effects of surface charge density on human immunodeficiency virus type 1 Trans-activator transcription (HIV-1 Tat) protein binding have been investigated on a diamond field-effect transistor (FET) using ribonucleic acid (RNA) aptamers as a sensing element on a solid surface. A change in the gate potential of 91.6. mV was observed, whereby a shift in the negative direction was observed at a source-drain current of -8. μA in the presence of HIV-1 Tat protein bound to the RNA aptamers. Moreover, the reversible change in gate potential caused by the binding and regeneration cycles was very stable throughout cyclical detections. The stable immobilization is achieved via RNA aptamers covalently bonded to the carboxyl-terminated terephtalic acids on amine sites, thereby increasing the sensitivity of the HIV-1 Tat protein sensor. The reliable use of a real sample of HIV-1 Tat protein by an aptamer-FET was demonstrated for the first time, which showed the potential of diamond biointerfaces in clinical biosensor applications.

    Original languageEnglish
    Pages (from-to)277-282
    Number of pages6
    JournalBiosensors and Bioelectronics
    Volume40
    Issue number1
    DOIs
    Publication statusPublished - 2013 Feb 15

    Fingerprint

    Diamond
    Trans-Activators
    Transcription
    Field effect transistors
    RNA
    Viruses
    HIV-1
    Diamonds
    Proteins
    Drain current
    Chemical stability
    Biosensing Techniques
    Biological systems
    Surface charge
    Charge density
    Biocompatibility
    Merging
    Protein Binding
    Biosensors
    Immobilization

    Keywords

    • Biosensor
    • Diamond FET
    • HIV-1 Tat protein
    • RNA aptamer

    ASJC Scopus subject areas

    • Biophysics
    • Biomedical Engineering
    • Biotechnology
    • Electrochemistry

    Cite this

    Effects of diamond-FET-based RNA aptamer sensing for detection of real sample of HIV-1 Tat protein. / Rahim Ruslinda, A.; Tanabe, Kyosuke; Ibori, Shoji; Wang, Xianfen; Kawarada, Hiroshi.

    In: Biosensors and Bioelectronics, Vol. 40, No. 1, 15.02.2013, p. 277-282.

    Research output: Contribution to journalArticle

    Rahim Ruslinda, A. ; Tanabe, Kyosuke ; Ibori, Shoji ; Wang, Xianfen ; Kawarada, Hiroshi. / Effects of diamond-FET-based RNA aptamer sensing for detection of real sample of HIV-1 Tat protein. In: Biosensors and Bioelectronics. 2013 ; Vol. 40, No. 1. pp. 277-282.
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