Effects of downstream genes on synthetic genetic circuits

Takefumi Moriya, Masayuki Yamamura, Daisuke Kiga

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: In order to understand and regulate complex genetic networks in living cells, it is important to build simple and well-defined genetic circuits. We designed such circuits using a synthetic biology approach that included mathematical modeling and simulation, with a focus on the effects by which downstream reporter genes are involved in the regulation of synthetic genetic circuits. Results: Our results indicated that downstream genes exert two main effects on genes involved in the regulation of synthetic genetic circuits: (1) competition for regulatory proteins and (2) protein degradation in the cell. Conclusions: Our findings regarding the effects of downstream genes on regulatory genes and the role of impedance in driving large-scale and complex genetic circuits may facilitate the design of more accurate genetic circuits. This design will have wide applications in future studies of systems and synthetic biology.

Original languageEnglish
Article numberS4
JournalBMC Systems Biology
Volume8
Issue number4
DOIs
Publication statusPublished - 2014 Dec 8
Externally publishedYes

Keywords

  • Decoy site
  • Genetic circuit
  • Impedance
  • Mathematical modeling
  • Protein degradation
  • Synthetic biology

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology
  • Applied Mathematics
  • Modelling and Simulation
  • Computer Science Applications
  • Medicine(all)

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