Effects of estrogen on AF64A-induced apoptosis in NG108-15 cells

Yusuke Kuwagata, Hiroko Inoue

    Research output: Contribution to journalArticle

    Abstract

    In this study, we show that pretreatment with physiological concentrations (1-100 nM) of 17β-estradiol decreased apoptosis induced by ethylcholine aziridinium (AF64A), a choline toxin, in the cholinergic neuronal cell line NG108-15. These protective effects were observed after short-term (30 min) pretreatment, and were blocked by treatment with an estrogen receptor antagonist and inhibitors of phosphatidylinositol 3-kinase (PI3K) and mitogen-activated protein kinase kinase (MEK). The protective effects were, however, not reversed by a protein synthesis inhibitor. Furthermore, we examined the effects of 17β-estradiol on choline uptake in NG108-15 cells. Although choline uptake was inhibited by a selective inhibitor of choline uptake, hemicholinium-3, it was not altered by treatment with 17β-estradiol. These results indicated that the protective effect of 17β-estradiol on AF64A-induced apoptosis could be nongenomic, and that this effect may be due to the activation of PI3K/Akt and/or MEK/extracellular signal-regulated kinase (ERK) pathways.

    Original languageEnglish
    Pages (from-to)9-16
    Number of pages8
    JournalBrain Research
    Volume1297
    DOIs
    Publication statusPublished - 2009 Oct 22

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    Keywords

    • 17β-Estradiol
    • AF64A
    • Apoptosis
    • Caspase-3
    • Protection

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Clinical Neurology
    • Developmental Biology
    • Molecular Biology

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