TY - JOUR
T1 - Effects of exercise training on growth and differentiation factor 11 expression in aged mice
AU - Lee, Minjung
AU - Oikawa, Satoshi
AU - Ushida, Takashi
AU - Suzuki, Katsuhiko
AU - Akimoto, Takayuki
N1 - Funding Information:
This study was supported, in part, by Grants-in Aid for Young Investigators (A) (Nos. 18680047 and 21680049 to TA) and Grants-in Aid for Scientific Research (B) (Nos. 25282198 and 16H03239 to TA) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. ML was supported by MEXT. SO was supported by the Japan Society for the Promotion of Science.
Publisher Copyright:
Copyright © 2019 Lee, Oikawa, Ushida, Suzuki and Akimoto. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
PY - 2019
Y1 - 2019
N2 - Exercise training is considered an effective way to prevent age-related skeletal muscle loss. However, the molecular mechanism has not been clarified. Growth and differentiation factor 11 (GDF11) has been controversially considered a regulator of skeletal muscle aging. In this study, we examined whether GDF11 is associated with skeletal muscle aging and the effects of exercise training on age-related skeletal muscle loss. First, we observed that Gdf11 mRNA and protein expression levels in young (5-month-old, n = 6) and aged (22-to 26-month-old, n = 5) mice were not significantly different. Aged mice were then divided into sedentary (n = 5) and exercise (n = 6) groups. The exercise group performed moderate-intensity treadmill running for 6 weeks. Treadmill exercise training increased Gdf11 mRNA expression in the soleus muscle, but its protein expression was not altered. In contrast, the GDF11 level in the plantaris muscle was not changed at either the mRNA or protein level. Collectively, our data demonstrate that GDF11 levels do not change during aging, and that treadmill exercise training increased Gdf11 mRNA expression in a predominantly slow-twitch muscle.
AB - Exercise training is considered an effective way to prevent age-related skeletal muscle loss. However, the molecular mechanism has not been clarified. Growth and differentiation factor 11 (GDF11) has been controversially considered a regulator of skeletal muscle aging. In this study, we examined whether GDF11 is associated with skeletal muscle aging and the effects of exercise training on age-related skeletal muscle loss. First, we observed that Gdf11 mRNA and protein expression levels in young (5-month-old, n = 6) and aged (22-to 26-month-old, n = 5) mice were not significantly different. Aged mice were then divided into sedentary (n = 5) and exercise (n = 6) groups. The exercise group performed moderate-intensity treadmill running for 6 weeks. Treadmill exercise training increased Gdf11 mRNA expression in the soleus muscle, but its protein expression was not altered. In contrast, the GDF11 level in the plantaris muscle was not changed at either the mRNA or protein level. Collectively, our data demonstrate that GDF11 levels do not change during aging, and that treadmill exercise training increased Gdf11 mRNA expression in a predominantly slow-twitch muscle.
KW - Aging
KW - Differentiation factor 11
KW - Exercise
KW - Growth
KW - Sarcopenia
KW - Skeletal muscle
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U2 - 10.3389/fphys.2019.00970
DO - 10.3389/fphys.2019.00970
M3 - Article
AN - SCOPUS:85070655274
VL - 10
JO - Frontiers in Physiology
JF - Frontiers in Physiology
SN - 1664-042X
IS - JUL
M1 - 970
ER -