Effects of experimental conditions on the mechanism of particle aggregation in protein precipitation by polyelectrolytes with a high molecular weight

Woon Soo Kim, Izumi Hirasawa, Woo Sik Kim

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    Lysozyme precipitation by polyacrylic acid (PAA), especially with a high molecular weight of 4 × 106, where the polymer bridging effect of PAA is thought to be dominant in the particle aggregation of lysozyme precipitates, was carried out to investigate the relationship between the bridging effect of PAA and the operation conditions in a semi-batch reactor. The bridging effect of PAA was found to be affected by the experimental conditions, such as the mixing intensity, PAA dosage, and ionic strength of the solution, which resulted in a variation in the particle size of the lysozyme precipitates due to a change in the aggregation mechanism. The polymer bridging of PAA was enhanced by increasing the agitation speed and PAA dosage and decreasing the ionic strength, which produced a larger particle size. However, under conditions of either poor mixing or small PAA dosage, smaller particles were formed with similar sizes, regardless of the ionic strength, as the bridging effect of PAA did not contribute to the precipitate aggregation. Based on the correlation between the concentration of the lysozyme precipitates and the mean particle size generated during the PAA feeding time, the point of change in the aggregation mechanism was identified and the precipitate concentration at which the mechanism conversion occurred was shorten by increasing the mixing intensity.

    Original languageEnglish
    Pages (from-to)6525-6534
    Number of pages10
    JournalChemical Engineering Science
    Volume56
    Issue number23
    DOIs
    Publication statusPublished - 2001 Nov 30

    Fingerprint

    carbopol 940
    Polyelectrolytes
    Agglomeration
    Molecular weight
    Proteins
    Acids
    Precipitates
    Muramidase
    Enzymes
    Ionic strength
    Particle size
    Polymers

    Keywords

    • Aggregation mechanism
    • Charge neutralization
    • Particle size
    • Polyelectrolyte precipitation
    • Polymer bridging
    • Power input

    ASJC Scopus subject areas

    • Chemical Engineering(all)

    Cite this

    Effects of experimental conditions on the mechanism of particle aggregation in protein precipitation by polyelectrolytes with a high molecular weight. / Kim, Woon Soo; Hirasawa, Izumi; Kim, Woo Sik.

    In: Chemical Engineering Science, Vol. 56, No. 23, 30.11.2001, p. 6525-6534.

    Research output: Contribution to journalArticle

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