Effects of Gaalas Diode Laser on Serum Opsonic Activity Assessed by Neutrophil-Associated Chemiluminescence

Vasile F. Dima, Katsuhiko Suzuki, Qiang Liu, Takuya Koie, Mutsuo Yamada, Koh Jun Suzuki, Shigeyuki Nakaji, Kazuo Sugawara

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The purpose of this study was to investigate the effects of low level laser therapy (LLLT: GaAlAs diode laser: 830 nm) on serum opsonic activity, which was assessed by neutrophil-associated chemiluminescence (CL) responses to zymosan opsonized with sera irradiated in vitro with various doses of LLL. We used both lucigenin-dependent CL (LgCL) for superoxide (O2 -) detection and luminol-dependent CL (LmCL) which detects myeloperoxidase (MPO)-dependent formation of hypochlorous acid in combination with an MPO inhibitor, sodium azide (NaN3). When serum opsonic activity was assessed by LgLC, NaN3 markedly enhanced the responses, suggesting that O2 - accumulates due to the MPO blockade, leading to the excitation of LgCL. However, LLL-irradiation had no effects on serum opsonic activity. On the other hand, when serum opsonic activity was assessed by LmCL, NaN3 strongly inhibited the response. The effects of LLLT at different output powers were characterized by similar values, but significantly higher values were observed at the highest dose tested (60 mW for 1 min) in the absence of NaN3. Since this enhancement effect disappeared in the presence of NaN3, it was suggested that high dose LLL-irradiation probably activates opsonic activity by facilitating the degranulation of MPO from neutrophils. However, lower doses similar to those used for therapeutic purposes had no effects at any output powers tested.

Original languageEnglish
Pages (from-to)153-158
Number of pages6
JournalLaser Therapy
Volume9
Issue number4
DOIs
Publication statusPublished - 1997 Jan 1
Externally publishedYes

Fingerprint

Semiconductor Lasers
Sodium Azide
Chemiluminescence
Luminescence
Semiconductor lasers
Neutrophils
Peroxidase
Serum
Irradiation
Luminol
Dosimetry
Sodium
Hypochlorous Acid
Zymosan
Acids
Lasers
Superoxides
Low-Level Light Therapy

Keywords

  • chemiluminescence
  • GaAlAs diode laser
  • lucigenin
  • luminol
  • myeloperoxidase
  • neutrophils
  • reactive oxygen species
  • serum opsonic activity

ASJC Scopus subject areas

  • Surgery
  • Biomedical Engineering

Cite this

Effects of Gaalas Diode Laser on Serum Opsonic Activity Assessed by Neutrophil-Associated Chemiluminescence. / Dima, Vasile F.; Suzuki, Katsuhiko; Liu, Qiang; Koie, Takuya; Yamada, Mutsuo; Suzuki, Koh Jun; Nakaji, Shigeyuki; Sugawara, Kazuo.

In: Laser Therapy, Vol. 9, No. 4, 01.01.1997, p. 153-158.

Research output: Contribution to journalArticle

Dima, VF, Suzuki, K, Liu, Q, Koie, T, Yamada, M, Suzuki, KJ, Nakaji, S & Sugawara, K 1997, 'Effects of Gaalas Diode Laser on Serum Opsonic Activity Assessed by Neutrophil-Associated Chemiluminescence', Laser Therapy, vol. 9, no. 4, pp. 153-158. https://doi.org/10.5978/islsm.9.153
Dima, Vasile F. ; Suzuki, Katsuhiko ; Liu, Qiang ; Koie, Takuya ; Yamada, Mutsuo ; Suzuki, Koh Jun ; Nakaji, Shigeyuki ; Sugawara, Kazuo. / Effects of Gaalas Diode Laser on Serum Opsonic Activity Assessed by Neutrophil-Associated Chemiluminescence. In: Laser Therapy. 1997 ; Vol. 9, No. 4. pp. 153-158.
@article{3edb176fcb2448e79a97240c45e4a5c1,
title = "Effects of Gaalas Diode Laser on Serum Opsonic Activity Assessed by Neutrophil-Associated Chemiluminescence",
abstract = "The purpose of this study was to investigate the effects of low level laser therapy (LLLT: GaAlAs diode laser: 830 nm) on serum opsonic activity, which was assessed by neutrophil-associated chemiluminescence (CL) responses to zymosan opsonized with sera irradiated in vitro with various doses of LLL. We used both lucigenin-dependent CL (LgCL) for superoxide (O2 -) detection and luminol-dependent CL (LmCL) which detects myeloperoxidase (MPO)-dependent formation of hypochlorous acid in combination with an MPO inhibitor, sodium azide (NaN3). When serum opsonic activity was assessed by LgLC, NaN3 markedly enhanced the responses, suggesting that O2 - accumulates due to the MPO blockade, leading to the excitation of LgCL. However, LLL-irradiation had no effects on serum opsonic activity. On the other hand, when serum opsonic activity was assessed by LmCL, NaN3 strongly inhibited the response. The effects of LLLT at different output powers were characterized by similar values, but significantly higher values were observed at the highest dose tested (60 mW for 1 min) in the absence of NaN3. Since this enhancement effect disappeared in the presence of NaN3, it was suggested that high dose LLL-irradiation probably activates opsonic activity by facilitating the degranulation of MPO from neutrophils. However, lower doses similar to those used for therapeutic purposes had no effects at any output powers tested.",
keywords = "chemiluminescence, GaAlAs diode laser, lucigenin, luminol, myeloperoxidase, neutrophils, reactive oxygen species, serum opsonic activity",
author = "Dima, {Vasile F.} and Katsuhiko Suzuki and Qiang Liu and Takuya Koie and Mutsuo Yamada and Suzuki, {Koh Jun} and Shigeyuki Nakaji and Kazuo Sugawara",
year = "1997",
month = "1",
day = "1",
doi = "10.5978/islsm.9.153",
language = "English",
volume = "9",
pages = "153--158",
journal = "Laser Therapy",
issn = "0898-5901",
publisher = "World Association for Laser Therapy",
number = "4",

}

TY - JOUR

T1 - Effects of Gaalas Diode Laser on Serum Opsonic Activity Assessed by Neutrophil-Associated Chemiluminescence

AU - Dima, Vasile F.

AU - Suzuki, Katsuhiko

AU - Liu, Qiang

AU - Koie, Takuya

AU - Yamada, Mutsuo

AU - Suzuki, Koh Jun

AU - Nakaji, Shigeyuki

AU - Sugawara, Kazuo

PY - 1997/1/1

Y1 - 1997/1/1

N2 - The purpose of this study was to investigate the effects of low level laser therapy (LLLT: GaAlAs diode laser: 830 nm) on serum opsonic activity, which was assessed by neutrophil-associated chemiluminescence (CL) responses to zymosan opsonized with sera irradiated in vitro with various doses of LLL. We used both lucigenin-dependent CL (LgCL) for superoxide (O2 -) detection and luminol-dependent CL (LmCL) which detects myeloperoxidase (MPO)-dependent formation of hypochlorous acid in combination with an MPO inhibitor, sodium azide (NaN3). When serum opsonic activity was assessed by LgLC, NaN3 markedly enhanced the responses, suggesting that O2 - accumulates due to the MPO blockade, leading to the excitation of LgCL. However, LLL-irradiation had no effects on serum opsonic activity. On the other hand, when serum opsonic activity was assessed by LmCL, NaN3 strongly inhibited the response. The effects of LLLT at different output powers were characterized by similar values, but significantly higher values were observed at the highest dose tested (60 mW for 1 min) in the absence of NaN3. Since this enhancement effect disappeared in the presence of NaN3, it was suggested that high dose LLL-irradiation probably activates opsonic activity by facilitating the degranulation of MPO from neutrophils. However, lower doses similar to those used for therapeutic purposes had no effects at any output powers tested.

AB - The purpose of this study was to investigate the effects of low level laser therapy (LLLT: GaAlAs diode laser: 830 nm) on serum opsonic activity, which was assessed by neutrophil-associated chemiluminescence (CL) responses to zymosan opsonized with sera irradiated in vitro with various doses of LLL. We used both lucigenin-dependent CL (LgCL) for superoxide (O2 -) detection and luminol-dependent CL (LmCL) which detects myeloperoxidase (MPO)-dependent formation of hypochlorous acid in combination with an MPO inhibitor, sodium azide (NaN3). When serum opsonic activity was assessed by LgLC, NaN3 markedly enhanced the responses, suggesting that O2 - accumulates due to the MPO blockade, leading to the excitation of LgCL. However, LLL-irradiation had no effects on serum opsonic activity. On the other hand, when serum opsonic activity was assessed by LmCL, NaN3 strongly inhibited the response. The effects of LLLT at different output powers were characterized by similar values, but significantly higher values were observed at the highest dose tested (60 mW for 1 min) in the absence of NaN3. Since this enhancement effect disappeared in the presence of NaN3, it was suggested that high dose LLL-irradiation probably activates opsonic activity by facilitating the degranulation of MPO from neutrophils. However, lower doses similar to those used for therapeutic purposes had no effects at any output powers tested.

KW - chemiluminescence

KW - GaAlAs diode laser

KW - lucigenin

KW - luminol

KW - myeloperoxidase

KW - neutrophils

KW - reactive oxygen species

KW - serum opsonic activity

UR - http://www.scopus.com/inward/record.url?scp=30944449153&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=30944449153&partnerID=8YFLogxK

U2 - 10.5978/islsm.9.153

DO - 10.5978/islsm.9.153

M3 - Article

VL - 9

SP - 153

EP - 158

JO - Laser Therapy

JF - Laser Therapy

SN - 0898-5901

IS - 4

ER -