Effects of hemoglobin vesicles, a liposomal artificial oxygen carrier, on hematological responses, complement and anaphylactic reactions in rats

Hideki Abe, Hiroshi Azuma, Miki Yamaguchi, Mitsuhiro Fujihara, Hisami Ikeda, Hiromi Sakai, Shinji Takeoka, Eishun Tsuchida

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    Hemoglobin vesicle (HbV), a liposomal oxygen carrier containing human hemoglobin, was intravenously infused into rats. After the infusion of saline, the HbV or empty vesicle (EV), numbers of red cells, leukocytes and platelets in peripheral blood were unchanged during the observation period of one week in addition to each time point among three groups. However, the lymphocyte ratio transiently decreased and the granulocyte ratio increased in the HbV and EV groups at 6 h after the infusion. Those changes returned to the initial value one day after the infusion and those were maintained for the subsequent observation period. No dramatic change was seen in the ratio of CD4 +/CD8+ T cells. A transient decrease of the complement titer was observed three days after the infusion of HbV and EV, although the consumption of complement titer was not detected in rat serum by mixing HbV or EV in vitro, indicating that the transient decrease of complement titer in vivo was not due to the consumption of complement due to the interaction with HbV or EV. Multiple infusions of HbV caused the decrease of complement titer only after the first infusion and no allergic reaction was observed. No anaphylactic shock was observed in rats administered with EV several times, while ovalbumin (OVA) sensitized rats died with symptoms of respiratory distress after the second OVA administration. These results indicate that HbV could be administered without serious clinical symptoms or adverse reactions.

    Original languageEnglish
    Pages (from-to)157-172
    Number of pages16
    JournalArtificial Cells, Blood Substitutes, and Biotechnology
    Volume35
    Issue number2
    DOIs
    Publication statusPublished - 2007 Mar

    Fingerprint

    Hemoglobin
    Anaphylaxis
    Rats
    Hemoglobins
    Oxygen
    Ovalbumin
    Observation
    CD4-CD8 Ratio
    T-cells
    Lymphocytes
    Platelets
    Platelet Count
    Granulocytes
    Hypersensitivity
    Leukocytes
    Blood
    Cell Count
    Cells
    T-Lymphocytes

    Keywords

    • Anaphylaxis
    • Complement
    • Hematological response
    • Hemoglobin vesicle
    • Liposome

    ASJC Scopus subject areas

    • Biotechnology
    • Biomedical Engineering
    • Hematology

    Cite this

    Effects of hemoglobin vesicles, a liposomal artificial oxygen carrier, on hematological responses, complement and anaphylactic reactions in rats. / Abe, Hideki; Azuma, Hiroshi; Yamaguchi, Miki; Fujihara, Mitsuhiro; Ikeda, Hisami; Sakai, Hiromi; Takeoka, Shinji; Tsuchida, Eishun.

    In: Artificial Cells, Blood Substitutes, and Biotechnology, Vol. 35, No. 2, 03.2007, p. 157-172.

    Research output: Contribution to journalArticle

    Abe, Hideki ; Azuma, Hiroshi ; Yamaguchi, Miki ; Fujihara, Mitsuhiro ; Ikeda, Hisami ; Sakai, Hiromi ; Takeoka, Shinji ; Tsuchida, Eishun. / Effects of hemoglobin vesicles, a liposomal artificial oxygen carrier, on hematological responses, complement and anaphylactic reactions in rats. In: Artificial Cells, Blood Substitutes, and Biotechnology. 2007 ; Vol. 35, No. 2. pp. 157-172.
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