Effects of high-intensity swimming training on GLUT-4 and glucose transport activity in rat skeletal muscle

Shin Terada, Toshiko Yokozeki, Kentaro Kawanaka, Kishiko Ogawa, Mitsuru Higuchi, Osamu Ezaki, Izumi Tabata

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

This study was performed to assess the effects of short-term, extremely high-intensity intermittent exercise training on the GLUT-4 content of rat skeletal muscle. Three- to fourweek-old male Sprague-Dawley rats with an initial body weight ranging from 45 to 55 g were used for this study. These rats were randomly assigned to an 8-day period of high-intensity intermittent exercise training (HIT), relatively high-intensity intermittent prolonged exercise training (RHT), or low-intensity prolonged exercise training (LIT). Age-matched sedentary rats were used as a control. In the HIT group, the rats repeated fourteen 20-s swimming bouts with a weight equivalent to 14, 15, and 16% of body weight for the first 2, the next 4, and the last 2 days, respectively. Between exercise bouts, a 10-s pause was allowed. RHT consisted of five 17-min swimming bouts with a 3-min rest between bouts. During the first bout, the rat swam without weight, whereas during the following four bouts, the rat was attached to a weight equivalent to 4 and 5% of its body weight for the first 5 days and the following 3 days, respectively. Rats in the LIT group swam 6 h/day for 8 days in two 3-h bouts separated by 45 min of rest. In the first experiment, the HIT, LIT, and control rats were compared. GLUT-4 content in the epitrochlearis muscle in the HIT and LIT groups after training was significantly higher than that in the control rats by 83 and 91%, respectively. Furthermore, glucose transport activity, stimulated maximally by both insulin (2 mU/ml) (HIT: 48%, LIT: 75%) and contractions (25. 10-s tetani) (HIT: 55%, LIT: 69%), was higher in the training groups than in the control rats. However, no significant differences in GLUT-4 content or in maximal glucose transport activity in response to both insulin and contractions were observed between the two training groups. The second experiment demonstrated that GLUT-4 content after HIT did not differ from that after RHT (66% higher in trained rats than in control). In conclusion, the present investigation demonstrated that 8 days of HIT lasting only 280 s elevated both GLUT-4 content and maximal glucose transport activity in rat skeletal muscle to a level similar to that attained after LIT, which has been considered a tool to increase GLUT-4 content maximally.

Original languageEnglish
Pages (from-to)2019-2024
Number of pages6
JournalJournal of Applied Physiology
Volume90
Issue number6
Publication statusPublished - 2001 Jun
Externally publishedYes

Fingerprint

Skeletal Muscle
Glucose
Exercise
Body Weight
Weights and Measures
High-Intensity Interval Training
Insulin
Tetanus
Sprague Dawley Rats
Muscles
Control Groups

Keywords

  • Epitrochlearis muscle
  • High-intensity exercise
  • Intermittent training

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Terada, S., Yokozeki, T., Kawanaka, K., Ogawa, K., Higuchi, M., Ezaki, O., & Tabata, I. (2001). Effects of high-intensity swimming training on GLUT-4 and glucose transport activity in rat skeletal muscle. Journal of Applied Physiology, 90(6), 2019-2024.

Effects of high-intensity swimming training on GLUT-4 and glucose transport activity in rat skeletal muscle. / Terada, Shin; Yokozeki, Toshiko; Kawanaka, Kentaro; Ogawa, Kishiko; Higuchi, Mitsuru; Ezaki, Osamu; Tabata, Izumi.

In: Journal of Applied Physiology, Vol. 90, No. 6, 06.2001, p. 2019-2024.

Research output: Contribution to journalArticle

Terada, S, Yokozeki, T, Kawanaka, K, Ogawa, K, Higuchi, M, Ezaki, O & Tabata, I 2001, 'Effects of high-intensity swimming training on GLUT-4 and glucose transport activity in rat skeletal muscle', Journal of Applied Physiology, vol. 90, no. 6, pp. 2019-2024.
Terada S, Yokozeki T, Kawanaka K, Ogawa K, Higuchi M, Ezaki O et al. Effects of high-intensity swimming training on GLUT-4 and glucose transport activity in rat skeletal muscle. Journal of Applied Physiology. 2001 Jun;90(6):2019-2024.
Terada, Shin ; Yokozeki, Toshiko ; Kawanaka, Kentaro ; Ogawa, Kishiko ; Higuchi, Mitsuru ; Ezaki, Osamu ; Tabata, Izumi. / Effects of high-intensity swimming training on GLUT-4 and glucose transport activity in rat skeletal muscle. In: Journal of Applied Physiology. 2001 ; Vol. 90, No. 6. pp. 2019-2024.
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