Effects of hydrogen bathing on exercise-induced oxidative stress and delayed-onset muscle soreness

Takuji Kawamura, Yuko Gando, Masaki Takahashi, Reira Hara, Katsuhiko Suzuki, Isao Muraoka

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4 Citations (Scopus)


Several studies have reported that molecular hydrogen (H2) acts as a therapeutic medical gas owing to scavenging reactive oxygen species (ROS). However, little is known about effects of H2 on exercise-induced oxidative stress. The purpose of this study was to investigate the effects of weekly hydrogen bathing on exercise-induced oxidative stress and delayed-onset muscle soreness (DOMS). Nine healthy and active young men participated in this study, and each subject performed hydrogen bathing trial and placebo bathing trial in a crossover design. The subjects performed downhill running (8 % decline) at 75 % peak oxygen uptake (VO2peak) for 30 min, and each subjects conducted hydrogen or placebo bathing for 20 min, respectively, 1-6 days after downhill running. Before and after exercise, we measured visual analogue scale (VAS) and collected blood samples (Pre-and 5 min, 60 min after the end of bathing, 1day, 2days, 3days, 7days after downhill running). Blood sample analyses include creatine kinase (CK), myoglobin (Mb), malondialdehyde (MDA), reactive oxygen metabolites (d-ROMs), biological antioxidant potential (BAP), myeloperoxidase (MPO), interleukin-6 (IL-6), interleukin-17a (IL-17a) and lactate concentrations. Weekly hydrogen bathing had no effects of exercise-induced oxidative stress and muscle damage. On the other hand, hydrogen bathing significantly reduced DOMS (VAS) 1 and 2days after downhill running (p=0.033). These findings suggest that hydrogen bath after downhill exercise can be effective for reduction of DOMS.

Original languageEnglish
Pages (from-to)297-305
Number of pages9
JournalJapanese Journal of Physical Fitness and Sports Medicine
Issue number3
Publication statusPublished - 2016



  • Bathing
  • Delayed-Onset Muscle Soreness
  • Downhill Running
  • Hydrogen (H)
  • Oxidative Stress

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

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