Effects of inhibitory and excitatory drugs on the metabolic rhythm of the hamster suprachiasmatic nucleus in vitro

Keiko Tominaga, Shigenobu Shibata, Showa Ueki, Shigenori Watanabe

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In order to elucidate the role of excitatory and inhibitory transmitters within the suprachiasmatic nucleus (SCN) in the circadian change of 2-deoxyglucose (2-DG) uptake in this nucleus, the effects of 8-hydroxy-2-(di-n-propylamino) tetralin hydrobromide (8-OH-DPAT), muscimol, flurazepam, pentobarbital and glutamate on uptake of 2-DG by hamster SCN were examined in hypothalamic slice preparations. 2-DG uptake in the SCN was high during the subjective day and low during the subjective night. The high uptake of 2-DG in the SCN during the daytime was inhibited by the superfusion of 8-OH-DPAT, muscimol, flurazepam and pentobarbital in a dose-dependent manner, but the low uptake of 2-DG during the night was unaffected. The low uptake during the night was significantly increased by treatment with glutamate, whereas 2-DG uptake during the day was unaffected. In contrast to the above results, 20 mM KCl and 1 μM tetrodotoxin increased and decreased 2-DG uptake during both the day and night, respectively. The present results strongly suggest that agonists of 5-HT1A receptors and GABAA-benzoadiazepine-barbiturate complex receptors regulate the function of the SCN through their inhibitory action on 2-DG uptake during the day, and that glutamate also regulates SCN function through it stimulatory action on 2-DG uptake during the night.

Original languageEnglish
Pages (from-to)79-84
Number of pages6
JournalEuropean Journal of Pharmacology
Volume217
Issue number1
DOIs
Publication statusPublished - 1992 Jun 24
Externally publishedYes

Keywords

  • 2-Deoxyglucose uptake
  • 8-OH-DPAT (8-hydroxy-2-(di-n-propylamino)tetralin)
  • Benzodiazepine receptors
  • Circadian rhythym
  • GABA receptors
  • Suprachiasmatic nucleus

ASJC Scopus subject areas

  • Pharmacology

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