Effects of lamprey PQRFamide peptides on brain gonadotropin-releasing hormone concentrations and pituitary gonadotropin-β mRNA expression

Dana Daukss, Kristen Gazda, Takayoshi Kosugi, Tomohiro Osugi, Kazuyoshi Tsutsui, Stacia A. Sower

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    Within the RFamide peptide family, PQRFamide peptides that include neuropeptide FF and AF possess a C-terminal Pro-Gln-Arg-Phe-NH 2 motif. We previously identified PQRFamide peptides, lamprey PQRFa, PQRFa-related peptide (RP)-1 and -RP-2 by immunoaffinity purification in the brain of lamprey, one of the most ancient vertebrate species [13]. Lamprey PQRFamide peptide precursor mRNA was expressed in regions predicted to be involved in neuroendocrine regulation in the hypothalamus. However, the putative function(s) of lamprey PQRFamide peptides (PQRFa, PQRFa-RP-1 and PQRFa-RP-2) were not examined nor was the distribution of PQRFamide peptides examined in other tissues besides the brain. The objective of this study was to determine tissue distribution of lamprey PQRFamide peptide precursor mRNA, and to examine the effects of PQRFamide peptides on brain gonadotropin-releasing hormone (GnRH)-I, -II, and -III protein concentrations, and pituitary gonadotropin (GTH)-β mRNA expression in adult lampreys. Lamprey PQRFamide peptide precursor mRNA was expressed in the eye and the brain. Lamprey PQRFa at 100μg/kg increased brain concentrations of lamprey GnRH-II compared with controls. PQRFa, PQRFa-RP-1 and PQRFa-RP-2 did not significantly change brain protein concentrations of either lamprey GnRH-I, -III, or lamprey GTH-β mRNA expression in the pituitary. These data suggest that one of the PQRFamide peptides may act as a neuroregulator of at least the lamprey GnRH-II system in adult female lamprey.

    Original languageEnglish
    Pages (from-to)215-219
    Number of pages5
    JournalGeneral and Comparative Endocrinology
    Volume177
    Issue number2
    DOIs
    Publication statusPublished - 2012 Jun 1

    Fingerprint

    pituitary gonadotropins
    Lampreys
    Pituitary Gonadotropins
    Petromyzontiformes
    gonadotropin-releasing hormone
    Gonadotropin-Releasing Hormone
    peptides
    brain
    Messenger RNA
    Peptides
    Brain
    RNA Precursors

    Keywords

    • GnRh
    • Gonadotropin
    • GTH beta
    • LPXRFamide peptides
    • Neuroendocrine regulation
    • PQRFamide peptides
    • RFamide peptides
    • Sea lampreys

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Effects of lamprey PQRFamide peptides on brain gonadotropin-releasing hormone concentrations and pituitary gonadotropin-β mRNA expression. / Daukss, Dana; Gazda, Kristen; Kosugi, Takayoshi; Osugi, Tomohiro; Tsutsui, Kazuyoshi; Sower, Stacia A.

    In: General and Comparative Endocrinology, Vol. 177, No. 2, 01.06.2012, p. 215-219.

    Research output: Contribution to journalArticle

    Daukss, Dana ; Gazda, Kristen ; Kosugi, Takayoshi ; Osugi, Tomohiro ; Tsutsui, Kazuyoshi ; Sower, Stacia A. / Effects of lamprey PQRFamide peptides on brain gonadotropin-releasing hormone concentrations and pituitary gonadotropin-β mRNA expression. In: General and Comparative Endocrinology. 2012 ; Vol. 177, No. 2. pp. 215-219.
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    AU - Gazda, Kristen

    AU - Kosugi, Takayoshi

    AU - Osugi, Tomohiro

    AU - Tsutsui, Kazuyoshi

    AU - Sower, Stacia A.

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    AB - Within the RFamide peptide family, PQRFamide peptides that include neuropeptide FF and AF possess a C-terminal Pro-Gln-Arg-Phe-NH 2 motif. We previously identified PQRFamide peptides, lamprey PQRFa, PQRFa-related peptide (RP)-1 and -RP-2 by immunoaffinity purification in the brain of lamprey, one of the most ancient vertebrate species [13]. Lamprey PQRFamide peptide precursor mRNA was expressed in regions predicted to be involved in neuroendocrine regulation in the hypothalamus. However, the putative function(s) of lamprey PQRFamide peptides (PQRFa, PQRFa-RP-1 and PQRFa-RP-2) were not examined nor was the distribution of PQRFamide peptides examined in other tissues besides the brain. The objective of this study was to determine tissue distribution of lamprey PQRFamide peptide precursor mRNA, and to examine the effects of PQRFamide peptides on brain gonadotropin-releasing hormone (GnRH)-I, -II, and -III protein concentrations, and pituitary gonadotropin (GTH)-β mRNA expression in adult lampreys. Lamprey PQRFamide peptide precursor mRNA was expressed in the eye and the brain. Lamprey PQRFa at 100μg/kg increased brain concentrations of lamprey GnRH-II compared with controls. PQRFa, PQRFa-RP-1 and PQRFa-RP-2 did not significantly change brain protein concentrations of either lamprey GnRH-I, -III, or lamprey GTH-β mRNA expression in the pituitary. These data suggest that one of the PQRFamide peptides may act as a neuroregulator of at least the lamprey GnRH-II system in adult female lamprey.

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    KW - Sea lampreys

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