Effects of Local Muscle Activity on Glut 4 Concentration in Skeletal Muscle

Kentaro Kawanaka, Mitsuru Higuchi, Shigeru Katsuta

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In the present study, we examined whether GLUT4 concentration in rat skeletal muscle is dependent on local muscle activity level or not. In ten male Sprague-Dawley rats, one side of gastrocnemius muscle w as tenotomized, and the other side contralateral muscle was treated sham operation as a control. Gastrocnemius and plantaris muscles were excised from both legs at the five weeks after surgery. After the muscles w ere weighed, GLUT 4 concentration and citrate synthase (CS) activity were measured. The results are summarized as follows:In gastrocnemius muscle, tenotomy induced decreases of 25% in muscle weight, 16% in CS activity, and 25% in GLUT 4 concentration as compared with the control muscle. These data suggest that although extramuscular environment is similar, different GLUT 4 concentration in both muscles is induced by different muscle activity level. Therefore, it is concluded that muscle activity level regulates GLUT 4 concentration in skeletal muscle. In overloaded synergistic plantaris muscle, muscle weight and GLUT 4 content per whole muscle were increased by 18% and 17%, respectively, but GLUT 4 concentration and CS activity were not changed as compared with the control muscle. These data could be interpreted that GLUT 4 concentration and mitochondrial oxidative enzyme activity in skeletal muscle are coregulated.

Original languageEnglish
Pages (from-to)389-395
Number of pages7
JournalJapanese Journal of Physical Fitness and Sports Medicine
Volume42
Issue number4
DOIs
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

Skeletal Muscle
Muscles
Citrate (si)-Synthase
Tenotomy
Weights and Measures
Sprague Dawley Rats
Leg
Enzymes

Keywords

  • gastrocnemius muscle
  • GLUT 4
  • local muscle activity
  • plantaris muscle
  • tenotomy

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Effects of Local Muscle Activity on Glut 4 Concentration in Skeletal Muscle. / Kawanaka, Kentaro; Higuchi, Mitsuru; Katsuta, Shigeru.

In: Japanese Journal of Physical Fitness and Sports Medicine, Vol. 42, No. 4, 1993, p. 389-395.

Research output: Contribution to journalArticle

Kawanaka, Kentaro ; Higuchi, Mitsuru ; Katsuta, Shigeru. / Effects of Local Muscle Activity on Glut 4 Concentration in Skeletal Muscle. In: Japanese Journal of Physical Fitness and Sports Medicine. 1993 ; Vol. 42, No. 4. pp. 389-395.
@article{643df28602d94aa4bd74ee9271870bc1,
title = "Effects of Local Muscle Activity on Glut 4 Concentration in Skeletal Muscle",
abstract = "In the present study, we examined whether GLUT4 concentration in rat skeletal muscle is dependent on local muscle activity level or not. In ten male Sprague-Dawley rats, one side of gastrocnemius muscle w as tenotomized, and the other side contralateral muscle was treated sham operation as a control. Gastrocnemius and plantaris muscles were excised from both legs at the five weeks after surgery. After the muscles w ere weighed, GLUT 4 concentration and citrate synthase (CS) activity were measured. The results are summarized as follows:In gastrocnemius muscle, tenotomy induced decreases of 25{\%} in muscle weight, 16{\%} in CS activity, and 25{\%} in GLUT 4 concentration as compared with the control muscle. These data suggest that although extramuscular environment is similar, different GLUT 4 concentration in both muscles is induced by different muscle activity level. Therefore, it is concluded that muscle activity level regulates GLUT 4 concentration in skeletal muscle. In overloaded synergistic plantaris muscle, muscle weight and GLUT 4 content per whole muscle were increased by 18{\%} and 17{\%}, respectively, but GLUT 4 concentration and CS activity were not changed as compared with the control muscle. These data could be interpreted that GLUT 4 concentration and mitochondrial oxidative enzyme activity in skeletal muscle are coregulated.",
keywords = "gastrocnemius muscle, GLUT 4, local muscle activity, plantaris muscle, tenotomy",
author = "Kentaro Kawanaka and Mitsuru Higuchi and Shigeru Katsuta",
year = "1993",
doi = "10.7600/jspfsm1949.42.389",
language = "English",
volume = "42",
pages = "389--395",
journal = "Japanese Journal of Physical Fitness and Sports Medicine",
issn = "0039-906X",
publisher = "Japanese Society of Physical Fitness and Sports Medicine",
number = "4",

}

TY - JOUR

T1 - Effects of Local Muscle Activity on Glut 4 Concentration in Skeletal Muscle

AU - Kawanaka, Kentaro

AU - Higuchi, Mitsuru

AU - Katsuta, Shigeru

PY - 1993

Y1 - 1993

N2 - In the present study, we examined whether GLUT4 concentration in rat skeletal muscle is dependent on local muscle activity level or not. In ten male Sprague-Dawley rats, one side of gastrocnemius muscle w as tenotomized, and the other side contralateral muscle was treated sham operation as a control. Gastrocnemius and plantaris muscles were excised from both legs at the five weeks after surgery. After the muscles w ere weighed, GLUT 4 concentration and citrate synthase (CS) activity were measured. The results are summarized as follows:In gastrocnemius muscle, tenotomy induced decreases of 25% in muscle weight, 16% in CS activity, and 25% in GLUT 4 concentration as compared with the control muscle. These data suggest that although extramuscular environment is similar, different GLUT 4 concentration in both muscles is induced by different muscle activity level. Therefore, it is concluded that muscle activity level regulates GLUT 4 concentration in skeletal muscle. In overloaded synergistic plantaris muscle, muscle weight and GLUT 4 content per whole muscle were increased by 18% and 17%, respectively, but GLUT 4 concentration and CS activity were not changed as compared with the control muscle. These data could be interpreted that GLUT 4 concentration and mitochondrial oxidative enzyme activity in skeletal muscle are coregulated.

AB - In the present study, we examined whether GLUT4 concentration in rat skeletal muscle is dependent on local muscle activity level or not. In ten male Sprague-Dawley rats, one side of gastrocnemius muscle w as tenotomized, and the other side contralateral muscle was treated sham operation as a control. Gastrocnemius and plantaris muscles were excised from both legs at the five weeks after surgery. After the muscles w ere weighed, GLUT 4 concentration and citrate synthase (CS) activity were measured. The results are summarized as follows:In gastrocnemius muscle, tenotomy induced decreases of 25% in muscle weight, 16% in CS activity, and 25% in GLUT 4 concentration as compared with the control muscle. These data suggest that although extramuscular environment is similar, different GLUT 4 concentration in both muscles is induced by different muscle activity level. Therefore, it is concluded that muscle activity level regulates GLUT 4 concentration in skeletal muscle. In overloaded synergistic plantaris muscle, muscle weight and GLUT 4 content per whole muscle were increased by 18% and 17%, respectively, but GLUT 4 concentration and CS activity were not changed as compared with the control muscle. These data could be interpreted that GLUT 4 concentration and mitochondrial oxidative enzyme activity in skeletal muscle are coregulated.

KW - gastrocnemius muscle

KW - GLUT 4

KW - local muscle activity

KW - plantaris muscle

KW - tenotomy

UR - http://www.scopus.com/inward/record.url?scp=85008096919&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85008096919&partnerID=8YFLogxK

U2 - 10.7600/jspfsm1949.42.389

DO - 10.7600/jspfsm1949.42.389

M3 - Article

VL - 42

SP - 389

EP - 395

JO - Japanese Journal of Physical Fitness and Sports Medicine

JF - Japanese Journal of Physical Fitness and Sports Medicine

SN - 0039-906X

IS - 4

ER -