Effects of neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) treatment on ovarian development of the sapphire devil, Chrysiptera cyanea

Muhammad Badruzzaman, Satoshi Imamura, Yuki Takeuchi, Taro Ikegami, Akihiro Takemura

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In the neuroendocrine system controlling fish reproduction, dopamine (DA) acts as a gonadotropin inhibitory factor and plays a role in regulating gonadal development of certain species. The present study examined the effects of chemical destruction of dopaminergic neurons in the brain on DA production and ovarian development in the sapphire devil Chrysiptera cyanea, a reef-associated damselfish. The avidin–biotin–peroxidase complex method using an antibody against tyrosine hydroxylase (TH), a critical enzyme in the DA synthesis pathway, identified a population of dopaminergic neurons with somata in the anteroventral preoptic nucleus of the diencephalon and fibers terminating in the proximal pars distalis of the pituitary. Maintaining fish in seawater containing 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) at 0.02 and 0.2 µg/mL for 2 days resulted in decreases in DA, 3,4-dihydroxyphenylacetic acid (DOPAC; DA metabolite), and DA metabolic rate in the whole brain. The number of TH-positive neurons in the diencephalon decreased after 0.02 µg/mL MPTP treatment for 2 days. These results suggest that MPTP treatment destroys TH-positive neurons in the diencephalon, thereby decreasing the synthesis and release of DA from the brain. This treatment rescued ovarian development in fish with artificially retracted ovaries during the spawning season. The gonadosomatic index of MPTP-treated fish 5 and 7 days after treatment was significantly higher than that of control fish. Oocytes in the vitellogenic stages were observed in the ovaries of MPTP-treated fish, but not in control fish. These results suggest that DA in the brain drives ovarian development in the sapphire devil.

Original languageEnglish
Pages (from-to)61-71
Number of pages11
JournalFish Physiology and Biochemistry
Volume41
Issue number1
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

sapphire
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
neurotoxins
ovarian development
Aluminum Oxide
Neurotoxins
dopamine
Dopamine
Fish
Fishes
tyrosine 3-monooxygenase
fish
brain
Diencephalon
Neurons
Brain
Tyrosine 3-Monooxygenase
neurons
3,4-Dihydroxyphenylacetic Acid
Dopaminergic Neurons

Keywords

  • Damselfish
  • Dopamine
  • MPTP
  • Ovary
  • Reproduction
  • Tyrosine hydroxylase
  • Vitellogenesis

ASJC Scopus subject areas

  • Aquatic Science
  • Biochemistry
  • Physiology

Cite this

Effects of neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) treatment on ovarian development of the sapphire devil, Chrysiptera cyanea. / Badruzzaman, Muhammad; Imamura, Satoshi; Takeuchi, Yuki; Ikegami, Taro; Takemura, Akihiro.

In: Fish Physiology and Biochemistry, Vol. 41, No. 1, 2014, p. 61-71.

Research output: Contribution to journalArticle

Badruzzaman, Muhammad ; Imamura, Satoshi ; Takeuchi, Yuki ; Ikegami, Taro ; Takemura, Akihiro. / Effects of neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) treatment on ovarian development of the sapphire devil, Chrysiptera cyanea. In: Fish Physiology and Biochemistry. 2014 ; Vol. 41, No. 1. pp. 61-71.
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AU - Takemura, Akihiro

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