Effects of nitric oxide synthase inhibition on fiber-type composition, mitochondrial biogenesis, and SIRT1 expression in rat skeletal muscle

Masataka Suwa, Hiroshi Nakano, Zsolt Radak, Shuzo Kumagai

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

It was hypothesized that nitric oxide synthases (NOS) regulated SIRT1 expression and lead to a corresponding changes of con-tractile and metabolic properties in skeletal muscle. The purpose of the present study was to investigate the influence of long-term inhibition of nitric oxide synthases (NOS) on the fiber-type composition, metabolic regulators such as and silent information regulator of transcription 1 (SIRT1) and peroxisome prolifera-tor-activated receptor γ coactivator-1α (PGC-1α), and compo-nents of mitochondrial biogenesis in the soleus and plantaris muscles of rats. Rats were assigned to two groups: control and NOS inhibitor (Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME), ingested for 8 weeks in drinking water)-treated groups. The percentage of Type I fibers in the L-NAME group was significantly lower than that in the control group, and the percentage of Type IIA fibers was concomitantly higher in soleus muscle. In plantaris muscle, muscle fiber composition was not altered by L-NAME treatment. L-NAME treatment decreased the cytochrome C protein expression and activity of mitochondrial oxidative enzymes in the plantaris muscle but not in soleus muscle. NOS inhibition reduced the SIRT1 protein expression level in both the soleus and plantaris muscles, whereas it did not affect the PGC-1α protein expression. L-NAME treatment also reduced the glucose transporter 4 protein expression in both muscles. These results suggest that NOS plays a role in maintaining SIRT1 protein expression, muscle fiber composition and components of mitochondrial biogenesis in skeletal muscle.

Original languageEnglish
Pages (from-to)548-555
Number of pages8
JournalJournal of Sports Science and Medicine
Volume14
Issue number3
Publication statusPublished - 2015 Sep 1
Externally publishedYes

Fingerprint

Organelle Biogenesis
Nitric Oxide Synthase
Skeletal Muscle
NG-Nitroarginine Methyl Ester
Muscles
Control Groups
Proteins
Peroxisomes
Facilitative Glucose Transport Proteins
Muscle Proteins
Cytochromes
Protein C
Drinking Water
Arginine

Keywords

  • Mitochondrial biogenesis
  • Muscle fiber composition
  • Nitric oxide
  • Nitric oxide synthase
  • Silent information regulator of transcription 1

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Effects of nitric oxide synthase inhibition on fiber-type composition, mitochondrial biogenesis, and SIRT1 expression in rat skeletal muscle. / Suwa, Masataka; Nakano, Hiroshi; Radak, Zsolt; Kumagai, Shuzo.

In: Journal of Sports Science and Medicine, Vol. 14, No. 3, 01.09.2015, p. 548-555.

Research output: Contribution to journalArticle

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