Effects of ovariectomy and prolactin on mammary gland expressions of transforming growth factor α and epidermal growth factor receptor mRNAs in mice

Hiroshi Nagasawa, Yoshie Ikezaki, Kazutoshi Yamamoto

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Beginning 15 days after ovariectomy (OVX), a high mammary tumor strain of SHN virgin mice at 3 months of age received subcutaneous injections of danazol (0.5 μg / 0.1 ml olive oil, once a day), perphenazine (0.05 mg / 0.1 ml saline, twice a day) or ovine prolactin (oPRL: 0.25 mg / 0.05 ml buffer, twice a day) for 3 days to modulate their circulating PRL levels. The serum PRL level was significantly decreased by danazol and increased by perphenazine compared to the intact and OVX-control groups. The expression of both transforming growth factor α (TGFα) mRNA and epidermal growth factor receptor (EGFR) mRNA in the mammary gland was increased by danazol. However, TGFα mRNA expression was decreased by perphenazine. Meanwhile, mammary end-bud formation was inhibited in danazol-treated group. All findings suggest that the manifestation of the effect of TGFα on mammary gland is rather suppressed by PRL, while mammary gland growth needs the participation of PRL; in other words, PRL is dominant to TGFα on the mammary gland growth. OVX resulted in a significant decrease of TGFα mRNA expression in the mammary gland despite of little alteration in serum PRL, confirming the previous observations. The similar trend was observed in ICR mice; however, the response to hormonal modulation is generally less susceptible than SHN mice.

    Original languageEnglish
    Pages (from-to)525-529
    Number of pages5
    JournalZoological Science
    Volume15
    Issue number4
    Publication statusPublished - 1998 Aug

    Fingerprint

    transforming growth factors
    ovariectomy
    prolactin
    mammary glands
    mice
    mammary neoplasms (animal)
    subcutaneous injection
    olive oil
    breasts
    buffers
    buds
    epidermal growth factor receptors
    sheep

    ASJC Scopus subject areas

    • Animal Science and Zoology

    Cite this

    Effects of ovariectomy and prolactin on mammary gland expressions of transforming growth factor α and epidermal growth factor receptor mRNAs in mice. / Nagasawa, Hiroshi; Ikezaki, Yoshie; Yamamoto, Kazutoshi.

    In: Zoological Science, Vol. 15, No. 4, 08.1998, p. 525-529.

    Research output: Contribution to journalArticle

    @article{44cc95e505a34c7fb774b66732a0df51,
    title = "Effects of ovariectomy and prolactin on mammary gland expressions of transforming growth factor α and epidermal growth factor receptor mRNAs in mice",
    abstract = "Beginning 15 days after ovariectomy (OVX), a high mammary tumor strain of SHN virgin mice at 3 months of age received subcutaneous injections of danazol (0.5 μg / 0.1 ml olive oil, once a day), perphenazine (0.05 mg / 0.1 ml saline, twice a day) or ovine prolactin (oPRL: 0.25 mg / 0.05 ml buffer, twice a day) for 3 days to modulate their circulating PRL levels. The serum PRL level was significantly decreased by danazol and increased by perphenazine compared to the intact and OVX-control groups. The expression of both transforming growth factor α (TGFα) mRNA and epidermal growth factor receptor (EGFR) mRNA in the mammary gland was increased by danazol. However, TGFα mRNA expression was decreased by perphenazine. Meanwhile, mammary end-bud formation was inhibited in danazol-treated group. All findings suggest that the manifestation of the effect of TGFα on mammary gland is rather suppressed by PRL, while mammary gland growth needs the participation of PRL; in other words, PRL is dominant to TGFα on the mammary gland growth. OVX resulted in a significant decrease of TGFα mRNA expression in the mammary gland despite of little alteration in serum PRL, confirming the previous observations. The similar trend was observed in ICR mice; however, the response to hormonal modulation is generally less susceptible than SHN mice.",
    author = "Hiroshi Nagasawa and Yoshie Ikezaki and Kazutoshi Yamamoto",
    year = "1998",
    month = "8",
    language = "English",
    volume = "15",
    pages = "525--529",
    journal = "Zoological Science",
    issn = "0289-0003",
    publisher = "Zoological Society of Japan",
    number = "4",

    }

    TY - JOUR

    T1 - Effects of ovariectomy and prolactin on mammary gland expressions of transforming growth factor α and epidermal growth factor receptor mRNAs in mice

    AU - Nagasawa, Hiroshi

    AU - Ikezaki, Yoshie

    AU - Yamamoto, Kazutoshi

    PY - 1998/8

    Y1 - 1998/8

    N2 - Beginning 15 days after ovariectomy (OVX), a high mammary tumor strain of SHN virgin mice at 3 months of age received subcutaneous injections of danazol (0.5 μg / 0.1 ml olive oil, once a day), perphenazine (0.05 mg / 0.1 ml saline, twice a day) or ovine prolactin (oPRL: 0.25 mg / 0.05 ml buffer, twice a day) for 3 days to modulate their circulating PRL levels. The serum PRL level was significantly decreased by danazol and increased by perphenazine compared to the intact and OVX-control groups. The expression of both transforming growth factor α (TGFα) mRNA and epidermal growth factor receptor (EGFR) mRNA in the mammary gland was increased by danazol. However, TGFα mRNA expression was decreased by perphenazine. Meanwhile, mammary end-bud formation was inhibited in danazol-treated group. All findings suggest that the manifestation of the effect of TGFα on mammary gland is rather suppressed by PRL, while mammary gland growth needs the participation of PRL; in other words, PRL is dominant to TGFα on the mammary gland growth. OVX resulted in a significant decrease of TGFα mRNA expression in the mammary gland despite of little alteration in serum PRL, confirming the previous observations. The similar trend was observed in ICR mice; however, the response to hormonal modulation is generally less susceptible than SHN mice.

    AB - Beginning 15 days after ovariectomy (OVX), a high mammary tumor strain of SHN virgin mice at 3 months of age received subcutaneous injections of danazol (0.5 μg / 0.1 ml olive oil, once a day), perphenazine (0.05 mg / 0.1 ml saline, twice a day) or ovine prolactin (oPRL: 0.25 mg / 0.05 ml buffer, twice a day) for 3 days to modulate their circulating PRL levels. The serum PRL level was significantly decreased by danazol and increased by perphenazine compared to the intact and OVX-control groups. The expression of both transforming growth factor α (TGFα) mRNA and epidermal growth factor receptor (EGFR) mRNA in the mammary gland was increased by danazol. However, TGFα mRNA expression was decreased by perphenazine. Meanwhile, mammary end-bud formation was inhibited in danazol-treated group. All findings suggest that the manifestation of the effect of TGFα on mammary gland is rather suppressed by PRL, while mammary gland growth needs the participation of PRL; in other words, PRL is dominant to TGFα on the mammary gland growth. OVX resulted in a significant decrease of TGFα mRNA expression in the mammary gland despite of little alteration in serum PRL, confirming the previous observations. The similar trend was observed in ICR mice; however, the response to hormonal modulation is generally less susceptible than SHN mice.

    UR - http://www.scopus.com/inward/record.url?scp=0041125882&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0041125882&partnerID=8YFLogxK

    M3 - Article

    VL - 15

    SP - 525

    EP - 529

    JO - Zoological Science

    JF - Zoological Science

    SN - 0289-0003

    IS - 4

    ER -