Effects of photodynamic therapy with talaporfin sodium on squamous cell carcinoma and sarcoma cells

Tomohiro Osaki, Yuki Kawase, Hiroshi Iseki, Shinji Kishimoto, Soko Ikuta, Yoshihiro Muragaki, Masamichi Yamashita, Kazuo Azuma, Yusuke Murahata, Takeshi Tsuka, Norihiko Itoh, Tomohiro Imagawa, Yoshiharu Okamoto

    Research output: Contribution to journalArticle

    Abstract

    Background Photodynamic therapy (PDT) uses a photosensitizer and light to destroy abnormal cells. Talaporfin sodium (NPe6) is a second-generation photosensitizer. Methods We evaluated the toxic effects of different combinations of laser and NPe6 doses on squamous cell carcinoma (KLN205) and sarcoma (Meth A) cell lines. The cells were incubated with 0, 5, 10, or 30 μg/mL NPe6 for 24 h. The cells were then irradiated with 0, 5, 15, or 30 mW/cm2 of laser power, and 0, 1, 5, 10, or 20 J/cm2 of laser energy. Cell viability was evaluated after 24 h. We also evaluated the cytotoxic effects of continuous wave or square-wave modulated laser irradiations (2, 5, or 10 Hz, 50% duty) on Meth A cells. Results The median lethal doses of NPe6 against the KLN205 and Meth A cells after treatment at a fluence rate of 15 mW/cm2 and a light dose of 20 J/cm2 were 18.6 and 5.0 μg/mL, respectively. Meth A cells were more sensitive to PDT than KLN205 cells. There was no significant difference between the effects of continuous wave and square-wave modulated lasers on Meth A cell viability. Conclusions NPe6 PDT induced cell death in a dose-dependent manner in KLN205 and Meth A cells. More work is required to evaluate the cytotoxic effects of square-wave modulated laser therapy at low light doses.

    Original languageEnglish
    Pages (from-to)213-220
    Number of pages8
    JournalPhotodiagnosis and Photodynamic Therapy
    Volume18
    DOIs
    Publication statusPublished - 2017 Jun 1

    Fingerprint

    Photochemotherapy
    Sarcoma
    Squamous Cell Carcinoma
    Lasers
    Photosensitizing Agents
    Light
    Cell Survival
    Talaporfin
    Poisons
    Laser Therapy
    Cell Death
    Cell Line

    Keywords

    • Continuous wave laser
    • Photodynamic therapy
    • Sarcoma
    • Squamous cell carcinoma
    • Square-wave modulated laser
    • Talaporfin sodium

    ASJC Scopus subject areas

    • Biophysics
    • Oncology
    • Dermatology
    • Pharmacology (medical)

    Cite this

    Effects of photodynamic therapy with talaporfin sodium on squamous cell carcinoma and sarcoma cells. / Osaki, Tomohiro; Kawase, Yuki; Iseki, Hiroshi; Kishimoto, Shinji; Ikuta, Soko; Muragaki, Yoshihiro; Yamashita, Masamichi; Azuma, Kazuo; Murahata, Yusuke; Tsuka, Takeshi; Itoh, Norihiko; Imagawa, Tomohiro; Okamoto, Yoshiharu.

    In: Photodiagnosis and Photodynamic Therapy, Vol. 18, 01.06.2017, p. 213-220.

    Research output: Contribution to journalArticle

    Osaki, T, Kawase, Y, Iseki, H, Kishimoto, S, Ikuta, S, Muragaki, Y, Yamashita, M, Azuma, K, Murahata, Y, Tsuka, T, Itoh, N, Imagawa, T & Okamoto, Y 2017, 'Effects of photodynamic therapy with talaporfin sodium on squamous cell carcinoma and sarcoma cells' Photodiagnosis and Photodynamic Therapy, vol. 18, pp. 213-220. https://doi.org/10.1016/j.pdpdt.2017.02.007
    Osaki, Tomohiro ; Kawase, Yuki ; Iseki, Hiroshi ; Kishimoto, Shinji ; Ikuta, Soko ; Muragaki, Yoshihiro ; Yamashita, Masamichi ; Azuma, Kazuo ; Murahata, Yusuke ; Tsuka, Takeshi ; Itoh, Norihiko ; Imagawa, Tomohiro ; Okamoto, Yoshiharu. / Effects of photodynamic therapy with talaporfin sodium on squamous cell carcinoma and sarcoma cells. In: Photodiagnosis and Photodynamic Therapy. 2017 ; Vol. 18. pp. 213-220.
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    abstract = "Background Photodynamic therapy (PDT) uses a photosensitizer and light to destroy abnormal cells. Talaporfin sodium (NPe6) is a second-generation photosensitizer. Methods We evaluated the toxic effects of different combinations of laser and NPe6 doses on squamous cell carcinoma (KLN205) and sarcoma (Meth A) cell lines. The cells were incubated with 0, 5, 10, or 30 μg/mL NPe6 for 24 h. The cells were then irradiated with 0, 5, 15, or 30 mW/cm2 of laser power, and 0, 1, 5, 10, or 20 J/cm2 of laser energy. Cell viability was evaluated after 24 h. We also evaluated the cytotoxic effects of continuous wave or square-wave modulated laser irradiations (2, 5, or 10 Hz, 50{\%} duty) on Meth A cells. Results The median lethal doses of NPe6 against the KLN205 and Meth A cells after treatment at a fluence rate of 15 mW/cm2 and a light dose of 20 J/cm2 were 18.6 and 5.0 μg/mL, respectively. Meth A cells were more sensitive to PDT than KLN205 cells. There was no significant difference between the effects of continuous wave and square-wave modulated lasers on Meth A cell viability. Conclusions NPe6 PDT induced cell death in a dose-dependent manner in KLN205 and Meth A cells. More work is required to evaluate the cytotoxic effects of square-wave modulated laser therapy at low light doses.",
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    author = "Tomohiro Osaki and Yuki Kawase and Hiroshi Iseki and Shinji Kishimoto and Soko Ikuta and Yoshihiro Muragaki and Masamichi Yamashita and Kazuo Azuma and Yusuke Murahata and Takeshi Tsuka and Norihiko Itoh and Tomohiro Imagawa and Yoshiharu Okamoto",
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    T1 - Effects of photodynamic therapy with talaporfin sodium on squamous cell carcinoma and sarcoma cells

    AU - Osaki, Tomohiro

    AU - Kawase, Yuki

    AU - Iseki, Hiroshi

    AU - Kishimoto, Shinji

    AU - Ikuta, Soko

    AU - Muragaki, Yoshihiro

    AU - Yamashita, Masamichi

    AU - Azuma, Kazuo

    AU - Murahata, Yusuke

    AU - Tsuka, Takeshi

    AU - Itoh, Norihiko

    AU - Imagawa, Tomohiro

    AU - Okamoto, Yoshiharu

    PY - 2017/6/1

    Y1 - 2017/6/1

    N2 - Background Photodynamic therapy (PDT) uses a photosensitizer and light to destroy abnormal cells. Talaporfin sodium (NPe6) is a second-generation photosensitizer. Methods We evaluated the toxic effects of different combinations of laser and NPe6 doses on squamous cell carcinoma (KLN205) and sarcoma (Meth A) cell lines. The cells were incubated with 0, 5, 10, or 30 μg/mL NPe6 for 24 h. The cells were then irradiated with 0, 5, 15, or 30 mW/cm2 of laser power, and 0, 1, 5, 10, or 20 J/cm2 of laser energy. Cell viability was evaluated after 24 h. We also evaluated the cytotoxic effects of continuous wave or square-wave modulated laser irradiations (2, 5, or 10 Hz, 50% duty) on Meth A cells. Results The median lethal doses of NPe6 against the KLN205 and Meth A cells after treatment at a fluence rate of 15 mW/cm2 and a light dose of 20 J/cm2 were 18.6 and 5.0 μg/mL, respectively. Meth A cells were more sensitive to PDT than KLN205 cells. There was no significant difference between the effects of continuous wave and square-wave modulated lasers on Meth A cell viability. Conclusions NPe6 PDT induced cell death in a dose-dependent manner in KLN205 and Meth A cells. More work is required to evaluate the cytotoxic effects of square-wave modulated laser therapy at low light doses.

    AB - Background Photodynamic therapy (PDT) uses a photosensitizer and light to destroy abnormal cells. Talaporfin sodium (NPe6) is a second-generation photosensitizer. Methods We evaluated the toxic effects of different combinations of laser and NPe6 doses on squamous cell carcinoma (KLN205) and sarcoma (Meth A) cell lines. The cells were incubated with 0, 5, 10, or 30 μg/mL NPe6 for 24 h. The cells were then irradiated with 0, 5, 15, or 30 mW/cm2 of laser power, and 0, 1, 5, 10, or 20 J/cm2 of laser energy. Cell viability was evaluated after 24 h. We also evaluated the cytotoxic effects of continuous wave or square-wave modulated laser irradiations (2, 5, or 10 Hz, 50% duty) on Meth A cells. Results The median lethal doses of NPe6 against the KLN205 and Meth A cells after treatment at a fluence rate of 15 mW/cm2 and a light dose of 20 J/cm2 were 18.6 and 5.0 μg/mL, respectively. Meth A cells were more sensitive to PDT than KLN205 cells. There was no significant difference between the effects of continuous wave and square-wave modulated lasers on Meth A cell viability. Conclusions NPe6 PDT induced cell death in a dose-dependent manner in KLN205 and Meth A cells. More work is required to evaluate the cytotoxic effects of square-wave modulated laser therapy at low light doses.

    KW - Continuous wave laser

    KW - Photodynamic therapy

    KW - Sarcoma

    KW - Squamous cell carcinoma

    KW - Square-wave modulated laser

    KW - Talaporfin sodium

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    U2 - 10.1016/j.pdpdt.2017.02.007

    DO - 10.1016/j.pdpdt.2017.02.007

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    SP - 213

    EP - 220

    JO - Photodiagnosis and Photodynamic Therapy

    JF - Photodiagnosis and Photodynamic Therapy

    SN - 1572-1000

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