Effects of photodynamic therapy with talaporfin sodium on squamous cell carcinoma and sarcoma cells

Tomohiro Osaki, Yuki Kawase, Hiroshi Iseki, Shinji Kishimoto, Soko Ikuta, Yoshihiro Muragaki, Masamichi Yamashita, Kazuo Azuma, Yusuke Murahata, Takeshi Tsuka, Norihiko Itoh, Tomohiro Imagawa, Yoshiharu Okamoto

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Background Photodynamic therapy (PDT) uses a photosensitizer and light to destroy abnormal cells. Talaporfin sodium (NPe6) is a second-generation photosensitizer. Methods We evaluated the toxic effects of different combinations of laser and NPe6 doses on squamous cell carcinoma (KLN205) and sarcoma (Meth A) cell lines. The cells were incubated with 0, 5, 10, or 30 μg/mL NPe6 for 24 h. The cells were then irradiated with 0, 5, 15, or 30 mW/cm2 of laser power, and 0, 1, 5, 10, or 20 J/cm2 of laser energy. Cell viability was evaluated after 24 h. We also evaluated the cytotoxic effects of continuous wave or square-wave modulated laser irradiations (2, 5, or 10 Hz, 50% duty) on Meth A cells. Results The median lethal doses of NPe6 against the KLN205 and Meth A cells after treatment at a fluence rate of 15 mW/cm2 and a light dose of 20 J/cm2 were 18.6 and 5.0 μg/mL, respectively. Meth A cells were more sensitive to PDT than KLN205 cells. There was no significant difference between the effects of continuous wave and square-wave modulated lasers on Meth A cell viability. Conclusions NPe6 PDT induced cell death in a dose-dependent manner in KLN205 and Meth A cells. More work is required to evaluate the cytotoxic effects of square-wave modulated laser therapy at low light doses.

    Original languageEnglish
    Pages (from-to)213-220
    Number of pages8
    JournalPhotodiagnosis and Photodynamic Therapy
    Volume18
    DOIs
    Publication statusPublished - 2017 Jun 1

    Keywords

    • Continuous wave laser
    • Photodynamic therapy
    • Sarcoma
    • Squamous cell carcinoma
    • Square-wave modulated laser
    • Talaporfin sodium

    ASJC Scopus subject areas

    • Biophysics
    • Oncology
    • Dermatology
    • Pharmacology (medical)

    Fingerprint Dive into the research topics of 'Effects of photodynamic therapy with talaporfin sodium on squamous cell carcinoma and sarcoma cells'. Together they form a unique fingerprint.

  • Cite this

    Osaki, T., Kawase, Y., Iseki, H., Kishimoto, S., Ikuta, S., Muragaki, Y., Yamashita, M., Azuma, K., Murahata, Y., Tsuka, T., Itoh, N., Imagawa, T., & Okamoto, Y. (2017). Effects of photodynamic therapy with talaporfin sodium on squamous cell carcinoma and sarcoma cells. Photodiagnosis and Photodynamic Therapy, 18, 213-220. https://doi.org/10.1016/j.pdpdt.2017.02.007