Effects of pituitary adenylate cyclase-activating polypeptide, vasoactive intestinal polypeptide, and somatostatin on the release of thyrotropin from the bullfrog pituitary

Reiko Okada, Kazutoshi Yamamoto, Yoichi Ito, Nicolas Chartrel, Jerome Leprince, Alain Fournter, Hubert Vaudry, Sakae Kikuyama

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    The recent development of a specific radioimmunoassay for amphibian (bullfrog, Rana catesbeiana) thyrotropin (TSH) has made it possible to study the effects of various neuropeptides on the release of TSH from the pituitary in vitro. Up to now, corticotropin-releasing factor of bullfrog origin has been shown to have a potent TSH-releasing activity, whereas gonadotropin-releasing hormone and TSH-releasing hormone exhibit a moderate TSH-releasing effect on the adult, but not larval, pituitary. In the present study, the effects of pituitary adenylate cyclase-activating polypeptide (PACAP), vasoactive intestinal polypeptide (VIP), and somatostatin (SS) on the in vitro release of TSH from the bullfrog pituitary were investigated. Both frog (R. ridibunda) PACAP-38 and PACAP-27 caused a concentration-dependent stimulation of the release of TSH from dispersed pituitary cells during a 24-h culture. The PACAP-38- and PACAP-27-induced TSH release was suppressed by a simultaneous application of PACAP6-38. Application of high concentrations of PACAP6-38 alone caused a slight but significant stimulatory effect on the release of TSH. Frog VIP also stimulated TSH release from pituitary cells concentration-dependently. Frog SS1 (homologous to mammalian somatostatin-14) and SS2 (homologous to mammalian cortistatin) did not affect the basal release of TSH but caused a concentration-dependent suppression of the PACAP-38-induced release of TSH. These results suggest the involvement of multiple neuropeptides in the regulation of the release of TSH from the amphibian pituitary.

    Original languageEnglish
    Pages (from-to)474-480
    Number of pages7
    JournalAnnals of the New York Academy of Sciences
    Volume1070
    DOIs
    Publication statusPublished - 2006 Jul

    Fingerprint

    Pituitary Adenylate Cyclase-Activating Polypeptide
    Rana catesbeiana
    Vasoactive Intestinal Peptide
    Thyrotropin
    Somatostatin
    Anura
    Amphibians
    Neuropeptides
    Corticotropin-Releasing Hormone
    Gonadotropin-Releasing Hormone
    Radioimmunoassay
    Hormones
    Frog
    Cells
    Amphibian

    Keywords

    • Amphibia
    • PACAP
    • Somatostatin
    • TSH
    • VIP

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

    Cite this

    Effects of pituitary adenylate cyclase-activating polypeptide, vasoactive intestinal polypeptide, and somatostatin on the release of thyrotropin from the bullfrog pituitary. / Okada, Reiko; Yamamoto, Kazutoshi; Ito, Yoichi; Chartrel, Nicolas; Leprince, Jerome; Fournter, Alain; Vaudry, Hubert; Kikuyama, Sakae.

    In: Annals of the New York Academy of Sciences, Vol. 1070, 07.2006, p. 474-480.

    Research output: Contribution to journalArticle

    Okada, Reiko ; Yamamoto, Kazutoshi ; Ito, Yoichi ; Chartrel, Nicolas ; Leprince, Jerome ; Fournter, Alain ; Vaudry, Hubert ; Kikuyama, Sakae. / Effects of pituitary adenylate cyclase-activating polypeptide, vasoactive intestinal polypeptide, and somatostatin on the release of thyrotropin from the bullfrog pituitary. In: Annals of the New York Academy of Sciences. 2006 ; Vol. 1070. pp. 474-480.
    @article{e2ba639612874f0ea4fe52efa97cb50d,
    title = "Effects of pituitary adenylate cyclase-activating polypeptide, vasoactive intestinal polypeptide, and somatostatin on the release of thyrotropin from the bullfrog pituitary",
    abstract = "The recent development of a specific radioimmunoassay for amphibian (bullfrog, Rana catesbeiana) thyrotropin (TSH) has made it possible to study the effects of various neuropeptides on the release of TSH from the pituitary in vitro. Up to now, corticotropin-releasing factor of bullfrog origin has been shown to have a potent TSH-releasing activity, whereas gonadotropin-releasing hormone and TSH-releasing hormone exhibit a moderate TSH-releasing effect on the adult, but not larval, pituitary. In the present study, the effects of pituitary adenylate cyclase-activating polypeptide (PACAP), vasoactive intestinal polypeptide (VIP), and somatostatin (SS) on the in vitro release of TSH from the bullfrog pituitary were investigated. Both frog (R. ridibunda) PACAP-38 and PACAP-27 caused a concentration-dependent stimulation of the release of TSH from dispersed pituitary cells during a 24-h culture. The PACAP-38- and PACAP-27-induced TSH release was suppressed by a simultaneous application of PACAP6-38. Application of high concentrations of PACAP6-38 alone caused a slight but significant stimulatory effect on the release of TSH. Frog VIP also stimulated TSH release from pituitary cells concentration-dependently. Frog SS1 (homologous to mammalian somatostatin-14) and SS2 (homologous to mammalian cortistatin) did not affect the basal release of TSH but caused a concentration-dependent suppression of the PACAP-38-induced release of TSH. These results suggest the involvement of multiple neuropeptides in the regulation of the release of TSH from the amphibian pituitary.",
    keywords = "Amphibia, PACAP, Somatostatin, TSH, VIP",
    author = "Reiko Okada and Kazutoshi Yamamoto and Yoichi Ito and Nicolas Chartrel and Jerome Leprince and Alain Fournter and Hubert Vaudry and Sakae Kikuyama",
    year = "2006",
    month = "7",
    doi = "10.1196/annals.1317.064",
    language = "English",
    volume = "1070",
    pages = "474--480",
    journal = "Annals of the New York Academy of Sciences",
    issn = "0077-8923",
    publisher = "Wiley-Blackwell",

    }

    TY - JOUR

    T1 - Effects of pituitary adenylate cyclase-activating polypeptide, vasoactive intestinal polypeptide, and somatostatin on the release of thyrotropin from the bullfrog pituitary

    AU - Okada, Reiko

    AU - Yamamoto, Kazutoshi

    AU - Ito, Yoichi

    AU - Chartrel, Nicolas

    AU - Leprince, Jerome

    AU - Fournter, Alain

    AU - Vaudry, Hubert

    AU - Kikuyama, Sakae

    PY - 2006/7

    Y1 - 2006/7

    N2 - The recent development of a specific radioimmunoassay for amphibian (bullfrog, Rana catesbeiana) thyrotropin (TSH) has made it possible to study the effects of various neuropeptides on the release of TSH from the pituitary in vitro. Up to now, corticotropin-releasing factor of bullfrog origin has been shown to have a potent TSH-releasing activity, whereas gonadotropin-releasing hormone and TSH-releasing hormone exhibit a moderate TSH-releasing effect on the adult, but not larval, pituitary. In the present study, the effects of pituitary adenylate cyclase-activating polypeptide (PACAP), vasoactive intestinal polypeptide (VIP), and somatostatin (SS) on the in vitro release of TSH from the bullfrog pituitary were investigated. Both frog (R. ridibunda) PACAP-38 and PACAP-27 caused a concentration-dependent stimulation of the release of TSH from dispersed pituitary cells during a 24-h culture. The PACAP-38- and PACAP-27-induced TSH release was suppressed by a simultaneous application of PACAP6-38. Application of high concentrations of PACAP6-38 alone caused a slight but significant stimulatory effect on the release of TSH. Frog VIP also stimulated TSH release from pituitary cells concentration-dependently. Frog SS1 (homologous to mammalian somatostatin-14) and SS2 (homologous to mammalian cortistatin) did not affect the basal release of TSH but caused a concentration-dependent suppression of the PACAP-38-induced release of TSH. These results suggest the involvement of multiple neuropeptides in the regulation of the release of TSH from the amphibian pituitary.

    AB - The recent development of a specific radioimmunoassay for amphibian (bullfrog, Rana catesbeiana) thyrotropin (TSH) has made it possible to study the effects of various neuropeptides on the release of TSH from the pituitary in vitro. Up to now, corticotropin-releasing factor of bullfrog origin has been shown to have a potent TSH-releasing activity, whereas gonadotropin-releasing hormone and TSH-releasing hormone exhibit a moderate TSH-releasing effect on the adult, but not larval, pituitary. In the present study, the effects of pituitary adenylate cyclase-activating polypeptide (PACAP), vasoactive intestinal polypeptide (VIP), and somatostatin (SS) on the in vitro release of TSH from the bullfrog pituitary were investigated. Both frog (R. ridibunda) PACAP-38 and PACAP-27 caused a concentration-dependent stimulation of the release of TSH from dispersed pituitary cells during a 24-h culture. The PACAP-38- and PACAP-27-induced TSH release was suppressed by a simultaneous application of PACAP6-38. Application of high concentrations of PACAP6-38 alone caused a slight but significant stimulatory effect on the release of TSH. Frog VIP also stimulated TSH release from pituitary cells concentration-dependently. Frog SS1 (homologous to mammalian somatostatin-14) and SS2 (homologous to mammalian cortistatin) did not affect the basal release of TSH but caused a concentration-dependent suppression of the PACAP-38-induced release of TSH. These results suggest the involvement of multiple neuropeptides in the regulation of the release of TSH from the amphibian pituitary.

    KW - Amphibia

    KW - PACAP

    KW - Somatostatin

    KW - TSH

    KW - VIP

    UR - http://www.scopus.com/inward/record.url?scp=33746452107&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=33746452107&partnerID=8YFLogxK

    U2 - 10.1196/annals.1317.064

    DO - 10.1196/annals.1317.064

    M3 - Article

    C2 - 16888212

    AN - SCOPUS:33746452107

    VL - 1070

    SP - 474

    EP - 480

    JO - Annals of the New York Academy of Sciences

    JF - Annals of the New York Academy of Sciences

    SN - 0077-8923

    ER -