Effects of polyamines on a continuous cell-free protein synthesis system of an extreme thermophile, Thermus thermophilus

Taketoshi Uzawa, Akihiko Yamagishi, Takuya Ueda, Nobutoshi Chikazumi, Kimitsuna Watanabe, Tairo Oshlma

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A continuous cell-free protein synthesis system of an extremely thermophilic eubacterium, Thermus thermophilus HB27, was constructed. This system produced MS2 phage RNA translation products at a rate of more than 5 μg per hour per 1.9 mg of ribosomes at 65°C and the production continued linearly for at least 340 min. When no polyamine was added, the system did not produce the proteins. The highest activity was recorded when 0.1 mM tetrakis(3-aminopropyl)ammonium and 1.0 mM spermine were added simultaneously.

Original languageEnglish
Pages (from-to)732-734
Number of pages3
JournalJournal of biochemistry
Volume114
Issue number5
DOIs
Publication statusPublished - 1993 Nov

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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