Effects of poly(Ethyleneglycol)-modified hemoglobin vesicles on agonist-induced platelet aggregation and rantes release in vitro

Shinobu Wakamoto, Mitsuhiro Fujihara, Hideki Abe, Hiromi Sakai, Shinji Takeoka, Eishun Tsuchida, Hisami Ikeda, Kenji Ikebuchi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We studied the effects of hemoglobin-vesicles modified with PEG (PEG-HbV), a type of liposome-encapsulated hemoglobin (LEH), on human platelet functions in vitro. The effect of a low concentration of PEG-HbV (Hb; 5.8 mg/dl) was assessed by examining an agonist-induced aggregation response, and that of relatively high concentrations of PEG-HbV (Hb; 0.29, 1 and 2 g/dl) by measuring the release of RANTES (Regulated upon activation, normal T-cell expressed and presumably secreted) from platelets, which is regarded as a marker of platelet activation. The preincubation of platelets with PEG-HbV at 5.8 mg/dl of Hb did not affect platelet aggregation induced by collagen, thrombin and ristocetin. The pretreatment of platelet-rich plasma (PRP) with PEG-HbV at concentrations up to 2 g/dl of Hb had no aberrant effects on the collagen-induced RANTES release. Furthermore, the collagen-induced release of RANTES from PRP was not affected by longer incubation with PEG-HbV at 2 g/dl of Hb. The basal levels of RANTES from PRP were unchanged in the presence of PEG-HbV. These results suggest that PEG-HbV, at the concentrations studied, have no aberrant effects on platelet functions in the presence of plasma.

Original languageEnglish
Pages (from-to)191-201
Number of pages11
JournalArtificial Cells, Blood Substitutes, and Immobilization Biotechnology
Volume29
Issue number3
DOIs
Publication statusPublished - 2001 May 22

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering

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