Effects of Resveratrol and SIRT1 on PGC-1α Activity and Mitochondrial Biogenesis

A Reevaluation

Kazuhiko Higashida, Sang Hyun Kim, Su Ryun Jung, Meiko Asaka, John O. Holloszy, Dong Ho Han

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

It has been reported that feeding mice resveratrol activates AMPK and SIRT1 in skeletal muscle leading to deacetylation and activation of PGC-1α, increased mitochondrial biogenesis, and improved running endurance. This study was done to further evaluate the effects of resveratrol, SIRT1, and PGC-1α deacetylation on mitochondrial biogenesis in muscle. Feeding rats or mice a diet containing 4 g resveratrol/kg diet had no effect on mitochondrial protein levels in muscle. High concentrations of resveratrol lowered ATP concentration and activated AMPK in C2C12 myotubes, resulting in an increase in mitochondrial proteins. Knockdown of SIRT1, or suppression of SIRT1 activity with a dominant-negative (DN) SIRT1 construct, increased PGC-1α acetylation, PGC-1α coactivator activity, and mitochondrial proteins in C2C12 cells. Expression of a DN SIRT1 in rat triceps muscle also induced an increase in mitochondrial proteins. Overexpression of SIRT1 decreased PGC-1α acetylation, PGC-1α coactivator activity, and mitochondrial proteins in C2C12 myotubes. Overexpression of SIRT1 also resulted in a decrease in mitochondrial proteins in rat triceps muscle. We conclude that, contrary to some previous reports, the mechanism by which SIRT1 regulates mitochondrial biogenesis is by inhibiting PGC-1α coactivator activity, resulting in a decrease in mitochondria. We also conclude that feeding rodents resveratrol has no effect on mitochondrial biogenesis in muscle.

Original languageEnglish
Article numbere1001603
JournalPLoS Biology
Volume11
Issue number7
DOIs
Publication statusPublished - 2013 Jul

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Mitochondrial Proteins
resveratrol
Organelle Biogenesis
Muscle
Muscles
muscles
Rats
Acetylation
AMP-Activated Protein Kinases
proteins
Skeletal Muscle Fibers
acetylation
Nutrition
rats
Diet
Mitochondria
mice
diet
skeletal muscle
biogenesis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Higashida, K., Kim, S. H., Jung, S. R., Asaka, M., Holloszy, J. O., & Han, D. H. (2013). Effects of Resveratrol and SIRT1 on PGC-1α Activity and Mitochondrial Biogenesis: A Reevaluation. PLoS Biology, 11(7), [e1001603]. https://doi.org/10.1371/journal.pbio.1001603

Effects of Resveratrol and SIRT1 on PGC-1α Activity and Mitochondrial Biogenesis : A Reevaluation. / Higashida, Kazuhiko; Kim, Sang Hyun; Jung, Su Ryun; Asaka, Meiko; Holloszy, John O.; Han, Dong Ho.

In: PLoS Biology, Vol. 11, No. 7, e1001603, 07.2013.

Research output: Contribution to journalArticle

Higashida, K, Kim, SH, Jung, SR, Asaka, M, Holloszy, JO & Han, DH 2013, 'Effects of Resveratrol and SIRT1 on PGC-1α Activity and Mitochondrial Biogenesis: A Reevaluation', PLoS Biology, vol. 11, no. 7, e1001603. https://doi.org/10.1371/journal.pbio.1001603
Higashida, Kazuhiko ; Kim, Sang Hyun ; Jung, Su Ryun ; Asaka, Meiko ; Holloszy, John O. ; Han, Dong Ho. / Effects of Resveratrol and SIRT1 on PGC-1α Activity and Mitochondrial Biogenesis : A Reevaluation. In: PLoS Biology. 2013 ; Vol. 11, No. 7.
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