Effects of sugars and polyols on basidiocarp formation in a phosphoglucose isomerase mutant of Coprinus cinereus

Hiroshi Nyunoya, T. Takemaru, T. Ishikawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Several biochemical properties of a mutant deficient in phosphoglucose isomerase (pgi) of Coprinus cinereus were examined in connection with its ability to produce basidiocarps. Mycelium of the pgi mutant accumulated glucose-6-phosphate and showed higher glucose-6-phosphate dehydrogenase activity than wild type. A conventional fruiting medium did not support basidiocarp formation by the homozygous dikaryon pgi/pgi, but the addition of a reduced amount of glucose plus a compensatory amount of fructose to the culture medium resulted in partial recovery of fruiting ability. This modification of the culture medium decreased the intracellular glucose-6-phosphate to almost the same level as in the wild type. The addition of polyols such as mannitol resulted in complete recovery of fruiting ability by the dikaryon pgi/pgi without affecting the level of glucose-6-phosphate. Mycelium of the mutant showed an elevated activity of NAD-linked polyol dehydrogenase and an elevated intracellular NAD level, irrespective of whether the mycelium was grown in the presence or absence of polyol.

Original languageEnglish
Pages (from-to)45-51
Number of pages7
JournalCanadian Journal of Microbiology
Volume30
Issue number1
Publication statusPublished - 1984
Externally publishedYes

Fingerprint

Fungal Fruiting Bodies
Coprinus
Glucose-6-Phosphate Isomerase
Glucose-6-Phosphate
Mycelium
NAD
Culture Media
L-Iditol 2-Dehydrogenase
Glucosephosphate Dehydrogenase
Mannitol
Fructose
polyol
Glucose

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Genetics

Cite this

Effects of sugars and polyols on basidiocarp formation in a phosphoglucose isomerase mutant of Coprinus cinereus. / Nyunoya, Hiroshi; Takemaru, T.; Ishikawa, T.

In: Canadian Journal of Microbiology, Vol. 30, No. 1, 1984, p. 45-51.

Research output: Contribution to journalArticle

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