Effects of titanium dioxide nanoparticle aggregate size on gene expression

Junko Okuda-Shimazaki, Saiko Takaku, Koki Kanehira, Shuji Sonezaki, Akiyohshi Taniguchi

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)

Abstract

Titanium dioxide (titania) nanoparticle aggregation is an important factor in understanding cytotoxicity. However, the effect of the aggregate size of nanoparticles on cells is unclear. We prepared two sizes of titania aggregate particles and investigated their biological activity by analyzing biomarker expression based on mRNA expression analysis. The aggregate particle sizes of small and large aggregated titania were 166 nm (PDI = 0.291) and 596 nm (PDI = 0.417), respectively. These two size groups were separated by centrifugation from the same initial nanoparticle sample. We analyzed the gene expression of biomarkers focused on stress, inflammation, and cytotoxicity. Large titania aggregates show a larger effect on cell viability and gene expression when compared with the small aggregates. This suggests that particle aggregate size is related to cellular effects.

Original languageEnglish
Pages (from-to)2383-2392
Number of pages10
JournalInternational journal of molecular sciences
Volume11
Issue number6
DOIs
Publication statusPublished - 2010 Jun
Externally publishedYes

Keywords

  • Cytotoxicity
  • Gene expression
  • Nanoparticles
  • Titanium dioxide

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Fingerprint Dive into the research topics of 'Effects of titanium dioxide nanoparticle aggregate size on gene expression'. Together they form a unique fingerprint.

Cite this