Efferent and afferent connections of the ventromedial hypothalamic nucleus determined by neural tracer analysis: Implications for lordosis regulation in female rats

Yuji Shimogawa, Yasuo Sakuma, Korehito Yamanouchi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Neural connections of the ventromedial hypothalamic nucleus (VMN) to and from forebrain and midbrain structures, which are involved in the neuroendocrine regulation of reproduction, were investigated. A retrograde (fluoro-gold [FG]) or an anterograde neural tracer (phaseolus vulgaris-leucoagglutinin [PHA-L]) was injected into the left side of the VMN in ovariectomized rats. Six days after injection with FG or 11 days after injection with PHA-L, brains were fixed and sectioned. After immunohistochemistry, digital images of FG-labeled neural cell bodies (FG-cells) or PHA-L-labeled fibers (PHA-L-fibers) were analyzed. Injection sites of FG and PHA-L were mainly in the ventrolateral VMN. Considerable numbers of FG-cells and PHA-L-fibers were present in the left side of the medial amygdala, ventral lateral septum, preoptic area, bed nucleus of stria terminalis, dorsomedial hypothalamic nucleus, arcuate nucleus, periventricular nucleus of thalamus, and midbrain central gray. The lateral dorsal raphe nuclei contained many PHA-L-fibers but few FG-cells. By contrast, both sides of the median raphe nucleus contained many FG-cells but few PHA-L-fibers. Reciprocal direct neural connection between the right and left side of the VMN were observed. The present results provide an anatomical basis for functional relationships between the VMN and these nuclei.

Original languageEnglish
Pages (from-to)19-33
Number of pages15
JournalNeuroscience Research
Volume91
DOIs
Publication statusPublished - 2015 Feb 1

Fingerprint

Ventromedial Hypothalamic Nucleus
Lordosis
Injections
Dorsomedial Hypothalamic Nucleus
Midline Thalamic Nuclei
Lateral Thalamic Nuclei
Septal Nuclei
Periaqueductal Gray
Arcuate Nucleus of Hypothalamus
Preoptic Area
Raphe Nuclei
methanesulfonate salt 2-hydroxy-4,4'-diamidinostilbene
Prosencephalon
Mesencephalon
plants leukoagglutinins
Amygdala
Reproduction
Immunohistochemistry

Keywords

  • Anterograde neural tracer
  • Female rat
  • Lordosis
  • Neural connection
  • Retrograde neural tracer
  • Ventromedial hypothalamic nucleus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Efferent and afferent connections of the ventromedial hypothalamic nucleus determined by neural tracer analysis : Implications for lordosis regulation in female rats. / Shimogawa, Yuji; Sakuma, Yasuo; Yamanouchi, Korehito.

In: Neuroscience Research, Vol. 91, 01.02.2015, p. 19-33.

Research output: Contribution to journalArticle

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