Efferent projection from the preoptic area for the control of non-shivering thermogenesis in rats

Xiao Ming Chen, Takayoshi Hosono, Tamae Yoda, Yutaka Fukuda, Kazuyuki Kanosue

Research output: Contribution to journalArticle

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Abstract

1. To investigate the characteristics of efferent projections from the preoptic area for the control of non-shivering thermogenesis, we tested the effects of thermal or chemical stimulation, and transections of the preoptic area on the activity of interscapular brown adipose tissue in cold-acclimated and non-acclimated anaesthetized rats. 2. Electrical stimulation of the ventromedial hypothalamic nucleus (VMH) elicited nonshivering thermogenesis in the brown adipose tissue (BAT); warming the preoptic area to 41.5°C completely suppressed the thermogenic response. 3. Injections of D,L-homocysteic acid (DLH; 0.5 mM, 0.3 μl) into the preoptic area also significantly attenuated BAT thermogenesis, whereas injections of control vehicle had no effect. 4. Transections of the whole hypothalamus in the coronal plane at the level of the paraventricular nucleus induced rapid and large rises in BAT and rectal temperatures. This response was not blocked by pretreatment with indomethacin. The high rectal and BAT temperatures were sustained more than 1 h, till the end of the experiment. Bilateral knife cuts that included the medial forebrain bundle but not the paraventricular nuclei elicited similar rises in BAT and rectal temperatures. Medial knife cuts had no effect. 5. These results suggest that warm-sensitive neurones in the preoptic area contribute a larger efferent signal for non-shivering thermogenesis than do cold-sensitive neurones, and that the preoptic area contributes a tonic inhibitory; input to loci involved with non-shivering thermogenesis. This efferent inhibitory signal passes via lateral, but not medial, hypothalamic pathways.

Original languageEnglish
Pages (from-to)883-892
Number of pages10
JournalJournal of Physiology
Volume512
Issue number3
DOIs
Publication statusPublished - 1998 Nov 1
Externally publishedYes

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Preoptic Area
Brown Adipose Tissue
Thermogenesis
Paraventricular Hypothalamic Nucleus
Temperature
Ventromedial Hypothalamic Nucleus
Chemical Stimulation
Medial Forebrain Bundle
Neurons
Injections
Indomethacin
Electric Stimulation
Hypothalamus
Hot Temperature

ASJC Scopus subject areas

  • Physiology

Cite this

Efferent projection from the preoptic area for the control of non-shivering thermogenesis in rats. / Chen, Xiao Ming; Hosono, Takayoshi; Yoda, Tamae; Fukuda, Yutaka; Kanosue, Kazuyuki.

In: Journal of Physiology, Vol. 512, No. 3, 01.11.1998, p. 883-892.

Research output: Contribution to journalArticle

Chen, Xiao Ming ; Hosono, Takayoshi ; Yoda, Tamae ; Fukuda, Yutaka ; Kanosue, Kazuyuki. / Efferent projection from the preoptic area for the control of non-shivering thermogenesis in rats. In: Journal of Physiology. 1998 ; Vol. 512, No. 3. pp. 883-892.
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