Efficient and stable transformation of Lactuca sativa L. cv. Cisco (lettuce) plastids

Hirosuke Kanamoto, Atsushi Yamashita, Hiroshi Asao, Satoru Okumura, Hisabumi Takase, Masahira Hattori, Akiho Yokota, Ken Ichi Tomizawa

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Transgenic plastids offer unique advantages in plant biotechnology, including high-level foreign protein expression. However, broad application of plastid genome engineering in biotechnology has been largely hampered by the lack of plastid transformation systems for major crops. Here we describe the development of a plastid transformation system for lettuce, Lactuca sativa L. cv. Cisco. The transforming DNA carries a spectinomycin-resistance gene (aadA) under the control of lettuce chloroplast regulatory expression elements, flanked by two adjacent lettuce plastid genome sequences allowing its targeted insertion between the rbcL and accD genes. On average, we obtained 1 transplastomic lettuce plant per bombardment. We show that lettuce leaf chloroplasts can express transgene-encoded GFP to ∼36% of the total soluble protein. All transplastomic T0 plants were fertile and the T1 progeny uniformly showed stability of the transgene in the chloroplast genome. This system will open up new possibilities for the efficient production of edible vaccines, pharmaceuticals, and antibodies in plants.

Original languageEnglish
Pages (from-to)205-217
Number of pages13
JournalTransgenic Research
Volume15
Issue number2
DOIs
Publication statusPublished - 2006 Apr
Externally publishedYes

Fingerprint

Lettuce
Plastids
Lactuca sativa
lettuce
plastids
Plastid Genomes
biotechnology
transgenes
chloroplasts
edible vaccines
Chloroplasts
Biotechnology
Transgenes
spectinomycin
leaf lettuce
Edible Vaccines
Chloroplast Genome
Spectinomycin
engineering
genes

Keywords

  • Lettuce
  • Plastid genome
  • Plastid transformation

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Biotechnology
  • Food Science

Cite this

Kanamoto, H., Yamashita, A., Asao, H., Okumura, S., Takase, H., Hattori, M., ... Tomizawa, K. I. (2006). Efficient and stable transformation of Lactuca sativa L. cv. Cisco (lettuce) plastids. Transgenic Research, 15(2), 205-217. https://doi.org/10.1007/s11248-005-3997-2

Efficient and stable transformation of Lactuca sativa L. cv. Cisco (lettuce) plastids. / Kanamoto, Hirosuke; Yamashita, Atsushi; Asao, Hiroshi; Okumura, Satoru; Takase, Hisabumi; Hattori, Masahira; Yokota, Akiho; Tomizawa, Ken Ichi.

In: Transgenic Research, Vol. 15, No. 2, 04.2006, p. 205-217.

Research output: Contribution to journalArticle

Kanamoto, H, Yamashita, A, Asao, H, Okumura, S, Takase, H, Hattori, M, Yokota, A & Tomizawa, KI 2006, 'Efficient and stable transformation of Lactuca sativa L. cv. Cisco (lettuce) plastids', Transgenic Research, vol. 15, no. 2, pp. 205-217. https://doi.org/10.1007/s11248-005-3997-2
Kanamoto, Hirosuke ; Yamashita, Atsushi ; Asao, Hiroshi ; Okumura, Satoru ; Takase, Hisabumi ; Hattori, Masahira ; Yokota, Akiho ; Tomizawa, Ken Ichi. / Efficient and stable transformation of Lactuca sativa L. cv. Cisco (lettuce) plastids. In: Transgenic Research. 2006 ; Vol. 15, No. 2. pp. 205-217.
@article{00885b54461041baadb6b8415c4b82a2,
title = "Efficient and stable transformation of Lactuca sativa L. cv. Cisco (lettuce) plastids",
abstract = "Transgenic plastids offer unique advantages in plant biotechnology, including high-level foreign protein expression. However, broad application of plastid genome engineering in biotechnology has been largely hampered by the lack of plastid transformation systems for major crops. Here we describe the development of a plastid transformation system for lettuce, Lactuca sativa L. cv. Cisco. The transforming DNA carries a spectinomycin-resistance gene (aadA) under the control of lettuce chloroplast regulatory expression elements, flanked by two adjacent lettuce plastid genome sequences allowing its targeted insertion between the rbcL and accD genes. On average, we obtained 1 transplastomic lettuce plant per bombardment. We show that lettuce leaf chloroplasts can express transgene-encoded GFP to ∼36{\%} of the total soluble protein. All transplastomic T0 plants were fertile and the T1 progeny uniformly showed stability of the transgene in the chloroplast genome. This system will open up new possibilities for the efficient production of edible vaccines, pharmaceuticals, and antibodies in plants.",
keywords = "Lettuce, Plastid genome, Plastid transformation",
author = "Hirosuke Kanamoto and Atsushi Yamashita and Hiroshi Asao and Satoru Okumura and Hisabumi Takase and Masahira Hattori and Akiho Yokota and Tomizawa, {Ken Ichi}",
year = "2006",
month = "4",
doi = "10.1007/s11248-005-3997-2",
language = "English",
volume = "15",
pages = "205--217",
journal = "Transgenic Research",
issn = "0962-8819",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - Efficient and stable transformation of Lactuca sativa L. cv. Cisco (lettuce) plastids

AU - Kanamoto, Hirosuke

AU - Yamashita, Atsushi

AU - Asao, Hiroshi

AU - Okumura, Satoru

AU - Takase, Hisabumi

AU - Hattori, Masahira

AU - Yokota, Akiho

AU - Tomizawa, Ken Ichi

PY - 2006/4

Y1 - 2006/4

N2 - Transgenic plastids offer unique advantages in plant biotechnology, including high-level foreign protein expression. However, broad application of plastid genome engineering in biotechnology has been largely hampered by the lack of plastid transformation systems for major crops. Here we describe the development of a plastid transformation system for lettuce, Lactuca sativa L. cv. Cisco. The transforming DNA carries a spectinomycin-resistance gene (aadA) under the control of lettuce chloroplast regulatory expression elements, flanked by two adjacent lettuce plastid genome sequences allowing its targeted insertion between the rbcL and accD genes. On average, we obtained 1 transplastomic lettuce plant per bombardment. We show that lettuce leaf chloroplasts can express transgene-encoded GFP to ∼36% of the total soluble protein. All transplastomic T0 plants were fertile and the T1 progeny uniformly showed stability of the transgene in the chloroplast genome. This system will open up new possibilities for the efficient production of edible vaccines, pharmaceuticals, and antibodies in plants.

AB - Transgenic plastids offer unique advantages in plant biotechnology, including high-level foreign protein expression. However, broad application of plastid genome engineering in biotechnology has been largely hampered by the lack of plastid transformation systems for major crops. Here we describe the development of a plastid transformation system for lettuce, Lactuca sativa L. cv. Cisco. The transforming DNA carries a spectinomycin-resistance gene (aadA) under the control of lettuce chloroplast regulatory expression elements, flanked by two adjacent lettuce plastid genome sequences allowing its targeted insertion between the rbcL and accD genes. On average, we obtained 1 transplastomic lettuce plant per bombardment. We show that lettuce leaf chloroplasts can express transgene-encoded GFP to ∼36% of the total soluble protein. All transplastomic T0 plants were fertile and the T1 progeny uniformly showed stability of the transgene in the chloroplast genome. This system will open up new possibilities for the efficient production of edible vaccines, pharmaceuticals, and antibodies in plants.

KW - Lettuce

KW - Plastid genome

KW - Plastid transformation

UR - http://www.scopus.com/inward/record.url?scp=33645679058&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645679058&partnerID=8YFLogxK

U2 - 10.1007/s11248-005-3997-2

DO - 10.1007/s11248-005-3997-2

M3 - Article

VL - 15

SP - 205

EP - 217

JO - Transgenic Research

JF - Transgenic Research

SN - 0962-8819

IS - 2

ER -