Efficient depletion of fission yeast condensin by combined transcriptional repression and auxin-induced degradation

Yasutaka Kakui, Frank Uhlmann

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Structural maintenance of chromosomes (SMC) complexes play pivotal roles in controlling chromatin organization. Condensin is an essential SMC complex that compacts chromatin to form condensed chromosomes in mitosis. Complete condensin inactivation is necessary to reveal how condensin converts interphase chromatin into mitotic chromosomes. Here, we have developed a condensin depletion system in fission yeast that combines transcriptional repression with auxin-inducible protein degradation. This achieves efficient condensin depletion without need for a temperature shift. Our system is useful when studying how condensin contributes to chromosome architecture and is applicable to the study of other SMC complexes.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages25-33
Number of pages9
DOIs
Publication statusPublished - 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2004
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Auxin-inducible degron
  • Chromosome condensation
  • Condensin
  • Fission yeast
  • SMC complex
  • Transcriptional repression

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Kakui, Y., & Uhlmann, F. (2019). Efficient depletion of fission yeast condensin by combined transcriptional repression and auxin-induced degradation. In Methods in Molecular Biology (pp. 25-33). (Methods in Molecular Biology; Vol. 2004). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9520-2_3