Abstract
DNA recovery using solid-phase extraction is a fundamental technique in molecular biology and biotechnology. Our research group developed a novel DNA recovery method using amine-modified magnetic nanoparticles (MNPs) as a solid support. The use of MNPs simplifies the DNA recovery processes and permits their use in automated systems. In this study, we prepared polyamidoamine-modified superparamagnetic particles (PAMAM-SpMNPs) with 10-nm magnetite cores and used them for DNA recovery. To improve the DNA-release efficiency, the surface amine numbers on the particles were evaluated to modify each generation of PAMAM. With this optimization, the PAMAM-SpMNPs maintained a high DNA adsorption capacity and high dispersivity in solution. As a result, the DNA release from the PAMAM-SpMNPs of every generation was highly efficient. In particular, the release of DNA from the G4 to G6 PAMAM-SpMNPs was greater than 95%. Furthermore, an alternating magnetic field (AMF) was applied to expedite the DNA release from the PAMAM-SpMNPs. Complete DNA release was achieved using AMF treatment for 10 min. The DNA recovery method using the PAMAM-SpMNPs will permit various types of testing using DNA from a low volume sample, such as in a micro total analytical system.
| Original language | English |
|---|---|
| Pages (from-to) | 672-677 |
| Number of pages | 6 |
| Journal | Polymer Journal |
| Volume | 44 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2012 Jun |
| Externally published | Yes |
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Keywords
- Alternating magnetic field
- DNA extraction
- DNA release
- Magnetic nanoparticles
- PAMAM dendrimer
ASJC Scopus subject areas
- Polymers and Plastics
- Materials Chemistry
Cite this
Efficient DNA release from PAMAM dendrimer-modified superparamagnetic nanoparticles for DNA recovery. / Arakaki, Atsushi; Shibata, Keiyu; Mogi, Takeyuki; Hosokawa, Masahito; Hatakeyama, Keiichi; Gomyo, Hideyuki; Taguchi, Tomoyuki; Wake, Hitoshi; Tanaami, Takeo; Matsunaga, Tadashi; Tanaka, Tsuyoshi.
In: Polymer Journal, Vol. 44, No. 6, 06.2012, p. 672-677.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Efficient DNA release from PAMAM dendrimer-modified superparamagnetic nanoparticles for DNA recovery
AU - Arakaki, Atsushi
AU - Shibata, Keiyu
AU - Mogi, Takeyuki
AU - Hosokawa, Masahito
AU - Hatakeyama, Keiichi
AU - Gomyo, Hideyuki
AU - Taguchi, Tomoyuki
AU - Wake, Hitoshi
AU - Tanaami, Takeo
AU - Matsunaga, Tadashi
AU - Tanaka, Tsuyoshi
PY - 2012/6
Y1 - 2012/6
N2 - DNA recovery using solid-phase extraction is a fundamental technique in molecular biology and biotechnology. Our research group developed a novel DNA recovery method using amine-modified magnetic nanoparticles (MNPs) as a solid support. The use of MNPs simplifies the DNA recovery processes and permits their use in automated systems. In this study, we prepared polyamidoamine-modified superparamagnetic particles (PAMAM-SpMNPs) with 10-nm magnetite cores and used them for DNA recovery. To improve the DNA-release efficiency, the surface amine numbers on the particles were evaluated to modify each generation of PAMAM. With this optimization, the PAMAM-SpMNPs maintained a high DNA adsorption capacity and high dispersivity in solution. As a result, the DNA release from the PAMAM-SpMNPs of every generation was highly efficient. In particular, the release of DNA from the G4 to G6 PAMAM-SpMNPs was greater than 95%. Furthermore, an alternating magnetic field (AMF) was applied to expedite the DNA release from the PAMAM-SpMNPs. Complete DNA release was achieved using AMF treatment for 10 min. The DNA recovery method using the PAMAM-SpMNPs will permit various types of testing using DNA from a low volume sample, such as in a micro total analytical system.
AB - DNA recovery using solid-phase extraction is a fundamental technique in molecular biology and biotechnology. Our research group developed a novel DNA recovery method using amine-modified magnetic nanoparticles (MNPs) as a solid support. The use of MNPs simplifies the DNA recovery processes and permits their use in automated systems. In this study, we prepared polyamidoamine-modified superparamagnetic particles (PAMAM-SpMNPs) with 10-nm magnetite cores and used them for DNA recovery. To improve the DNA-release efficiency, the surface amine numbers on the particles were evaluated to modify each generation of PAMAM. With this optimization, the PAMAM-SpMNPs maintained a high DNA adsorption capacity and high dispersivity in solution. As a result, the DNA release from the PAMAM-SpMNPs of every generation was highly efficient. In particular, the release of DNA from the G4 to G6 PAMAM-SpMNPs was greater than 95%. Furthermore, an alternating magnetic field (AMF) was applied to expedite the DNA release from the PAMAM-SpMNPs. Complete DNA release was achieved using AMF treatment for 10 min. The DNA recovery method using the PAMAM-SpMNPs will permit various types of testing using DNA from a low volume sample, such as in a micro total analytical system.
KW - Alternating magnetic field
KW - DNA extraction
KW - DNA release
KW - Magnetic nanoparticles
KW - PAMAM dendrimer
UR - http://www.scopus.com/inward/record.url?scp=84861942600&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84861942600&partnerID=8YFLogxK
U2 - 10.1038/pj.2012.32
DO - 10.1038/pj.2012.32
M3 - Article
AN - SCOPUS:84861942600
VL - 44
SP - 672
EP - 677
JO - Polymer Journal
JF - Polymer Journal
SN - 0032-3896
IS - 6
ER -