Efficient DNA release from PAMAM dendrimer-modified superparamagnetic nanoparticles for DNA recovery

Atsushi Arakaki, Keiyu Shibata, Takeyuki Mogi, Masahito Hosokawa, Keiichi Hatakeyama, Hideyuki Gomyo, Tomoyuki Taguchi, Hitoshi Wake, Takeo Tanaami, Tadashi Matsunaga, Tsuyoshi Tanaka

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

DNA recovery using solid-phase extraction is a fundamental technique in molecular biology and biotechnology. Our research group developed a novel DNA recovery method using amine-modified magnetic nanoparticles (MNPs) as a solid support. The use of MNPs simplifies the DNA recovery processes and permits their use in automated systems. In this study, we prepared polyamidoamine-modified superparamagnetic particles (PAMAM-SpMNPs) with 10-nm magnetite cores and used them for DNA recovery. To improve the DNA-release efficiency, the surface amine numbers on the particles were evaluated to modify each generation of PAMAM. With this optimization, the PAMAM-SpMNPs maintained a high DNA adsorption capacity and high dispersivity in solution. As a result, the DNA release from the PAMAM-SpMNPs of every generation was highly efficient. In particular, the release of DNA from the G4 to G6 PAMAM-SpMNPs was greater than 95%. Furthermore, an alternating magnetic field (AMF) was applied to expedite the DNA release from the PAMAM-SpMNPs. Complete DNA release was achieved using AMF treatment for 10 min. The DNA recovery method using the PAMAM-SpMNPs will permit various types of testing using DNA from a low volume sample, such as in a micro total analytical system.

Original languageEnglish
Pages (from-to)672-677
Number of pages6
JournalPolymer Journal
Volume44
Issue number6
DOIs
Publication statusPublished - 2012 Jun
Externally publishedYes

Fingerprint

Dendrimers
DNA
Nanoparticles
Recovery
Amines
PAMAM Starburst
Ferrosoferric Oxide
Magnetic fields
Molecular biology
Magnetite
Biotechnology
Poly(amidoamine)

Keywords

  • Alternating magnetic field
  • DNA extraction
  • DNA release
  • Magnetic nanoparticles
  • PAMAM dendrimer

ASJC Scopus subject areas

  • Polymers and Plastics
  • Materials Chemistry

Cite this

Efficient DNA release from PAMAM dendrimer-modified superparamagnetic nanoparticles for DNA recovery. / Arakaki, Atsushi; Shibata, Keiyu; Mogi, Takeyuki; Hosokawa, Masahito; Hatakeyama, Keiichi; Gomyo, Hideyuki; Taguchi, Tomoyuki; Wake, Hitoshi; Tanaami, Takeo; Matsunaga, Tadashi; Tanaka, Tsuyoshi.

In: Polymer Journal, Vol. 44, No. 6, 06.2012, p. 672-677.

Research output: Contribution to journalArticle

Arakaki, A, Shibata, K, Mogi, T, Hosokawa, M, Hatakeyama, K, Gomyo, H, Taguchi, T, Wake, H, Tanaami, T, Matsunaga, T & Tanaka, T 2012, 'Efficient DNA release from PAMAM dendrimer-modified superparamagnetic nanoparticles for DNA recovery', Polymer Journal, vol. 44, no. 6, pp. 672-677. https://doi.org/10.1038/pj.2012.32
Arakaki, Atsushi ; Shibata, Keiyu ; Mogi, Takeyuki ; Hosokawa, Masahito ; Hatakeyama, Keiichi ; Gomyo, Hideyuki ; Taguchi, Tomoyuki ; Wake, Hitoshi ; Tanaami, Takeo ; Matsunaga, Tadashi ; Tanaka, Tsuyoshi. / Efficient DNA release from PAMAM dendrimer-modified superparamagnetic nanoparticles for DNA recovery. In: Polymer Journal. 2012 ; Vol. 44, No. 6. pp. 672-677.
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AB - DNA recovery using solid-phase extraction is a fundamental technique in molecular biology and biotechnology. Our research group developed a novel DNA recovery method using amine-modified magnetic nanoparticles (MNPs) as a solid support. The use of MNPs simplifies the DNA recovery processes and permits their use in automated systems. In this study, we prepared polyamidoamine-modified superparamagnetic particles (PAMAM-SpMNPs) with 10-nm magnetite cores and used them for DNA recovery. To improve the DNA-release efficiency, the surface amine numbers on the particles were evaluated to modify each generation of PAMAM. With this optimization, the PAMAM-SpMNPs maintained a high DNA adsorption capacity and high dispersivity in solution. As a result, the DNA release from the PAMAM-SpMNPs of every generation was highly efficient. In particular, the release of DNA from the G4 to G6 PAMAM-SpMNPs was greater than 95%. Furthermore, an alternating magnetic field (AMF) was applied to expedite the DNA release from the PAMAM-SpMNPs. Complete DNA release was achieved using AMF treatment for 10 min. The DNA recovery method using the PAMAM-SpMNPs will permit various types of testing using DNA from a low volume sample, such as in a micro total analytical system.

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