Efficient gene transfer of high copy number mibilizable plasmids containing a marine Rhodobacter origin of replication

J. Grant Burgess; Hiroaki Sudo; Koji Sode; Tadashi Matsunaga

doi:10.1007/BF01577344

Efficient gene transfer of high copy number mibilizable plasmids containing a marine Rhodobacter origin of replication

J. Grant Burgess, Hiroaki Sudo, Koji Sode, Tadashi Matsunaga^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The replication region of pRD31, a 3.1-kb endogenous plasmid from a marine Rhodobacter species, has recently been localized and sequenced. We report here incorporation of this replicon into a narrow-host-range mobilizable pBR325 derivative. This has allowed us to establish an efficient conjugative gene transfer system for a hydrogen-producing marine species of Rhodobacter that is unable to grow aerobically. Efficient transfer was obtained (1.2×10^-3 transconjugants per recipient cell), and hybrid plasmids replicated with a high copy number (>10) and good stability. Southern hybridization analysis indicated that the new vectors, pRDP203 and pRDP203s, were maintained in marine Rhodobacter sp. NKPB0021 as autonomous replicons without detrimental structural rearrangements, confirming their suitability for use as shuttle vectors.

Original language	English
Pages (from-to)	105-108
Number of pages	4
Journal	Current Microbiology
Volume	26
Issue number	2
DOIs	https://doi.org/10.1007/BF01577344
Publication status	Published - 1993 Feb
Externally published	Yes

ASJC Scopus subject areas

Microbiology

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/BF01577344

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abstract = "The replication region of pRD31, a 3.1-kb endogenous plasmid from a marine Rhodobacter species, has recently been localized and sequenced. We report here incorporation of this replicon into a narrow-host-range mobilizable pBR325 derivative. This has allowed us to establish an efficient conjugative gene transfer system for a hydrogen-producing marine species of Rhodobacter that is unable to grow aerobically. Efficient transfer was obtained (1.2×10-3 transconjugants per recipient cell), and hybrid plasmids replicated with a high copy number (>10) and good stability. Southern hybridization analysis indicated that the new vectors, pRDP203 and pRDP203s, were maintained in marine Rhodobacter sp. NKPB0021 as autonomous replicons without detrimental structural rearrangements, confirming their suitability for use as shuttle vectors.",

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AU - Burgess, J. Grant

AU - Sudo, Hiroaki

AU - Sode, Koji

AU - Matsunaga, Tadashi

PY - 1993/2

Y1 - 1993/2

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AB - The replication region of pRD31, a 3.1-kb endogenous plasmid from a marine Rhodobacter species, has recently been localized and sequenced. We report here incorporation of this replicon into a narrow-host-range mobilizable pBR325 derivative. This has allowed us to establish an efficient conjugative gene transfer system for a hydrogen-producing marine species of Rhodobacter that is unable to grow aerobically. Efficient transfer was obtained (1.2×10-3 transconjugants per recipient cell), and hybrid plasmids replicated with a high copy number (>10) and good stability. Southern hybridization analysis indicated that the new vectors, pRDP203 and pRDP203s, were maintained in marine Rhodobacter sp. NKPB0021 as autonomous replicons without detrimental structural rearrangements, confirming their suitability for use as shuttle vectors.

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Efficient gene transfer of high copy number mibilizable plasmids containing a marine Rhodobacter origin of replication

Abstract

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