Efficient plastid transformation in tobacco using small gold particles (0.07-0.3 μm)

Ayako Okuzaki, Shoko Kida, Junpei Watanabe, Izumi Hirasawa, Yutaka Tabei

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Plastid transformation methods have been developed for 20 plant species. However, only a few plant species, such as tobacco and lettuce, have been used in applied studies because transformation efficiencies are extremely low in other species. Plastid transformation has been mainly performed by particle bombardment using 0.6-μm gold particles as microcarriers of the transformation vector. Because the target materials in some plant species are undeveloped proplastids rather than fully developed chloroplasts, optimizing microcarrier size for the target size is a major consideration. In this study, we evaluated the availability of gold particles (0.07, 0.08, 0.1, 0.2, and 0.3 μm) that were smaller than those used for plastid transformation in previous studies. We obtained stable plastid transformants of tobacco with sufficient efficiency using all the tested small gold particles as the same level as 0.6-μm gold particles, even the smallest (0.07 μm). The average number of transformants obtained with 0.3-μm particles (9.3±4.6 per plate) was the highest among the tested gold particles. Because small gold particles were revealed to be sufficient for plastid transformation in a model tobacco plant, it is suggested that choosing appropriate small-sized gold particles which have never been used before will improve plastid transformation in many plant species.

    Original languageEnglish
    Pages (from-to)65-72
    Number of pages8
    JournalPlant Biotechnology
    Volume30
    Issue number1
    DOIs
    Publication statusPublished - 2013

    Fingerprint

    Plastids
    Gold
    gold
    plastids
    Tobacco
    tobacco
    Lettuce
    Chloroplasts
    lettuce
    chloroplasts

    Keywords

    • Chloroplasts
    • Gold particles
    • Particle bombardment
    • Plastid transformation
    • Tobacco

    ASJC Scopus subject areas

    • Plant Science
    • Biotechnology
    • Agronomy and Crop Science

    Cite this

    Efficient plastid transformation in tobacco using small gold particles (0.07-0.3 μm). / Okuzaki, Ayako; Kida, Shoko; Watanabe, Junpei; Hirasawa, Izumi; Tabei, Yutaka.

    In: Plant Biotechnology, Vol. 30, No. 1, 2013, p. 65-72.

    Research output: Contribution to journalArticle

    Okuzaki, Ayako ; Kida, Shoko ; Watanabe, Junpei ; Hirasawa, Izumi ; Tabei, Yutaka. / Efficient plastid transformation in tobacco using small gold particles (0.07-0.3 μm). In: Plant Biotechnology. 2013 ; Vol. 30, No. 1. pp. 65-72.
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