Abstract
Using the PURE (Protein synthesis Using Recombinant Elements) system, we developed an efficient and highly controllable ribosome display method for selection of functional protein. The PURE system is composed of purified factors and enzymes that are responsible for gene expression in Escherichia coli. We performed the detailed analyses and optimization of the ribosome display system and demonstrated the formation of stable mRNA/ribosome/polypeptide ternary complexes. As complex formation is fundamental to successful ribosome display, these improvements resulted in a dramatic increase in the mRNA recovery rate. As a result, a ∼12,000-fold enrichment of single-chain antibody (scFv) cDNA was achieved in a single round of selection. Specific selection of scFv mRNA from a 1:1010 dilution in competitor mRNA was achieved with only three rounds of affinity selection. These findings, together with the results in the accompanying paper [T. Matsuura, H. Yanagida, J. Ushioda, I. Urabe, T. Yomo, Nascent chain, RNA, and ribosome complexes generated by pure translation system (see the accompanying paper).], demonstrate that the PURE system can provide a basis for reliable and reproducible ribosome display.
| Original language | English |
|---|---|
| Pages (from-to) | 270-276 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 352 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2007 Jan 5 |
| Externally published | Yes |
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Keywords
- Antibody
- Cell-free protein synthesis system
- In vitro selection
- In vitro translation
- Molecular evolution
- Protein engineering
- PURE system
- Ribosome display
- scFv
- Translation
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Efficient protein selection based on ribosome display system with purified components. / Ohashi, Hiroyuki; Shimizu, Yoshihiro; Ying, Bei Wen; Ueda, Takuya.
In: Biochemical and Biophysical Research Communications, Vol. 352, No. 1, 05.01.2007, p. 270-276.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Efficient protein selection based on ribosome display system with purified components
AU - Ohashi, Hiroyuki
AU - Shimizu, Yoshihiro
AU - Ying, Bei Wen
AU - Ueda, Takuya
PY - 2007/1/5
Y1 - 2007/1/5
N2 - Using the PURE (Protein synthesis Using Recombinant Elements) system, we developed an efficient and highly controllable ribosome display method for selection of functional protein. The PURE system is composed of purified factors and enzymes that are responsible for gene expression in Escherichia coli. We performed the detailed analyses and optimization of the ribosome display system and demonstrated the formation of stable mRNA/ribosome/polypeptide ternary complexes. As complex formation is fundamental to successful ribosome display, these improvements resulted in a dramatic increase in the mRNA recovery rate. As a result, a ∼12,000-fold enrichment of single-chain antibody (scFv) cDNA was achieved in a single round of selection. Specific selection of scFv mRNA from a 1:1010 dilution in competitor mRNA was achieved with only three rounds of affinity selection. These findings, together with the results in the accompanying paper [T. Matsuura, H. Yanagida, J. Ushioda, I. Urabe, T. Yomo, Nascent chain, RNA, and ribosome complexes generated by pure translation system (see the accompanying paper).], demonstrate that the PURE system can provide a basis for reliable and reproducible ribosome display.
AB - Using the PURE (Protein synthesis Using Recombinant Elements) system, we developed an efficient and highly controllable ribosome display method for selection of functional protein. The PURE system is composed of purified factors and enzymes that are responsible for gene expression in Escherichia coli. We performed the detailed analyses and optimization of the ribosome display system and demonstrated the formation of stable mRNA/ribosome/polypeptide ternary complexes. As complex formation is fundamental to successful ribosome display, these improvements resulted in a dramatic increase in the mRNA recovery rate. As a result, a ∼12,000-fold enrichment of single-chain antibody (scFv) cDNA was achieved in a single round of selection. Specific selection of scFv mRNA from a 1:1010 dilution in competitor mRNA was achieved with only three rounds of affinity selection. These findings, together with the results in the accompanying paper [T. Matsuura, H. Yanagida, J. Ushioda, I. Urabe, T. Yomo, Nascent chain, RNA, and ribosome complexes generated by pure translation system (see the accompanying paper).], demonstrate that the PURE system can provide a basis for reliable and reproducible ribosome display.
KW - Antibody
KW - Cell-free protein synthesis system
KW - In vitro selection
KW - In vitro translation
KW - Molecular evolution
KW - Protein engineering
KW - PURE system
KW - Ribosome display
KW - scFv
KW - Translation
UR - http://www.scopus.com/inward/record.url?scp=33751410139&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33751410139&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2006.11.017
DO - 10.1016/j.bbrc.2006.11.017
M3 - Article
C2 - 17113037
AN - SCOPUS:33751410139
VL - 352
SP - 270
EP - 276
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -