Elastic scattering spectroscopy of coagulated brain tissues

Filiz Ates, Haşim Özgür Tabakoǧlu, Özgüncem Bozkulak, Murat Canpolat, Murat Gülsoy

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The goal of this study was to differentiate the parts of lamb brain according to elastic scattering spectroscopy and detect the optical alterations due to coagulation. Cells and tissues are not uniform and have complex structures and shapes. They can he referred to as scattering particles. The process of scattering depends on the light wavelength and on the scattering medium properties; especially on the size and the density of the medium. When elastic scattering spectroscopy (ESS) is employed, the morphological alterations of tissues can be detected using spectral measurements of the elastic scattered light over a wide range of wavelengths. In this study firstly, the slopes of ESS spectra were used to differentiate the parts of lamb brains (brainstem, cerebellum, gray matter, white matter) in vitro in the range of 450 - 750 nm. Secondly, tissues were coagulated at different temperatures (45, 60, and 80°C and ESS spectra were taken from native and coagulated tissues. It was observed that as the coagulation temperature increased, the slope of the elastic scattering spectra decreased. Thus, optical properties of tissues were changed with respect to the change in nuclear to cytoplasmic ratio due to the water loss. Results showed that the slopes of ESS spectra in the visible range revealed valuable information about the morphological changes caused by coagulation.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Volume6084
DOIs
Publication statusPublished - 2006
Externally publishedYes
EventOptical Interactions with Tissue and Cells XVII - San Jose, CA, United States
Duration: 2006 Jan 232006 Jan 25

Other

OtherOptical Interactions with Tissue and Cells XVII
CountryUnited States
CitySan Jose, CA
Period06/1/2306/1/25

Fingerprint

Elastic scattering
brain
Brain
elastic scattering
Spectroscopy
Tissue
coagulation
Coagulation
spectroscopy
Scattering
slopes
scattering
cerebellum
water loss
Wavelength
wavelengths
Optical properties
optical properties
Temperature
temperature

Keywords

  • Optical properties
  • Temperature
  • Visible spectra

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Ates, F., Tabakoǧlu, H. Ö., Bozkulak, Ö., Canpolat, M., & Gülsoy, M. (2006). Elastic scattering spectroscopy of coagulated brain tissues. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 6084). [60840Q] https://doi.org/10.1117/12.646195

Elastic scattering spectroscopy of coagulated brain tissues. / Ates, Filiz; Tabakoǧlu, Haşim Özgür; Bozkulak, Özgüncem; Canpolat, Murat; Gülsoy, Murat.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 6084 2006. 60840Q.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ates, F, Tabakoǧlu, HÖ, Bozkulak, Ö, Canpolat, M & Gülsoy, M 2006, Elastic scattering spectroscopy of coagulated brain tissues. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 6084, 60840Q, Optical Interactions with Tissue and Cells XVII, San Jose, CA, United States, 06/1/23. https://doi.org/10.1117/12.646195
Ates F, Tabakoǧlu HÖ, Bozkulak Ö, Canpolat M, Gülsoy M. Elastic scattering spectroscopy of coagulated brain tissues. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 6084. 2006. 60840Q https://doi.org/10.1117/12.646195
Ates, Filiz ; Tabakoǧlu, Haşim Özgür ; Bozkulak, Özgüncem ; Canpolat, Murat ; Gülsoy, Murat. / Elastic scattering spectroscopy of coagulated brain tissues. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 6084 2006.
@inproceedings{df2af3682a6b46f4bfa819180269e309,
title = "Elastic scattering spectroscopy of coagulated brain tissues",
abstract = "The goal of this study was to differentiate the parts of lamb brain according to elastic scattering spectroscopy and detect the optical alterations due to coagulation. Cells and tissues are not uniform and have complex structures and shapes. They can he referred to as scattering particles. The process of scattering depends on the light wavelength and on the scattering medium properties; especially on the size and the density of the medium. When elastic scattering spectroscopy (ESS) is employed, the morphological alterations of tissues can be detected using spectral measurements of the elastic scattered light over a wide range of wavelengths. In this study firstly, the slopes of ESS spectra were used to differentiate the parts of lamb brains (brainstem, cerebellum, gray matter, white matter) in vitro in the range of 450 - 750 nm. Secondly, tissues were coagulated at different temperatures (45, 60, and 80°C and ESS spectra were taken from native and coagulated tissues. It was observed that as the coagulation temperature increased, the slope of the elastic scattering spectra decreased. Thus, optical properties of tissues were changed with respect to the change in nuclear to cytoplasmic ratio due to the water loss. Results showed that the slopes of ESS spectra in the visible range revealed valuable information about the morphological changes caused by coagulation.",
keywords = "Optical properties, Temperature, Visible spectra",
author = "Filiz Ates and Tabakoǧlu, {Haşim {\"O}zg{\"u}r} and {\"O}zg{\"u}ncem Bozkulak and Murat Canpolat and Murat G{\"u}lsoy",
year = "2006",
doi = "10.1117/12.646195",
language = "English",
isbn = "0819461261",
volume = "6084",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

}

TY - GEN

T1 - Elastic scattering spectroscopy of coagulated brain tissues

AU - Ates, Filiz

AU - Tabakoǧlu, Haşim Özgür

AU - Bozkulak, Özgüncem

AU - Canpolat, Murat

AU - Gülsoy, Murat

PY - 2006

Y1 - 2006

N2 - The goal of this study was to differentiate the parts of lamb brain according to elastic scattering spectroscopy and detect the optical alterations due to coagulation. Cells and tissues are not uniform and have complex structures and shapes. They can he referred to as scattering particles. The process of scattering depends on the light wavelength and on the scattering medium properties; especially on the size and the density of the medium. When elastic scattering spectroscopy (ESS) is employed, the morphological alterations of tissues can be detected using spectral measurements of the elastic scattered light over a wide range of wavelengths. In this study firstly, the slopes of ESS spectra were used to differentiate the parts of lamb brains (brainstem, cerebellum, gray matter, white matter) in vitro in the range of 450 - 750 nm. Secondly, tissues were coagulated at different temperatures (45, 60, and 80°C and ESS spectra were taken from native and coagulated tissues. It was observed that as the coagulation temperature increased, the slope of the elastic scattering spectra decreased. Thus, optical properties of tissues were changed with respect to the change in nuclear to cytoplasmic ratio due to the water loss. Results showed that the slopes of ESS spectra in the visible range revealed valuable information about the morphological changes caused by coagulation.

AB - The goal of this study was to differentiate the parts of lamb brain according to elastic scattering spectroscopy and detect the optical alterations due to coagulation. Cells and tissues are not uniform and have complex structures and shapes. They can he referred to as scattering particles. The process of scattering depends on the light wavelength and on the scattering medium properties; especially on the size and the density of the medium. When elastic scattering spectroscopy (ESS) is employed, the morphological alterations of tissues can be detected using spectral measurements of the elastic scattered light over a wide range of wavelengths. In this study firstly, the slopes of ESS spectra were used to differentiate the parts of lamb brains (brainstem, cerebellum, gray matter, white matter) in vitro in the range of 450 - 750 nm. Secondly, tissues were coagulated at different temperatures (45, 60, and 80°C and ESS spectra were taken from native and coagulated tissues. It was observed that as the coagulation temperature increased, the slope of the elastic scattering spectra decreased. Thus, optical properties of tissues were changed with respect to the change in nuclear to cytoplasmic ratio due to the water loss. Results showed that the slopes of ESS spectra in the visible range revealed valuable information about the morphological changes caused by coagulation.

KW - Optical properties

KW - Temperature

KW - Visible spectra

UR - http://www.scopus.com/inward/record.url?scp=33646177454&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646177454&partnerID=8YFLogxK

U2 - 10.1117/12.646195

DO - 10.1117/12.646195

M3 - Conference contribution

SN - 0819461261

SN - 9780819461261

VL - 6084

BT - Proceedings of SPIE - The International Society for Optical Engineering

ER -