Electrically communicating three-dimensional cardiac tissue mimic fabricated by layered cultured cardiomyocyte sheets

Tatsuya Shimizu, Masayuki Yamato, Takumitsu Akutsu, Takahiko Shibata, Yuki Isoi, Akihiko Kikuchi, Mitsuo Umezu, Teruo Okano

    Research output: Contribution to journalArticle

    147 Citations (Scopus)

    Abstract

    Recent progress in stem cell biology is likely to provide implantable sources of human cardiomyocytes in the near future. This possibility has encouraged cardiac tissue engineering. To construct heart-like tissue, we have exploited the capabilities of novel cell culture surfaces grafted with a temperature-responsive polymer, poly(N-isopropylacrylamide) (PIPAAm), to produce intact viable monolayer cell sheets simply by reducing culture temperature. Cultured chick embryonic cardiomyocyte sheets detached from PIPAAm-grafted surfaces were layered into tissue-like laminate stacks using hydrophilic support and transfer membranes. The layered cell sheets rapidly adhered to each other, establishing cell-to-cell connections characteristic of heart tissue, including desmosomes and intercalated disks. Bilayer cell sheets pulsed spontaneously and synchronously, altering their characteristic pulsing frequency with applied electric stimulation transmitted across the sheets. These results demonstrate that electrically communicative three-dimensional cardiac constructs can be achieved by stacking monolayer cardiomyocyte sheets. Cardiac tissue engineering based on this technology will facilitate new in vitro heart models and may prove useful for in vivo cardiovascular tissue repair.

    Original languageEnglish
    Pages (from-to)110-117
    Number of pages8
    JournalJournal of Biomedical Materials Research
    Volume60
    Issue number1
    DOIs
    Publication statusPublished - 2002

    Fingerprint

    Tissue
    Tissue engineering
    Monolayers
    Cytology
    Stem cells
    Cell culture
    Laminates
    Polymers
    Repair
    Membranes
    Temperature
    poly-N-isopropylacrylamide

    Keywords

    • Cardiac tissue engineering
    • Cardiomyocyte
    • Cell sheet
    • Electric communication
    • Temperature-responsive culture surface

    ASJC Scopus subject areas

    • Biomedical Engineering
    • Biomaterials

    Cite this

    Electrically communicating three-dimensional cardiac tissue mimic fabricated by layered cultured cardiomyocyte sheets. / Shimizu, Tatsuya; Yamato, Masayuki; Akutsu, Takumitsu; Shibata, Takahiko; Isoi, Yuki; Kikuchi, Akihiko; Umezu, Mitsuo; Okano, Teruo.

    In: Journal of Biomedical Materials Research, Vol. 60, No. 1, 2002, p. 110-117.

    Research output: Contribution to journalArticle

    Shimizu, Tatsuya ; Yamato, Masayuki ; Akutsu, Takumitsu ; Shibata, Takahiko ; Isoi, Yuki ; Kikuchi, Akihiko ; Umezu, Mitsuo ; Okano, Teruo. / Electrically communicating three-dimensional cardiac tissue mimic fabricated by layered cultured cardiomyocyte sheets. In: Journal of Biomedical Materials Research. 2002 ; Vol. 60, No. 1. pp. 110-117.
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