Electrochemical deposition and surface-initiated RAFT polymerization

Protein and cell-resistant PPEGMEMA polymer brushes

Maria Celeste R Tria, Carlos David T Grande, Ramakrishna R. Ponnapati, Rigoberto C. Advincula

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

This paper introduces a novel and versatile method of grafting protein and cell-resistant poly(poly ethylene glycol methyl ether methacrylate) (PPEGMEMA) brushes on conducting Au surface. The process started with the electrochemical deposition and full characterization of an electro-active chain transfer agent (CTA) on the Au surface. The electrochemical behavior of the CTA was investigated by cyclic voltammetry (CV) while the deposition and stability of the CTA on the surface were confirmed by ellipsometry, contact angle, and X-ray photoelectron spectroscopy (XPS). The capability of the electrodeposited CTA to mediate surface-initiated reversible addition-fragmentation chain transfer (SI-RAFT) polymerization on both the polymethyl methacrylate (PMMA; model polymer) and PPEGMEMA brushes was demonstrated by the increase in thicknesses of the films after polymerization. Contact angles also decreased with the incorporation of the more hydrophilic brushes. Significant changes in the morphologies of the films before and after polymerization were also observed with atomic force microscopy (AFM) analyses. Furthermore, XPS results showed an increase in the O 1s peak intensity relative to C 1s after polymerizations, which confirmed the grafting of polyethyleneglycol (PEG) bearing brushes. The ability of the PPEGMEMA-modified Au surface to resist nonspecific adhesion of proteins and cells was monitored and confirmed by XPS, ellipsometry, contact angle, AFM, and fluorescence imaging. The new method presented has potential application as robust protein and cell-resistant coatings for electrically conducting electrodes and biomedical devices.

Original languageEnglish
Pages (from-to)3422-3431
Number of pages10
JournalBiomacromolecules
Volume11
Issue number12
DOIs
Publication statusPublished - 2010 Dec 13
Externally publishedYes

Fingerprint

Methyl Ethers
Methacrylates
Brushes
Polyethylene glycols
Ethers
Polymers
Polymerization
Proteins
Contact angle
X ray photoelectron spectroscopy
Ellipsometry
Polymethyl Methacrylate
Atomic force microscopy
Bearings (structural)
Polymethyl methacrylates
Cyclic voltammetry
Adhesion
Fluorescence
Imaging techniques
Coatings

ASJC Scopus subject areas

  • Bioengineering
  • Materials Chemistry
  • Polymers and Plastics
  • Biomaterials

Cite this

Electrochemical deposition and surface-initiated RAFT polymerization : Protein and cell-resistant PPEGMEMA polymer brushes. / Tria, Maria Celeste R; Grande, Carlos David T; Ponnapati, Ramakrishna R.; Advincula, Rigoberto C.

In: Biomacromolecules, Vol. 11, No. 12, 13.12.2010, p. 3422-3431.

Research output: Contribution to journalArticle

Tria, Maria Celeste R ; Grande, Carlos David T ; Ponnapati, Ramakrishna R. ; Advincula, Rigoberto C. / Electrochemical deposition and surface-initiated RAFT polymerization : Protein and cell-resistant PPEGMEMA polymer brushes. In: Biomacromolecules. 2010 ; Vol. 11, No. 12. pp. 3422-3431.
@article{c7a7b30f751c4a8d8a59f9cbe507f9e6,
title = "Electrochemical deposition and surface-initiated RAFT polymerization: Protein and cell-resistant PPEGMEMA polymer brushes",
abstract = "This paper introduces a novel and versatile method of grafting protein and cell-resistant poly(poly ethylene glycol methyl ether methacrylate) (PPEGMEMA) brushes on conducting Au surface. The process started with the electrochemical deposition and full characterization of an electro-active chain transfer agent (CTA) on the Au surface. The electrochemical behavior of the CTA was investigated by cyclic voltammetry (CV) while the deposition and stability of the CTA on the surface were confirmed by ellipsometry, contact angle, and X-ray photoelectron spectroscopy (XPS). The capability of the electrodeposited CTA to mediate surface-initiated reversible addition-fragmentation chain transfer (SI-RAFT) polymerization on both the polymethyl methacrylate (PMMA; model polymer) and PPEGMEMA brushes was demonstrated by the increase in thicknesses of the films after polymerization. Contact angles also decreased with the incorporation of the more hydrophilic brushes. Significant changes in the morphologies of the films before and after polymerization were also observed with atomic force microscopy (AFM) analyses. Furthermore, XPS results showed an increase in the O 1s peak intensity relative to C 1s after polymerizations, which confirmed the grafting of polyethyleneglycol (PEG) bearing brushes. The ability of the PPEGMEMA-modified Au surface to resist nonspecific adhesion of proteins and cells was monitored and confirmed by XPS, ellipsometry, contact angle, AFM, and fluorescence imaging. The new method presented has potential application as robust protein and cell-resistant coatings for electrically conducting electrodes and biomedical devices.",
author = "Tria, {Maria Celeste R} and Grande, {Carlos David T} and Ponnapati, {Ramakrishna R.} and Advincula, {Rigoberto C.}",
year = "2010",
month = "12",
day = "13",
doi = "10.1021/bm1009365",
language = "English",
volume = "11",
pages = "3422--3431",
journal = "Biomacromolecules",
issn = "1525-7797",
publisher = "American Chemical Society",
number = "12",

}

TY - JOUR

T1 - Electrochemical deposition and surface-initiated RAFT polymerization

T2 - Protein and cell-resistant PPEGMEMA polymer brushes

AU - Tria, Maria Celeste R

AU - Grande, Carlos David T

AU - Ponnapati, Ramakrishna R.

AU - Advincula, Rigoberto C.

PY - 2010/12/13

Y1 - 2010/12/13

N2 - This paper introduces a novel and versatile method of grafting protein and cell-resistant poly(poly ethylene glycol methyl ether methacrylate) (PPEGMEMA) brushes on conducting Au surface. The process started with the electrochemical deposition and full characterization of an electro-active chain transfer agent (CTA) on the Au surface. The electrochemical behavior of the CTA was investigated by cyclic voltammetry (CV) while the deposition and stability of the CTA on the surface were confirmed by ellipsometry, contact angle, and X-ray photoelectron spectroscopy (XPS). The capability of the electrodeposited CTA to mediate surface-initiated reversible addition-fragmentation chain transfer (SI-RAFT) polymerization on both the polymethyl methacrylate (PMMA; model polymer) and PPEGMEMA brushes was demonstrated by the increase in thicknesses of the films after polymerization. Contact angles also decreased with the incorporation of the more hydrophilic brushes. Significant changes in the morphologies of the films before and after polymerization were also observed with atomic force microscopy (AFM) analyses. Furthermore, XPS results showed an increase in the O 1s peak intensity relative to C 1s after polymerizations, which confirmed the grafting of polyethyleneglycol (PEG) bearing brushes. The ability of the PPEGMEMA-modified Au surface to resist nonspecific adhesion of proteins and cells was monitored and confirmed by XPS, ellipsometry, contact angle, AFM, and fluorescence imaging. The new method presented has potential application as robust protein and cell-resistant coatings for electrically conducting electrodes and biomedical devices.

AB - This paper introduces a novel and versatile method of grafting protein and cell-resistant poly(poly ethylene glycol methyl ether methacrylate) (PPEGMEMA) brushes on conducting Au surface. The process started with the electrochemical deposition and full characterization of an electro-active chain transfer agent (CTA) on the Au surface. The electrochemical behavior of the CTA was investigated by cyclic voltammetry (CV) while the deposition and stability of the CTA on the surface were confirmed by ellipsometry, contact angle, and X-ray photoelectron spectroscopy (XPS). The capability of the electrodeposited CTA to mediate surface-initiated reversible addition-fragmentation chain transfer (SI-RAFT) polymerization on both the polymethyl methacrylate (PMMA; model polymer) and PPEGMEMA brushes was demonstrated by the increase in thicknesses of the films after polymerization. Contact angles also decreased with the incorporation of the more hydrophilic brushes. Significant changes in the morphologies of the films before and after polymerization were also observed with atomic force microscopy (AFM) analyses. Furthermore, XPS results showed an increase in the O 1s peak intensity relative to C 1s after polymerizations, which confirmed the grafting of polyethyleneglycol (PEG) bearing brushes. The ability of the PPEGMEMA-modified Au surface to resist nonspecific adhesion of proteins and cells was monitored and confirmed by XPS, ellipsometry, contact angle, AFM, and fluorescence imaging. The new method presented has potential application as robust protein and cell-resistant coatings for electrically conducting electrodes and biomedical devices.

UR - http://www.scopus.com/inward/record.url?scp=78650277607&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650277607&partnerID=8YFLogxK

U2 - 10.1021/bm1009365

DO - 10.1021/bm1009365

M3 - Article

VL - 11

SP - 3422

EP - 3431

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 12

ER -