Electrochemical detection of allergen in small-volume whole blood using an array microelectrode

A simple method for detection of allergic reaction

Mina Okochi, Hiroko Yokouchi, Noriyuki Nakamura, Tadashi Matsunaga

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A safe, simplified, and rapid method for detection of allergen has been developed. Serotonin, a chemical mediator secreted during an allergic reaction, was used as a marker in electrochemical detection. A 20-μL drop of whole blood was used for the electrochemical detection of allergen using an array microelectrode. When cyclic voltammetry was carried out on whole blood samples containing 1 μg/mL serotonin, an anodic peak current appeared at around 350 mV versus a silver/silver chloride electrode using a Nation-coated array microelectrode. Allergen was selectively detected using whole blood samples by applying a constant potential of 350 mv after 40 min incubation with addition of allergen. The results obtained by the electrochemical detection method correlated well with the diagnosis obtained from the amount of IgE antibody.

Original languageEnglish
Pages (from-to)480-484
Number of pages5
JournalBiotechnology and Bioengineering
Volume65
Issue number4
DOIs
Publication statusPublished - 1999 Nov 20
Externally publishedYes

Fingerprint

Allergens
Microelectrodes
Blood Volume
Hypersensitivity
Blood
Serotonin
Silver
Antibodies
Immunoglobulin E
Cyclic voltammetry
Electrodes

Keywords

  • Array microelectrode
  • Biosensor
  • Electrochemical detection
  • Serotonin
  • Whole blood

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Electrochemical detection of allergen in small-volume whole blood using an array microelectrode : A simple method for detection of allergic reaction. / Okochi, Mina; Yokouchi, Hiroko; Nakamura, Noriyuki; Matsunaga, Tadashi.

In: Biotechnology and Bioengineering, Vol. 65, No. 4, 20.11.1999, p. 480-484.

Research output: Contribution to journalArticle

@article{056b1f525eb746bbb41d80e9b9aaa8c4,
title = "Electrochemical detection of allergen in small-volume whole blood using an array microelectrode: A simple method for detection of allergic reaction",
abstract = "A safe, simplified, and rapid method for detection of allergen has been developed. Serotonin, a chemical mediator secreted during an allergic reaction, was used as a marker in electrochemical detection. A 20-μL drop of whole blood was used for the electrochemical detection of allergen using an array microelectrode. When cyclic voltammetry was carried out on whole blood samples containing 1 μg/mL serotonin, an anodic peak current appeared at around 350 mV versus a silver/silver chloride electrode using a Nation-coated array microelectrode. Allergen was selectively detected using whole blood samples by applying a constant potential of 350 mv after 40 min incubation with addition of allergen. The results obtained by the electrochemical detection method correlated well with the diagnosis obtained from the amount of IgE antibody.",
keywords = "Array microelectrode, Biosensor, Electrochemical detection, Serotonin, Whole blood",
author = "Mina Okochi and Hiroko Yokouchi and Noriyuki Nakamura and Tadashi Matsunaga",
year = "1999",
month = "11",
day = "20",
doi = "10.1002/(SICI)1097-0290(19991120)65:4<480::AID-BIT13>3.0.CO;2-F",
language = "English",
volume = "65",
pages = "480--484",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "4",

}

TY - JOUR

T1 - Electrochemical detection of allergen in small-volume whole blood using an array microelectrode

T2 - A simple method for detection of allergic reaction

AU - Okochi, Mina

AU - Yokouchi, Hiroko

AU - Nakamura, Noriyuki

AU - Matsunaga, Tadashi

PY - 1999/11/20

Y1 - 1999/11/20

N2 - A safe, simplified, and rapid method for detection of allergen has been developed. Serotonin, a chemical mediator secreted during an allergic reaction, was used as a marker in electrochemical detection. A 20-μL drop of whole blood was used for the electrochemical detection of allergen using an array microelectrode. When cyclic voltammetry was carried out on whole blood samples containing 1 μg/mL serotonin, an anodic peak current appeared at around 350 mV versus a silver/silver chloride electrode using a Nation-coated array microelectrode. Allergen was selectively detected using whole blood samples by applying a constant potential of 350 mv after 40 min incubation with addition of allergen. The results obtained by the electrochemical detection method correlated well with the diagnosis obtained from the amount of IgE antibody.

AB - A safe, simplified, and rapid method for detection of allergen has been developed. Serotonin, a chemical mediator secreted during an allergic reaction, was used as a marker in electrochemical detection. A 20-μL drop of whole blood was used for the electrochemical detection of allergen using an array microelectrode. When cyclic voltammetry was carried out on whole blood samples containing 1 μg/mL serotonin, an anodic peak current appeared at around 350 mV versus a silver/silver chloride electrode using a Nation-coated array microelectrode. Allergen was selectively detected using whole blood samples by applying a constant potential of 350 mv after 40 min incubation with addition of allergen. The results obtained by the electrochemical detection method correlated well with the diagnosis obtained from the amount of IgE antibody.

KW - Array microelectrode

KW - Biosensor

KW - Electrochemical detection

KW - Serotonin

KW - Whole blood

UR - http://www.scopus.com/inward/record.url?scp=0033589833&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033589833&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-0290(19991120)65:4<480::AID-BIT13>3.0.CO;2-F

DO - 10.1002/(SICI)1097-0290(19991120)65:4<480::AID-BIT13>3.0.CO;2-F

M3 - Article

VL - 65

SP - 480

EP - 484

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 0006-3592

IS - 4

ER -