Electrochemical detection of viable bacteria in urine and antibiotic selection

Noriyuki Nakamura, Akinori Shigematsu, Tadashi Matsunaga

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

An electrode system consisting of a basal-plane pyrolytic graphite (BPG) electrode and a porous nitrocellulose membrane filter to trap bacteria was used for the detection of bacteria in urine. The peak current of a cyclic voltammogram increased with increasing initial cell concentration of Escherichia coli in urine. Urine containing from 5 × 102 to 5 × 105 cells ml-1 was measured with this system. The susceptibility of bacteria to various antibiotics was also determined from the peak current. The minimum inhibitory concentration values obtained by the electrochemical method were in good agreement with those obtained by the conventional method.

Original languageEnglish
Pages (from-to)575-580
Number of pages6
JournalBiosensors and Bioelectronics
Volume6
Issue number7
DOIs
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

Antibiotics
Bacteria
Urine
Anti-Bacterial Agents
Electrodes
Nitrocellulose
Graphite electrodes
Collodion
Graphite
Microbial Sensitivity Tests
Escherichia coli
Membranes

Keywords

  • antibiotics
  • cyclic voltammetry
  • electrochemical detection
  • urine

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

Electrochemical detection of viable bacteria in urine and antibiotic selection. / Nakamura, Noriyuki; Shigematsu, Akinori; Matsunaga, Tadashi.

In: Biosensors and Bioelectronics, Vol. 6, No. 7, 1991, p. 575-580.

Research output: Contribution to journalArticle

Nakamura, Noriyuki ; Shigematsu, Akinori ; Matsunaga, Tadashi. / Electrochemical detection of viable bacteria in urine and antibiotic selection. In: Biosensors and Bioelectronics. 1991 ; Vol. 6, No. 7. pp. 575-580.
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