Electrochemical detection of viable bacteria in urine and antibiotic selection

Noriyuki Nakamura; Akinori Shigematsu; Tadashi Matsunaga

doi:10.1016/0956-5663(91)80022-P

Electrochemical detection of viable bacteria in urine and antibiotic selection

Noriyuki Nakamura, Akinori Shigematsu, Tadashi Matsunaga^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

An electrode system consisting of a basal-plane pyrolytic graphite (BPG) electrode and a porous nitrocellulose membrane filter to trap bacteria was used for the detection of bacteria in urine. The peak current of a cyclic voltammogram increased with increasing initial cell concentration of Escherichia coli in urine. Urine containing from 5 × 10² to 5 × 10⁵ cells ml^-1 was measured with this system. The susceptibility of bacteria to various antibiotics was also determined from the peak current. The minimum inhibitory concentration values obtained by the electrochemical method were in good agreement with those obtained by the conventional method.

Original language	English
Pages (from-to)	575-580
Number of pages	6
Journal	Biosensors and Bioelectronics
Volume	6
Issue number	7
DOIs	https://doi.org/10.1016/0956-5663(91)80022-P
Publication status	Published - 1991
Externally published	Yes

Keywords

antibiotics
cyclic voltammetry
electrochemical detection
urine

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Electrochemistry

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10.1016/0956-5663(91)80022-P

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abstract = "An electrode system consisting of a basal-plane pyrolytic graphite (BPG) electrode and a porous nitrocellulose membrane filter to trap bacteria was used for the detection of bacteria in urine. The peak current of a cyclic voltammogram increased with increasing initial cell concentration of Escherichia coli in urine. Urine containing from 5 × 102 to 5 × 105 cells ml-1 was measured with this system. The susceptibility of bacteria to various antibiotics was also determined from the peak current. The minimum inhibitory concentration values obtained by the electrochemical method were in good agreement with those obtained by the conventional method.",

keywords = "antibiotics, cyclic voltammetry, electrochemical detection, urine",

author = "Noriyuki Nakamura and Akinori Shigematsu and Tadashi Matsunaga",

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doi = "10.1016/0956-5663(91)80022-P",

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T1 - Electrochemical detection of viable bacteria in urine and antibiotic selection

AU - Nakamura, Noriyuki

AU - Shigematsu, Akinori

AU - Matsunaga, Tadashi

PY - 1991

Y1 - 1991

N2 - An electrode system consisting of a basal-plane pyrolytic graphite (BPG) electrode and a porous nitrocellulose membrane filter to trap bacteria was used for the detection of bacteria in urine. The peak current of a cyclic voltammogram increased with increasing initial cell concentration of Escherichia coli in urine. Urine containing from 5 × 102 to 5 × 105 cells ml-1 was measured with this system. The susceptibility of bacteria to various antibiotics was also determined from the peak current. The minimum inhibitory concentration values obtained by the electrochemical method were in good agreement with those obtained by the conventional method.

AB - An electrode system consisting of a basal-plane pyrolytic graphite (BPG) electrode and a porous nitrocellulose membrane filter to trap bacteria was used for the detection of bacteria in urine. The peak current of a cyclic voltammogram increased with increasing initial cell concentration of Escherichia coli in urine. Urine containing from 5 × 102 to 5 × 105 cells ml-1 was measured with this system. The susceptibility of bacteria to various antibiotics was also determined from the peak current. The minimum inhibitory concentration values obtained by the electrochemical method were in good agreement with those obtained by the conventional method.

KW - antibiotics

KW - cyclic voltammetry

KW - electrochemical detection

KW - urine

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ER -

Electrochemical detection of viable bacteria in urine and antibiotic selection

Abstract

Keywords

ASJC Scopus subject areas

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