Electrochemical disinfection of fish pathogens in seawater without the production of a lethal concentration of chlorine using a flow reactor

Tsuyoshi Tanaka, Mari Shimoda, Nozomi Shionoiri, Masahito Hosokawa, Tomoyuki Taguchi, Hitoshi Wake, Tadashi Matsunaga

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

An electrochemical disinfection system employing a honeycombed platinum coated titanium electrode was developed for the disinfection of seawater. Cell suspensions (2l, 103cells/ml) of the fish pathogens, Vibrio alginolyticus, Edwardsiella tarda, Lactococcus garvieae and Vibrio anguillarum were circulated in a reactor equipped with 10 sets of these electrodes at a flow rate of 200ml/min with an applied potential of 1.0V vs. Ag/AgCl reference electrode. The circulated cells were completely disinfected after 3h of treatment, whereas free residual chlorine generated due to seawater electrolysis was below 0.1ppm. In addition, a diphenyl-1-pyrenylphosphine fluorescent assay revealed that lipid peroxidation in the cell membranes of disinfected bacteria was induced probably by reactive oxygen species generated during electrochemical treatment.

Original languageEnglish
Pages (from-to)480-484
Number of pages5
JournalJournal of Bioscience and Bioengineering
Volume116
Issue number4
DOIs
Publication statusPublished - 2013 Oct
Externally publishedYes

Fingerprint

Disinfection
Chlorine
Seawater
Pathogens
Fish
Electrodes
Fishes
Edwardsiella tarda
Vibrio alginolyticus
Lactococcus
Electrolysis
Vibrio
Cell membranes
Titanium
Platinum
Lipids
Lipid Peroxidation
Assays
Reactive Oxygen Species
Suspensions

Keywords

  • Disinfection byproducts
  • Electrochemical disinfection
  • Flow reactor
  • Lipid peroxidation
  • Marine pathogen

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Electrochemical disinfection of fish pathogens in seawater without the production of a lethal concentration of chlorine using a flow reactor. / Tanaka, Tsuyoshi; Shimoda, Mari; Shionoiri, Nozomi; Hosokawa, Masahito; Taguchi, Tomoyuki; Wake, Hitoshi; Matsunaga, Tadashi.

In: Journal of Bioscience and Bioengineering, Vol. 116, No. 4, 10.2013, p. 480-484.

Research output: Contribution to journalArticle

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