Electrochemical disinfection of fish pathogens in seawater without the production of a lethal concentration of chlorine using a flow reactor

Tsuyoshi Tanaka, Mari Shimoda, Nozomi Shionoiri, Masahito Hosokawa, Tomoyuki Taguchi, Hitoshi Wake, Tadashi Matsunaga

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

An electrochemical disinfection system employing a honeycombed platinum coated titanium electrode was developed for the disinfection of seawater. Cell suspensions (2l, 103cells/ml) of the fish pathogens, Vibrio alginolyticus, Edwardsiella tarda, Lactococcus garvieae and Vibrio anguillarum were circulated in a reactor equipped with 10 sets of these electrodes at a flow rate of 200ml/min with an applied potential of 1.0V vs. Ag/AgCl reference electrode. The circulated cells were completely disinfected after 3h of treatment, whereas free residual chlorine generated due to seawater electrolysis was below 0.1ppm. In addition, a diphenyl-1-pyrenylphosphine fluorescent assay revealed that lipid peroxidation in the cell membranes of disinfected bacteria was induced probably by reactive oxygen species generated during electrochemical treatment.

Original languageEnglish
Pages (from-to)480-484
Number of pages5
JournalJournal of Bioscience and Bioengineering
Volume116
Issue number4
DOIs
Publication statusPublished - 2013 Oct
Externally publishedYes

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Keywords

  • Disinfection byproducts
  • Electrochemical disinfection
  • Flow reactor
  • Lipid peroxidation
  • Marine pathogen

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

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