Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G

Tae Kyu Lim, Noriyuki Nakamura, Masateru Ikehata, Tadashi Matsunaga

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This paper describes a miniaturized flow immunoassay system. Ferrocene (Fc) conjugated with anti-HCG immunoglobulin G (IgG) antibody (Fc-IgG) was prepared and used as a novel analytical reagent. The system consists of the immunoreaction part, the capillary column packed with cation exchange resin, and the flow cell for electrochemical detection of Fc-IgG. The assay yielded a linear relationship between signal and HCG concentration in the range of 0-2000 mIU/mL (L = dm3). This electrochemical flow immunoassay requires minute quantities of serum and generates highly reproducible results.

Original languageEnglish
Pages (from-to)872-874
Number of pages3
JournalElectrochemistry
Volume68
Issue number11
Publication statusPublished - 2000 Nov
Externally publishedYes

Fingerprint

Antibodies
Assays
Ion exchange
Resins
Immunoglobulin G
Positive ions
Cation Exchange Resins
ferrocene

Keywords

  • Capillary Column
  • Electrochemical Flow Immunoassay
  • Ferrocene Conjugated IgG
  • HCG

ASJC Scopus subject areas

  • Electrochemistry

Cite this

Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G. / Lim, Tae Kyu; Nakamura, Noriyuki; Ikehata, Masateru; Matsunaga, Tadashi.

In: Electrochemistry, Vol. 68, No. 11, 11.2000, p. 872-874.

Research output: Contribution to journalArticle

Lim, Tae Kyu ; Nakamura, Noriyuki ; Ikehata, Masateru ; Matsunaga, Tadashi. / Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G. In: Electrochemistry. 2000 ; Vol. 68, No. 11. pp. 872-874.
@article{d88df75e16e047cb9bf75fd83b0b8b60,
title = "Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G",
abstract = "This paper describes a miniaturized flow immunoassay system. Ferrocene (Fc) conjugated with anti-HCG immunoglobulin G (IgG) antibody (Fc-IgG) was prepared and used as a novel analytical reagent. The system consists of the immunoreaction part, the capillary column packed with cation exchange resin, and the flow cell for electrochemical detection of Fc-IgG. The assay yielded a linear relationship between signal and HCG concentration in the range of 0-2000 mIU/mL (L = dm3). This electrochemical flow immunoassay requires minute quantities of serum and generates highly reproducible results.",
keywords = "Capillary Column, Electrochemical Flow Immunoassay, Ferrocene Conjugated IgG, HCG",
author = "Lim, {Tae Kyu} and Noriyuki Nakamura and Masateru Ikehata and Tadashi Matsunaga",
year = "2000",
month = "11",
language = "English",
volume = "68",
pages = "872--874",
journal = "Electrochemistry",
issn = "1344-3542",
publisher = "Electrochemical Society of Japan",
number = "11",

}

TY - JOUR

T1 - Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G

AU - Lim, Tae Kyu

AU - Nakamura, Noriyuki

AU - Ikehata, Masateru

AU - Matsunaga, Tadashi

PY - 2000/11

Y1 - 2000/11

N2 - This paper describes a miniaturized flow immunoassay system. Ferrocene (Fc) conjugated with anti-HCG immunoglobulin G (IgG) antibody (Fc-IgG) was prepared and used as a novel analytical reagent. The system consists of the immunoreaction part, the capillary column packed with cation exchange resin, and the flow cell for electrochemical detection of Fc-IgG. The assay yielded a linear relationship between signal and HCG concentration in the range of 0-2000 mIU/mL (L = dm3). This electrochemical flow immunoassay requires minute quantities of serum and generates highly reproducible results.

AB - This paper describes a miniaturized flow immunoassay system. Ferrocene (Fc) conjugated with anti-HCG immunoglobulin G (IgG) antibody (Fc-IgG) was prepared and used as a novel analytical reagent. The system consists of the immunoreaction part, the capillary column packed with cation exchange resin, and the flow cell for electrochemical detection of Fc-IgG. The assay yielded a linear relationship between signal and HCG concentration in the range of 0-2000 mIU/mL (L = dm3). This electrochemical flow immunoassay requires minute quantities of serum and generates highly reproducible results.

KW - Capillary Column

KW - Electrochemical Flow Immunoassay

KW - Ferrocene Conjugated IgG

KW - HCG

UR - http://www.scopus.com/inward/record.url?scp=0012105058&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0012105058&partnerID=8YFLogxK

M3 - Article

VL - 68

SP - 872

EP - 874

JO - Electrochemistry

JF - Electrochemistry

SN - 1344-3542

IS - 11

ER -