This paper describes a miniaturized flow immunoassay system. Ferrocene (Fc) conjugated with anti-HCG immunoglobulin G (IgG) antibody (Fc-IgG) was prepared and used as a novel analytical reagent. The system consists of the immunoreaction part, the capillary column packed with cation exchange resin, and the flow cell for electrochemical detection of Fc-IgG. The assay yielded a linear relationship between signal and HCG concentration in the range of 0-2000 mIU/mL (L = dm3). This electrochemical flow immunoassay requires minute quantities of serum and generates highly reproducible results.
|Number of pages||3|
|Publication status||Published - 2000 Nov|
- Capillary Column
- Electrochemical Flow Immunoassay
- Ferrocene Conjugated IgG
ASJC Scopus subject areas