Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G

Tae Kyu Lim, Noriyuki Nakamura, Masateru Ikehata, Tadashi Matsunaga

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This paper describes a miniaturized flow immunoassay system. Ferrocene (Fc) conjugated with anti-HCG immunoglobulin G (IgG) antibody (Fc-IgG) was prepared and used as a novel analytical reagent. The system consists of the immunoreaction part, the capillary column packed with cation exchange resin, and the flow cell for electrochemical detection of Fc-IgG. The assay yielded a linear relationship between signal and HCG concentration in the range of 0-2000 mIU/mL (L = dm3). This electrochemical flow immunoassay requires minute quantities of serum and generates highly reproducible results.

Original languageEnglish
Pages (from-to)872-874
Number of pages3
JournalElectrochemistry
Volume68
Issue number11
Publication statusPublished - 2000 Nov
Externally publishedYes

Keywords

  • Capillary Column
  • Electrochemical Flow Immunoassay
  • Ferrocene Conjugated IgG
  • HCG

ASJC Scopus subject areas

  • Electrochemistry

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  • Cite this

    Lim, T. K., Nakamura, N., Ikehata, M., & Matsunaga, T. (2000). Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G. Electrochemistry, 68(11), 872-874.