Electrochemical killing of Vibrio alginolyticus using ferrocene-modified electrode

Mina Okochi, Noriyuki Nakamura, Tadashi Matsunaga

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Electrochemical killing of the marine gram-negative bacterium, Vibrio alginolyticus, attached to a basal-plane pyrolytic graphite electrode modified with ferrocene was carried out. The survival ratio decreased to 43% when a low potential of 0.2 V versus a saturated calomel electrode was applied for 30 min and was 3% after applying 0.4 V. After the potentials were applied, the cells were stained with the fluorescent dye, propidium iodide (PI), and the change in cell membrane permeability was observed. PI-stained cell ratio almost correlated with the dead cell ratio obtained by the colony formation method when using the bare graphite electrode. In contrast, when using the ferrocene-modified electrode, PI-stained cell ratio did not correlate with the dead cell ratio and increased when potentials of above 0.8 V were applied. When using the ferrocene-modified electrode, the killing effect was not due to the change in cell membrane permeability and might be due to the electrocatalytic oxidation of intracellular substances.

Original languageEnglish
Pages (from-to)2917-2921
Number of pages5
JournalElectrochimica Acta
Volume45
Issue number18
DOIs
Publication statusPublished - 2000 Jun 9
Externally publishedYes

Fingerprint

Propidium
Graphite electrodes
Cell membranes
Electrodes
Fluorescent Dyes
Bacteria
Dyes
Oxidation
ferrocene

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Electrochemistry

Cite this

Electrochemical killing of Vibrio alginolyticus using ferrocene-modified electrode. / Okochi, Mina; Nakamura, Noriyuki; Matsunaga, Tadashi.

In: Electrochimica Acta, Vol. 45, No. 18, 09.06.2000, p. 2917-2921.

Research output: Contribution to journalArticle

Okochi, Mina ; Nakamura, Noriyuki ; Matsunaga, Tadashi. / Electrochemical killing of Vibrio alginolyticus using ferrocene-modified electrode. In: Electrochimica Acta. 2000 ; Vol. 45, No. 18. pp. 2917-2921.
@article{0da062d14aa946ef9cf6a7b7b8139022,
title = "Electrochemical killing of Vibrio alginolyticus using ferrocene-modified electrode",
abstract = "Electrochemical killing of the marine gram-negative bacterium, Vibrio alginolyticus, attached to a basal-plane pyrolytic graphite electrode modified with ferrocene was carried out. The survival ratio decreased to 43{\%} when a low potential of 0.2 V versus a saturated calomel electrode was applied for 30 min and was 3{\%} after applying 0.4 V. After the potentials were applied, the cells were stained with the fluorescent dye, propidium iodide (PI), and the change in cell membrane permeability was observed. PI-stained cell ratio almost correlated with the dead cell ratio obtained by the colony formation method when using the bare graphite electrode. In contrast, when using the ferrocene-modified electrode, PI-stained cell ratio did not correlate with the dead cell ratio and increased when potentials of above 0.8 V were applied. When using the ferrocene-modified electrode, the killing effect was not due to the change in cell membrane permeability and might be due to the electrocatalytic oxidation of intracellular substances.",
author = "Mina Okochi and Noriyuki Nakamura and Tadashi Matsunaga",
year = "2000",
month = "6",
day = "9",
doi = "10.1016/S0013-4686(00)00368-6",
language = "English",
volume = "45",
pages = "2917--2921",
journal = "Electrochimica Acta",
issn = "0013-4686",
publisher = "Elsevier Limited",
number = "18",

}

TY - JOUR

T1 - Electrochemical killing of Vibrio alginolyticus using ferrocene-modified electrode

AU - Okochi, Mina

AU - Nakamura, Noriyuki

AU - Matsunaga, Tadashi

PY - 2000/6/9

Y1 - 2000/6/9

N2 - Electrochemical killing of the marine gram-negative bacterium, Vibrio alginolyticus, attached to a basal-plane pyrolytic graphite electrode modified with ferrocene was carried out. The survival ratio decreased to 43% when a low potential of 0.2 V versus a saturated calomel electrode was applied for 30 min and was 3% after applying 0.4 V. After the potentials were applied, the cells were stained with the fluorescent dye, propidium iodide (PI), and the change in cell membrane permeability was observed. PI-stained cell ratio almost correlated with the dead cell ratio obtained by the colony formation method when using the bare graphite electrode. In contrast, when using the ferrocene-modified electrode, PI-stained cell ratio did not correlate with the dead cell ratio and increased when potentials of above 0.8 V were applied. When using the ferrocene-modified electrode, the killing effect was not due to the change in cell membrane permeability and might be due to the electrocatalytic oxidation of intracellular substances.

AB - Electrochemical killing of the marine gram-negative bacterium, Vibrio alginolyticus, attached to a basal-plane pyrolytic graphite electrode modified with ferrocene was carried out. The survival ratio decreased to 43% when a low potential of 0.2 V versus a saturated calomel electrode was applied for 30 min and was 3% after applying 0.4 V. After the potentials were applied, the cells were stained with the fluorescent dye, propidium iodide (PI), and the change in cell membrane permeability was observed. PI-stained cell ratio almost correlated with the dead cell ratio obtained by the colony formation method when using the bare graphite electrode. In contrast, when using the ferrocene-modified electrode, PI-stained cell ratio did not correlate with the dead cell ratio and increased when potentials of above 0.8 V were applied. When using the ferrocene-modified electrode, the killing effect was not due to the change in cell membrane permeability and might be due to the electrocatalytic oxidation of intracellular substances.

UR - http://www.scopus.com/inward/record.url?scp=0033691837&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033691837&partnerID=8YFLogxK

U2 - 10.1016/S0013-4686(00)00368-6

DO - 10.1016/S0013-4686(00)00368-6

M3 - Article

AN - SCOPUS:0033691837

VL - 45

SP - 2917

EP - 2921

JO - Electrochimica Acta

JF - Electrochimica Acta

SN - 0013-4686

IS - 18

ER -