Electrochemical sterilization of bacteria using a graphite electrode modified with adsorbed ferrocene

Mina Okochi, Tadashi Matsunaga

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21 Citations (Scopus)

Abstract

Electrochemical sterilization of the marine gram-negative bacterium Vibrio alginolyticus cells was carried out using basal-plane pyrolytic graphite electrode modified with adsorbed ferrocene. When cyclic voltammetry was taken in V. alginolyticus cell suspension using an electrode modified with ferrocene at a scan rate of 20 mV per s at 25 °C, an electrocatalytic oxidation occurred above 0.1 V versus saturated calomel electrode (sce). Peak current was observed around 0.3 V vs sce. The bacteria which attached to the ferrocene modified electrode were sterilized at 0.2 V vs sce in sterile seawater, whereas they were sterilized at 0.8 V vs sce when using a bare graphite electrode. This sterilization was due to the electrochemical oxidation of the intracellular substance and not to the toxicity of ferrocene. V. alginolyticus cells in sterile seawater were completely sterilized after 10 min by applying 0.2 V vs sce.

Original languageEnglish
Pages (from-to)3247-3250
Number of pages4
JournalElectrochimica Acta
Volume42
Issue number20-22
Publication statusPublished - 1997
Externally publishedYes

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Keywords

  • Electrocatalytic oxidation
  • Electrochemical sterilization
  • Electrode modified with adsorbed ferrocene
  • Prevention of biofouling
  • Sterilization at 0.2 V vs sce

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Electrochemistry

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