Electrochemical surface plasmon resonance and waveguide-enhanced glucose biosensing with N-alkylaminated polypyrrole/glucose oxidase multilayers

Akira Baba, Prasad Taranekar, Ramakrishna R. Ponnapati, Wolfgang Knoll, Rigoberto C. Advincula

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

In this work, we report an electrochemical surface plasmon resonance/waveguide (EC-SPR/waveguide) glucose biosensor that could detect enzymatic reactions in a conducting polymer/glucose oxidase (GOx) multilayer thin film. In order to achieve a controlled enzyme electrode and waveguide mode, GOx (negatively charged) was immobilized with a water-soluble, conducting N-alkylaminated polypyrrole (positively charged) using the layer-by-layer (LbL) electrostatic self-assembly technique. The electrochemical and optical signals were simultaneously obtained from the composite LbL enzyme electrode upon the addition of glucose as mediated by the electroactivity and electrochromic property of the polypyrrole layers. Signal enhancement in EC-SPR detection is obtained by monitoring the doping-dedoping events on the polypyrrole. The real-time optical signal could be distinguished between the change in the dielectric constant of the enzyme layer and other nonenzymatic reaction events such as adsorption of glucose and the change of the refractive index of the solution. This was possible by correlation of both the SPR mode and the m = 0 and 1 modes of the waveguide in an SPR/waveguide spectroscopy experiment.

Original languageEnglish
Pages (from-to)2347-2354
Number of pages8
JournalACS Applied Materials and Interfaces
Volume2
Issue number8
DOIs
Publication statusPublished - 2010 Aug 25
Externally publishedYes

Fingerprint

Glucose Oxidase
Glucose oxidase
Polypyrroles
Surface plasmon resonance
Glucose
Multilayers
Waveguides
Enzyme electrodes
Multilayer films
Conducting polymers
Biosensors
Self assembly
Electrostatics
Refractive index
Permittivity
Enzymes
Doping (additives)
Spectroscopy
Adsorption
Thin films

Keywords

  • biosensor
  • conducting polymer
  • enzyme
  • glucose
  • surface plasmon
  • waveguide

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Electrochemical surface plasmon resonance and waveguide-enhanced glucose biosensing with N-alkylaminated polypyrrole/glucose oxidase multilayers. / Baba, Akira; Taranekar, Prasad; Ponnapati, Ramakrishna R.; Knoll, Wolfgang; Advincula, Rigoberto C.

In: ACS Applied Materials and Interfaces, Vol. 2, No. 8, 25.08.2010, p. 2347-2354.

Research output: Contribution to journalArticle

Baba, Akira ; Taranekar, Prasad ; Ponnapati, Ramakrishna R. ; Knoll, Wolfgang ; Advincula, Rigoberto C. / Electrochemical surface plasmon resonance and waveguide-enhanced glucose biosensing with N-alkylaminated polypyrrole/glucose oxidase multilayers. In: ACS Applied Materials and Interfaces. 2010 ; Vol. 2, No. 8. pp. 2347-2354.
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