Electrodes combined with an agarose stamp for addressable micropatterning

Soichiro Sekine, Shinya Nakanishi, Takeo Miyake, Kuniaki Nagamine, Hirokazu Kaji, Matsuhiko Nishizawa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have combined a topographically patterned agarose microstamp with an electrode substrate to develop a novel printing device that internally contains an electrochemical system for a controlled supply of reactive ink to the stamp surface. The 10 wt % agarose gel containing 0.1 M PBS + 25 mM KBr showed suitable elasticity for forming stamps and served as the electrolytic medium for the electrochemical oxidation of Br- to generate HBrO. The electrode substrate patched with an agarose stamp having 50-μm-high bumps was used for the spatially confined detachment of heparin/polyethyleneimine precoated on glass substrates, followed by micropatterned adsorption of fibronectin. Using the microelectrode array, the addressable micropatterning of protein by the controlled delivery of HBrO to each bump was demonstrated.

Original languageEnglish
Pages (from-to)11526-11529
Number of pages4
JournalLangmuir
Volume26
Issue number13
DOIs
Publication statusPublished - 2010 Jul 6
Externally publishedYes

Fingerprint

Sepharose
Electrodes
electrodes
Substrates
heparins
Polyethyleneimine
electrochemical oxidation
Electrochemical oxidation
Microelectrodes
inks
Fibronectins
detachment
Ink
printing
Heparin
Printing
Elasticity
delivery
Gels
elastic properties

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Sekine, S., Nakanishi, S., Miyake, T., Nagamine, K., Kaji, H., & Nishizawa, M. (2010). Electrodes combined with an agarose stamp for addressable micropatterning. Langmuir, 26(13), 11526-11529. https://doi.org/10.1021/la100735e

Electrodes combined with an agarose stamp for addressable micropatterning. / Sekine, Soichiro; Nakanishi, Shinya; Miyake, Takeo; Nagamine, Kuniaki; Kaji, Hirokazu; Nishizawa, Matsuhiko.

In: Langmuir, Vol. 26, No. 13, 06.07.2010, p. 11526-11529.

Research output: Contribution to journalArticle

Sekine, S, Nakanishi, S, Miyake, T, Nagamine, K, Kaji, H & Nishizawa, M 2010, 'Electrodes combined with an agarose stamp for addressable micropatterning', Langmuir, vol. 26, no. 13, pp. 11526-11529. https://doi.org/10.1021/la100735e
Sekine S, Nakanishi S, Miyake T, Nagamine K, Kaji H, Nishizawa M. Electrodes combined with an agarose stamp for addressable micropatterning. Langmuir. 2010 Jul 6;26(13):11526-11529. https://doi.org/10.1021/la100735e
Sekine, Soichiro ; Nakanishi, Shinya ; Miyake, Takeo ; Nagamine, Kuniaki ; Kaji, Hirokazu ; Nishizawa, Matsuhiko. / Electrodes combined with an agarose stamp for addressable micropatterning. In: Langmuir. 2010 ; Vol. 26, No. 13. pp. 11526-11529.
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