Electrolyzed hydrogen water protects against ethanol-induced cytotoxicity by regulating aldehyde metabolism-associated enzymes in the hepatic cell line hepg2

Satoshi Yano, Jinyun Wang, Shigeru Kabayama, Taichi Hara

Research output: Contribution to journalArticlepeer-review

Abstract

Excessive alcohol consumption can cause multi-systemic diseases. Among them, alcoholic liver disease is the most frequent and serious disease. Electrolytic hydrogen water (EHW) is produced at the cathode during electrolysis of water and contains a large amount of molecular hydrogen and a low content of platinum nanoparticles with alkaline properties. In this study, we found that EHW inhibits ethanol-induced cytotoxicity by decreasing the intracellular acetaldehyde, a toxic substance produced by ethanol degradation, in hepatocyte cell lines HepG2. Analysis of the mechanism of action revealed that EHW inhibits the metabolism of ethanol to acetaldehyde by suppressing alcohol dehydrogenase. EHW also promotes the metabolism of acetaldehyde to acetic acid by activating aldehyde dehydrogenase, which plays to reduce aldehyde toxicity and intracellular reactive oxygen species in HepG2 cells. These functions were correlated with the concentration of molecular hydrogen in EHW, and were abolished by degassing treatment, suggesting that molecular hydrogen may contribute as a functional factor in the suppression of ethanol-induced hepatocellular damage. Furthermore, hydrogen water with high dissolved hydrogen molecule showed the same hepatocellular protective effect against ethanol as the EHW. These results suggest that EHW may be useful in the prevention of alcoholic liver disease.

Original languageEnglish
Article number801
JournalAntioxidants
Volume10
Issue number5
DOIs
Publication statusPublished - 2021 May

Keywords

  • Alcohol dehydrogenase (ADH)
  • Alcohol toxicity
  • Alcoholic liver disease (ALD)
  • Aldehyde dehydrogenase (ALDH)
  • Electrolyzed hydrogen water
  • Hydrogen
  • Reactive oxygen species (ROS)

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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