Electronic control of H+ current in a bioprotonic device with Gramicidin A and Alamethicin

Zahra Hemmatian; Scott Keene; Erik Josberger; Takeo Miyake; Carina Arboleda; Jessica Soto-Rodríguez; François Baneyx; Marco Rolandi

doi:10.1038/ncomms12981

Electronic control of H⁺ current in a bioprotonic device with Gramicidin A and Alamethicin

Zahra Hemmatian, Scott Keene, Erik Josberger, Takeo Miyake, Carina Arboleda, Jessica Soto-Rodríguez, François Baneyx, Marco Rolandi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

In biological systems, intercellular communication is mediated by membrane proteins and ion channels that regulate traffic of ions and small molecules across cell membranes. A bioelectronic device with ion channels that control ionic flow across a supported lipid bilayer (SLB) should therefore be ideal for interfacing with biological systems. Here, we demonstrate a biotic-abiotic bioprotonic device with Pd contacts that regulates proton (H⁺) flow across an SLB incorporating the ion channels Gramicidin A (gA) and Alamethicin (ALM). We model the device characteristics using the Goldman-Hodgkin-Katz (GHK) solution to the Nernst-Planck equation for transport across the membrane. We derive the permeability for an SLB integrating gA and ALM and demonstrate pH control as a function of applied voltage and membrane permeability. This work opens the door to integrating more complex H⁺ channels at the Pd contact interface to produce responsive biotic-abiotic devices with increased functionality.

Original language	English
Article number	12981
Journal	Nature communications
Volume	7
DOIs	https://doi.org/10.1038/ncomms12981
Publication status	Published - 2016 Oct 7
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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title = "Electronic control of H+ current in a bioprotonic device with Gramicidin A and Alamethicin",

abstract = "In biological systems, intercellular communication is mediated by membrane proteins and ion channels that regulate traffic of ions and small molecules across cell membranes. A bioelectronic device with ion channels that control ionic flow across a supported lipid bilayer (SLB) should therefore be ideal for interfacing with biological systems. Here, we demonstrate a biotic-abiotic bioprotonic device with Pd contacts that regulates proton (H+) flow across an SLB incorporating the ion channels Gramicidin A (gA) and Alamethicin (ALM). We model the device characteristics using the Goldman-Hodgkin-Katz (GHK) solution to the Nernst-Planck equation for transport across the membrane. We derive the permeability for an SLB integrating gA and ALM and demonstrate pH control as a function of applied voltage and membrane permeability. This work opens the door to integrating more complex H+ channels at the Pd contact interface to produce responsive biotic-abiotic devices with increased functionality.",

author = "Zahra Hemmatian and Scott Keene and Erik Josberger and Takeo Miyake and Carina Arboleda and Jessica Soto-Rodr{\'i}guez and Fran{\c c}ois Baneyx and Marco Rolandi",

note = "Funding Information: This work is supported by the U.S. Department of Energy (DOE), Office of Science, Basic Energy Sciences, under award no. <DE-SC0010441>, device integration and device measurement and the Air Force Office of Sponsored Research under award no. <FA9550-15-1-0273>, device fabrication. Part of this work was conducted at the Washington Nanofabrication/Molecular & Nanotechnology User Facility, a member of the NSF National Nanotechnology Infrastructure Network and also at the Molecular Analysis Facility, which is supported in part by funds from the University of Washington, the Molecular Engineering & Sciences Institute, the Clean Energy Institute, the National Science Foundation and the National Institutes of Health. Publisher Copyright: {\textcopyright} The Author(s) 2016.",

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AU - Hemmatian, Zahra

AU - Keene, Scott

AU - Josberger, Erik

AU - Miyake, Takeo

AU - Arboleda, Carina

AU - Soto-Rodríguez, Jessica

AU - Baneyx, François

AU - Rolandi, Marco

N1 - Funding Information: This work is supported by the U.S. Department of Energy (DOE), Office of Science, Basic Energy Sciences, under award no. <DE-SC0010441>, device integration and device measurement and the Air Force Office of Sponsored Research under award no. <FA9550-15-1-0273>, device fabrication. Part of this work was conducted at the Washington Nanofabrication/Molecular & Nanotechnology User Facility, a member of the NSF National Nanotechnology Infrastructure Network and also at the Molecular Analysis Facility, which is supported in part by funds from the University of Washington, the Molecular Engineering & Sciences Institute, the Clean Energy Institute, the National Science Foundation and the National Institutes of Health. Publisher Copyright: © The Author(s) 2016.

PY - 2016/10/7

Y1 - 2016/10/7

N2 - In biological systems, intercellular communication is mediated by membrane proteins and ion channels that regulate traffic of ions and small molecules across cell membranes. A bioelectronic device with ion channels that control ionic flow across a supported lipid bilayer (SLB) should therefore be ideal for interfacing with biological systems. Here, we demonstrate a biotic-abiotic bioprotonic device with Pd contacts that regulates proton (H+) flow across an SLB incorporating the ion channels Gramicidin A (gA) and Alamethicin (ALM). We model the device characteristics using the Goldman-Hodgkin-Katz (GHK) solution to the Nernst-Planck equation for transport across the membrane. We derive the permeability for an SLB integrating gA and ALM and demonstrate pH control as a function of applied voltage and membrane permeability. This work opens the door to integrating more complex H+ channels at the Pd contact interface to produce responsive biotic-abiotic devices with increased functionality.

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Electronic control of H⁺ current in a bioprotonic device with Gramicidin A and Alamethicin

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